Biodegradation and Bioaccumulation of Decachlorobiphenyl (DCB) by Native Strain Pseudomonas extremaustralis ADA-5

López, Miguel Angel Gómez; Zenteno-Rojas, Adalberto; Martı́nez-Romero, Esperanza; Rincón-Molina, Clara Ivette; Vences‐Guzmán, Miguel Ángel; Rincón-Molina, Francisco Alexander; Manzano-Gómez, Luis Alberto; Rincón-Rosales, Reiner

doi:10.1007/s11270-021-05122-2

Cited by 5 publications

(8 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There were also 14 bacteria strains co-metabolizing biphenyl and PCBs isolated from environmental samples, belonging to the genera Pseudomonas and Rhodococcus (Takahito et al, 2016). And Pseudomonas extremaustralis ADA-5 can utilize decachlorobiphenyl (DCB) as the unique carbon source and degrade 9.75% DCB within 336 h (López et al, 2021). In previous studies, the white-rot fungi Pleurotus ostreatus was the most efficient known organism in PCBs degradation, decomposing 99.6% of low-chlorinated biphenyls (commercial mixture Delor 103 with 2.6% PentaCB) after 42 days and having the capability of breaking down penta– and hexachlorinated biphenyls (Čvančarová et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

The Mechanism Insight into Bacterial Degradation of Pentachlorobiphenyl

Ji,

Chang,

Wang

et al. 2024

Preprint

View full text Add to dashboard Cite

Bacterial degradation mechanism for high chlorinated pentachlorobiphenyl (PentaCB) with worse biodegradability has not been fully elucidated, which could limit the full remediation of PCBs-combined pollution. In this research, using enzymatic screening method, a new PentaCB-degrading bacterium M. paraoxydans that has not been reported was obtained. The characteristic of its intracellular enzymes, proteome and metabolome variation during PentaCB degradation were investigated systematically. The results showed that PentaCB (PCB101, 1 mg/L) degradation rate could arrive 23.9% within 4 h till complete degradation within 12 h. The intracellular enzyme compound was optimally active at pH 6.0. The 12 up-regulated characterized proteins involved ABC transporter substrate-binding protein, translocase protein TatA and signal peptidase I (SPase I) indicated that functional proteins for PentaCB degradation were present both in the cytoplasm and outer surface of cytoplasmic membrane. There were also 5 differential metabolites strongly associated with above proteins in which the up-regulated 1, 2, 4-benzenetriol was enriched into the degradation pathways of benzoate, chlorocyclohexane, chlorobenzene and aminobenzoate. Bacterial degradation of PentaCB necessitates transmembrane transport, energy consumption, protein export, biofilm formation and quorum sensing. These findings hold significant theory and application value for PCBs biodegradation.

show abstract

Section: Discussionmentioning

confidence: 99%

The Mechanism Insight into Bacterial Degradation of Pentachlorobiphenyl

Ji,

Chang,

Wang

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…The presented results were analysed using the Mann-Whitney U test with *p < 0.05. www.nature.com/scientificreports/ 73 conducted computational analysis, which revealed that an increased quantity of PG, whose glycerol polar head is larger than that of ethanolamine, and a decrease in the PE content could lead to increased membrane stability and reduced fluidity. The modification of membrane composition as a process of adapting microorganisms to toxic contaminants is well documented [74][75][76] . Similar results were obtained by Bernat et al 77 , where the influence of iturin A lipopeptide on the phospholipid composition of B. subtilis was determined.…”

Section: The Influence Of Mit and Pcmx On Cellular Membrane Modificationmentioning

confidence: 99%

In vitro study of the ecotoxicological risk of methylisothiazolinone and chloroxylenol towards soil bacteria

Nowak-Lange

Niedziałkowska

Bernat

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Methylisothiazolinone (MIT) and chloroxylenol (PCMX) are popular disinfectants often used in personal care products (PCPs). The unregulated discharge of these micropollutants into the environment, as well as the use of sewage sludge as fertilizer and reclaimed water in agriculture, poses a serious threat to ecosystems. However, research into their ecotoxicity towards nontarget organisms is very limited. In the present study, for the first time, the ecotoxicity of biocides to Pseudomonas putida, Pseudomonas moorei, Sphingomonas mali, and Bacillus subtilis was examined. The toxicity of MIT and PCMX was evaluated using the microdilution method, and their influence on the viability of bacterial cells was investigated by the AlamarBlue® test. The ability of the tested bacteria to form biofilms was examined by a microtiter plate assay. Intracellular reactive oxygen species (ROS) production was measured with CM-H2DCFDA. The effect of MIT and PCMX on phytohormone indole-3-acetic acid (IAA) production was determined by spectrophotometry and LC‒MS/MS techniques. The permeability of bacterial cell membranes was studied using the SYTOX Green assay. Changes in the phospholipid profile were analysed using LC‒MS/MS. The minimal inhibitory concentrations (MICs) values ranged from 3.907 to 15.625 mg L−1 for MIT and 62.5 to 250 mg L−1 for PCMX, indicating that MIT was more toxic. With increasing concentrations of MIT and PCMX, the cell viability, biofilm formation ability and phytohormone synthesis were maximally inhibited. Moreover, the growth of bacterial cell membrane permeability and a significantly increased content of ROS were observed, indicating that the exposure caused serious oxidative stress and homeostasis disorders. Additionally, modifications in the phospholipid profile were observed in response to the presence of sublethal concentrations of the chemicals. These results prove that the environmental threat posed by MIT and PCMX must be carefully monitored, especially as their use in PCPs is still growing.

