Biodegradation and toxicity removal of phthalate mixture by Gordonia sp. in a continuous stirred tank bioreactor system

Kanaujiya, Dipak Kumar; Sivashanmugam, Selvanayaki; Pakshirajan, Kannan

doi:10.1016/j.eti.2022.102324

Cited by 14 publications

(8 citation statements)

References 38 publications

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“…Compared to the high molecular weight (HMW) phthalate (DnOP), low molecular weight (LMW) phthalates (DMP and DBP) contain simple structures and are quickly degraded, even at high initial concentrations ( Figure 4 ). The literature reported values are consistent with the findings of this study [ 31 ]. It is known that the long ester side chains of high molecular weight phthalates pose a steric hindrance to the hydrolytic enzymes of the degrading bacteria, resulting in their low degradation rates [ 28 , 45 ].…”

Section: Resultssupporting

confidence: 93%

“…Gordonia sp. used in this biodegradation study was previously reported in our earlier study [ 31 ] and procured from Bose Institute (Kolkata, India). For biomass growth of the culture, Luria Bertani (LB) broth medium was used and the culture conditions were 30°C temperature & 150 rpm shaking in an orbital incubator shaker.…”

Section: Materials and Methodologymentioning

confidence: 99%

“…Biomass growth of Gordonia sp. was determined by UV-visible spectroscopy using Evolution 201 TM spectrophotometer (Thermo Scientific, USA) set at a wavelength of 660 nm [ 31 ]. The biomass concentration was calculated using a calibration curve that was previously generated between the culture’s dry biomass weight (DBW) and OD 660 .…”

Section: Methodsmentioning

confidence: 99%

“…The extracted samples were kept at room temperature to let the solvent evaporate. The details of the sample preparation were reported in our earlier work [ 31 ]. Afterward, methanol was added to the samples in equal amounts to dissolve phthalates.…”

Section: Methodsmentioning

confidence: 99%

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Biodegradation of low, medium and high molecular weight phthalate by Gordonia sp. in a batch system: Kinetics and phytotoxicity analyses

Kanaujiya

Chhantyal

et al. 2023

Bioengineered

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Phthalic acid esters (PAEs) are highly toxic compounds and can disrupt the hormonal balance of human, animal, and aquatic organisms. Due to the hazardous nature of such compounds, their removal from constituent wastewater before discharging into the environment is mandatory. This study focused on the biodegradation of dimethyl phthalates (DMP), di-n-butyl phthalates (DBP), and di-n-octyl phthalates (DnOP) by Gordonia sp. in a batch system. Initially, five different concentrations of DBP, DMP, and DnOP (200–1000 mg/L) were chosen individually as the sole carbon source to examine their effect on the biodegradation and biomass growth of Gordonia sp. Complete degradation of DBP and DMP was achieved up to 1000 mg/L initial concentration within 96 h, whereas in case of DnOP, the degradation value was only 83.5% at 120 h for the same initial concentration. The experimental data were fitted into various substrate inhibition kinetic models, and accurate predicted values of degradation of all the three PAEs were obtained using the Tiesser model in comparison with other models, which yielded the highest and lowest R 2 and SSE values of 0.99 and 0.02 × 10 −4 , respectively. In addition, the phytotoxicity of PAEs degraded samples was assessed and more than 50% germination index value was observed for DMP and DBP degraded sample which established the treatment efficiency of Gordonia sp. in degrading DMP and DBP. Hence, high DMP and DEP degradation and phytotoxicity removal efficiency of Gordonia sp. demonstrate its potential for the treatment of PAEs contaminated wastewater.

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Section: Resultssupporting

confidence: 93%

Section: Materials and Methodologymentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Biodegradation of low, medium and high molecular weight phthalate by Gordonia sp. in a batch system: Kinetics and phytotoxicity analyses

Kanaujiya

Chhantyal

et al. 2023

Bioengineered

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“…The mixtures were cultivated at 180 rpm, pH 8.0 and 30 °C for 5 days. The concentration of residual PAEs was determined using HPLC, and the degradation metabolites were detected by HPLC-MS [ 63 ].…”

Section: Methodsmentioning

confidence: 99%

A Novel and Efficient Phthalate Hydrolase from Acinetobacter sp. LUNF3: Molecular Cloning, Characterization and Catalytic Mechanism

Fan,

Guo,

Han

et al. 2023

Molecules

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Phthalic acid esters (PAEs), which are widespread environmental contaminants, can be efficiently biodegraded, mediated by enzymes such as hydrolases. Despite great advances in the characterization of PAE hydrolases, which are the most important enzymes in the process of PAE degradation, their molecular catalytic mechanism has rarely been systematically investigated. Acinetobacter sp. LUNF3, which was isolated from contaminated soil in this study, demonstrated excellent PAE degradation at 30 °C and pH 5.0–11.0. After sequencing and annotating the complete genome, the gene dphAN1, encoding a novel putative PAE hydrolase, was identified with the conserved motifs catalytic triad (Ser201-Asp295-His325) and oxyanion hole (H127GGG130). DphAN1 can hydrolyze DEP (diethyl phthalate), DBP (dibutyl phthalate) and BBP (benzyl butyl phthalate). The high activity of DphAN1 was observed under a wide range of temperature (10–40 °C) and pH (6.0–9.0). Moreover, the metal ions (Fe2+, Mn2+, Cr2+ and Fe3+) and surfactant TritonX-100 significantly activated DphAN1, indicating a high adaptability and tolerance of DphAN1 to these chemicals. Molecular docking revealed the catalytic triad, oxyanion hole and other residues involved in binding DBP. The mutation of these residues reduced the activity of DphAN1, confirming their interaction with DBP. These results shed light on the catalytic mechanism of DphAN1 and may contribute to protein structural modification to improve catalytic efficiency in environment remediation.

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Endocrine Disruptors

Luján‐Facundo,

Ferrer‐Polonio,

Piá

et al. 2024

Handbook of Water Pollution

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Biodegradation and toxicity removal of phthalate mixture by Gordonia sp. in a continuous stirred tank bioreactor system

Cited by 14 publications

References 38 publications

Biodegradation of low, medium and high molecular weight phthalate by Gordonia sp. in a batch system: Kinetics and phytotoxicity analyses

Biodegradation of low, medium and high molecular weight phthalate by Gordonia sp. in a batch system: Kinetics and phytotoxicity analyses

A Novel and Efficient Phthalate Hydrolase from Acinetobacter sp. LUNF3: Molecular Cloning, Characterization and Catalytic Mechanism

Endocrine Disruptors

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