Biodegradation ability and dioxgenase genes of PAH-degrading Sphingomonas and Mycobacterium strains isolated from mangrove sediments

Guo, Chuling; Dang, Zhi; Wong, Yuk Shan; Tam, N.F.Y.

doi:10.1016/j.ibiod.2010.04.008

Cited by 105 publications

(43 citation statements)

References 53 publications

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“…In petroleum-contaminated sediment, they also found that bacteria belonging to genus Paenibacillus can use PHE and naphthalene as sole carbon source (Daane et al 2002). On the other hand, Sphingomonas and Mycobacterium isolated from mangrove sediments are known to degrade phenanthrene (PHE), fluoranthene (Fla), and pyrene (Pyr) (Guo et al 2010). However, mangrove sediment itself had sufficient indigenous microorganisms which are capable of naturally remediating the PAH-contaminated environment, while bioaugmentation with the inoculation of PAH-degrading bacterial strains had little enhancement effect (Tam and Wong 2008).…”

Section: Diversity Of Pah-degrading Bacteria and Implication In Phytomentioning

confidence: 99%

Responses of endophytic and rhizospheric bacterial communities of salt marsh plant (Spartina alterniflora) to polycyclic aromatic hydrocarbons contamination

Ouyang

Hong

et al. 2015

J Soils Sediments

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Purpose Plants and their root-associated microbes play important roles in the remediation of polycyclic aromatic hydrocarbons (PAHs) present in the sediments of contaminated coastal wetlands. The detailed information about the effects of PAHs on root-associated bacterial communities could help to isolate PAH-degrading bacteria and optimize the process of phytoremediation. Materials and methods The community structures of rhizospheric (RB) and endophytic bacteria (EB) of salt marsh plant (Spartina alterniflora) grown in phenanthrene (PHE)-and pyrene (PYR)-contaminated sediments (for 70 days) were investigated using the barcoded Illumina paired-end sequencing technique. Results and discussion The diversity and community structure of EB and RB were more sensitive to PHE and PYR contamination as compared to those in the sediments. All samples were strongly clustered according to EB, RB, and sediments, suggesting the niche-specific influence of PAHs on bacterial communities. Potential PAH-degrading bacteria (Pseudomonas sp., Paenibacillus sp., and Flavobacterium sp.) in EB and RB were stimulated by PAH contamination. The results revealed an increase prevalence of specific bacteria which may be responsible for PAH degradation. PHE contamination increased the abundance of Proteobacteria in EB but decreased the number of Firmicutes, Bacteroidetes, and Chloroflexi. The numbers of Proteobacteria and Firmicutes in EB were enhanced by PYR treatment. However, both PHE and PYR treatment showed similar effects on the bacterial communities in RB.Conclusions The results suggested that PAH pollution could alter root-associated bacterial communities of S. alterniflora, whose EB might play an important role in the phytoremediation of PAH-contaminated sediments.

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Section: Diversity Of Pah-degrading Bacteria and Implication In Phytomentioning

confidence: 99%

Responses of endophytic and rhizospheric bacterial communities of salt marsh plant (Spartina alterniflora) to polycyclic aromatic hydrocarbons contamination

Ouyang

Hong

et al. 2015

J Soils Sediments

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“…Several species of bacteria have been confirmed that can utilize or degrade hydrocarbons. Mycobacterium strains have been shown to degrade 100% of a PAH mix, which included phenanthrene (Phe), fluoranthene (Fla) and pyrene (Pyr), in 14 days (Guo et al, 2010). Acinetobacter sp.…”

Section: Introductionmentioning

confidence: 99%

Crude oil depletion by bacterial strains isolated from a petroleum hydrocarbon impacted solid waste management site in California