show abstract

“…Strains DCB13, DCB28, and DCB104 were biologically stimulated in a liquid medium for their cellular adaptation and to increase the PCB-209 assimilation efficiency. Strains were inoculated individually in a 250 mL Erlenmeyer flask with a cotton wool stopper with 100 mL of a minimal medium composed of 2.0 g/L (NH 4 ) 2 SO 4 , 1.0 g/L K 2 HPO 4 , 0.3 g/L NaCl, 0.3 g/L MgSO 4 7H 2 O, and 0.03 g/L FeSO 4 7H 2 O, to which 200 mg/L of biphenyl (as a sole carbon) was added in a proportion of 10% (v/v) in constant agitation at 120 rpm using an orbital shaker (Shaker ® , SD-300d (Suzhou, China) for 120 h [17]. The biomass produced was used as a preinoculum in flasks with a minimal medium to which 200 mg/L of PCB-209 was added in a proportion of 10% (v/v), keeping it under constant stirring at 120 rpm for 120 h at 30 • C. Bacterial growth was measured via serial dilutions (1 × 10 −1 to 1 × 10 −9 ) from 1.0 mL of the medium.…”

Section: Biostimulation Of Bacterial Strains In a Liquid Medium Conta...mentioning

confidence: 99%

“…Subsequently, 0.1 mL of each dilution was inoculated into a solid culture medium and incubated at 30 • C (50 L Luzeren ® digital incubator, Luzerne, PA, USA) for 36 h. This evaluation was performed on days 0, 3, 6, 9, 12, 15, 18, and 21 of the bioaugmentation process for all of the treatments. Bacterial growth was measured by plate count (CFU/g of soil) [17].…”

Section: Measurement Of Bacterial Growth In the Bioaugmentation Processmentioning

confidence: 99%

“…From another study, in agricultural and forest soils contaminated with PCBs, we isolated bacteria classified within the genera Burkholderia, Bacillus, Acinetobacter, Comamonas, and Cupriavidus, which showed high efficiency in removing and degrading PCB-209 as well as Aroclor 1260 (mixture of PCBs) [6]. We recently demonstrated that the native strain Pseudomonas extremaustralis ADA-5 has the biochemical ability to bioaccumulate and biodegrade PCB-209 at concentrations of 250 to 1000 mg/L [17]. We hypothesize that the native bacterial strains Bacillus sp.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Biostimulation and Bioaugmentation of Soils Contaminated with Decachlorobiphenyl (PCB-209) Using Native Bacterial Strains Individually and in Consortia

et al. 2022

Self Cite

View full text Add to dashboard Cite

Historically, microorganisms have proven to be efficient alternatives for the removal of PCBs, since these contaminants continue to be a major problem for human health and the environment. In this work, the removal of decachlorobiphenyl (PCB-209) was evaluated using native bacterial strains individually and in consortia through biostimulation and bioaugmentation processes. Bacillus sp. DCB13, Staphylococcus sp. DCB28, and Acinetobacter sp. DCB104 were biostimulated in a minimal medium that initially contained biphenyl and later PCB-209 for adaptation as a carbon source. The removal potential of PCB-209 by bacterial strains was evaluated in a bioaugmentation process under aerobic conditions. Using a completely randomized design, ten different treatments were evaluated. Finally, the bacterial growth (CFU/g of soil) and the chemical characteristics of the bioaugmented soil were determined, as was the content of PCB-209 removed by gas chromatography–mass spectrometry. Strains DCB13, DCB28, and DCB104 showed cell growth (>3.4 × 105 CFU/mL) during 120 h of biostimulation, with a marked difference between treatments with biphenyl compared with those where PCB-209 was added. Strains DCB13 and DCB104 (3.4 × 105 CFU/mL and 2.0 × 106 CFU/mL, respectively) grew better with PCB-209, while DCB28 grew better with biphenyl (4.5 × 106 CFU/mL). In bioaugmented soils contaminated with PCB-209, the strains showed maximum growth when inoculated in a consortium (>2.0 × 104 CFU/g). The results showe that the range of the bacterial elimination of PCB-209 in the treatments was from 9.58 to 17.33 mg/kg. The highest elimination potential of PCB-209 was obtained when the bacterial strains were inoculated in a consortium. These findings open a wide perspective for the use of native bacteria for the cleaning and restoration of soils contaminated by toxic chemicals.

show abstract

Biodegradation and Bioaccumulation of Decachlorobiphenyl (DCB) by Native Strain Pseudomonas extremaustralis ADA-5

Cited by 5 publications

References 42 publications

The Mechanism Insight into Bacterial Degradation of Pentachlorobiphenyl

The Mechanism Insight into Bacterial Degradation of Pentachlorobiphenyl

In vitro study of the ecotoxicological risk of methylisothiazolinone and chloroxylenol towards soil bacteria

Biostimulation and Bioaugmentation of Soils Contaminated with Decachlorobiphenyl (PCB-209) Using Native Bacterial Strains Individually and in Consortia

Contact Info

Product

Resources

About