Xia

Liu

Taylor

et al. 2017

International Biodeterioration & Biodegradation

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a b s t r a c tThis research is part of a multidisciplinary research program to develop a bioremediation protocol for a solid waste management (SWM) site in Northern California -a site which is heavily contaminated with petroleum hydrocarbons. In this initial study, 30 bacterial strains were isolated and evaluated for their efficiencies to deplete crude oil. The 3 most efficient bacterial isolates for crude oil depletion were designated as S1BD1, OPKDS2, and OSDS1; they were identified as Serratia proteamaculans, Alcaligenes sp. and Rhodococcus erythropolis, respectively, based on partial 16S rRNA gene sequences. Determination of crude oil depletion efficiency by gas chromatographyemass spectrometry (GC-MS) revealed that Serratia proteamaculans S1BD1 was the most efficient (68.0 ± 1.78%), followed by Alcaligenes sp. OPKDS2 (63.7 ± 3.28%), and Rhodococcus erythropolis OSDS1 (54.9 ± 5.07%). S. proteamaculans S1BD1 was able to deplete a wide spectrum of carbon compounds within the individual components of crude oil. Alcaligenes sp. OPKDS2 was the most efficient at depleting BTEX (91.2 ± 1.90%), and R. erythropolis OSDS1 exhibited a substrate preference of n-alkanes. All three strains exhibited unusually high crude oil depletion efficiencies and tolerated a wide range of salinity and pH levels, which makes them excellent candidates for bioaugmentation of the SWM site.

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“…NahAc gene is highly conserved among different Gram-negative bacteria (Park & Crowley, 2006). It was obtained from Gram negative bacteria such as Pseudomonas and Burkholderia species (Guo et al, 2010). This study gives a different result that this gene could obtain from G. cholesterolivorans which is Gram-positive bacteria.…”

Section: Resultsmentioning

confidence: 92%

“…A biodegradation study of PAH by Guo et al (2010) revealed that more than 80% of each of the three PAHs (Pyrene, Phenanthtene and Fluoranthene) was degraded by Mycobacterium after 7 days and pyrene was completely degraded by three Mycobacterium strains (SBSW, YOWG and SKEY) after 14 days. Mycobacterium showed higher ability in degrading the three PAHs than genera Sphingomonas, Rhodococcus, Paracoccus and Pseudomonas.…”

Section: Resultsmentioning

confidence: 99%

Degradation of Polycyclic Aromatic Hydrocarbon Pyrene by Biosurfactant-Producing Bacteria Gordonia cholesterolivorans AMP 10

et al. 2016

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Pyrene degradation and biosurfactant activity by a new strain identified as Gordonia cholesterolivorans AMP 10 were studied. The strain grew well and produced effective biosurfactants in the presence of glucose, sucrose, and crude oil. The biosurfactants production was detected by the decreased surface tension of the medium and emulsification activity. Analysis of microbial growth parameters showed that AMP10 grew best at 50 µg mL -1 pyrene concentration, leading to 96 % degradation of pyrene within 7 days. The result of nested PCR analysis revealed that this isolate possessed the nahAc gene which encodes dioxygenase enzyme for initial degradation of Polycyclic Aromatic Hydrocarbon (PAH). Observation of both tensio-active and emulsifying activities indicated that biosurfactants which produced by AMP 10 when grown on glucose could lower the surface tension of medium from 71.3 mN/m to 24.7 mN/m and formed a stable emulsion in used lubricant oil with an emulsification index (E24) of 74%. According to the results, it is suggested that the bacterial isolates G. cholesterolivorans AMP10 are suitable candidates for bioremediation of PAH-contaminated environments.

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Biodegradation ability and dioxgenase genes of PAH-degrading Sphingomonas and Mycobacterium strains isolated from mangrove sediments

Cited by 105 publications

References 53 publications

Responses of endophytic and rhizospheric bacterial communities of salt marsh plant (Spartina alterniflora) to polycyclic aromatic hydrocarbons contamination

Responses of endophytic and rhizospheric bacterial communities of salt marsh plant (Spartina alterniflora) to polycyclic aromatic hydrocarbons contamination

Crude oil depletion by bacterial strains isolated from a petroleum hydrocarbon impacted solid waste management site in California

Degradation of Polycyclic Aromatic Hydrocarbon Pyrene by Biosurfactant-Producing Bacteria Gordonia cholesterolivorans AMP 10

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