Biodegradable zwitterionic nanoparticles with tunable UCST-type phase separation under physiological conditions

Sponchioni, Mattia; Bassam, Paola Rodrigues; Moscatelli, Davide; Arosio, Paolo; Palmiero, Umberto Capasso

doi:10.1039/c9nr04311j

Cited by 40 publications

(37 citation statements)

References 50 publications

(32 reference statements)

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“…In order to verify whether these nanogel systems are able to encapsulate and tune the delivery of drugs with different properties, we loaded within them three drug mimetics commonly used in literature [ 41 , 42 , 43 ]. We used sodium fluorescein (SF) and rhodamine B (RhB) as hydrophilic drug mimetic and pyrene as hydrophobic drug mimetic.…”

Section: Results and Debatementioning

confidence: 99%

“…Three different drug mimetics were employed in drug loading and delivery tests. Sodium fluorescein (SF) and rhodamine B (RhB) were used as hydrophilic drug mimetics, while pyrene was used as hydrophobic drug mimetic [ 41 , 42 , 43 ].…”

Section: Methodsmentioning

confidence: 99%

“…Percentages of released hydrophilic drug molecules (rhodamine B, sodium fluorescein) and a hydrophobic one (pyrene) were then measured by UV spectroscopy at a specific wavelength. In the cases of SF ( λ = 485 nm) and RhB ( λ = 570 nm), aliquots were directly analyzed; for the test with pyrene ( λ = 334 nm), the aliquots were lyophilized and then solved in acetonitrile before being analyzed [ 42 ]. Loading efficiency (% loading) was calculated referring to Equation (1):

…”

Section: Methodsmentioning

confidence: 99%

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Influence of the Core Formulation on Features and Drug Delivery Ability of Carbamate-Based Nanogels

Pinelli

Pizzetti

Ortolà

et al. 2020

IJMS

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In the last years, nanogels have emerged as one of the most promising classes of novel drug delivery vehicles since they can be employed in multiple fields, such as various therapeutics or diagnostics, and with different classes of compounds and active molecules. Their features, such as a high volume to surface ratio, excellent drug loading and release ability, as well as biocompatibility and tunable behavior, are unique, and, nowadays, great efforts are made to develop new formulations that can be employed in a wider range of applications. Polyethylene glycol (PEG)-polyethylenimine (PEI) nanogels probably represent the baseline of this class of biomaterials and they are still largely employed and studied. In any way, the possibility to exploit new core formulations for nanogels is certainly very interesting in order to understand the influence of different polymer chains on the final properties of the system. In this research, we explore and make a comparison between PEG-PEI nanogels and two other different formulations: pluronic F127-PEI nanogels and PEG-Jeffamine nanogels. We propose nanogels synthesis methods, their chemical and physical characterization, as well as their stability analysis, and we focus on the different drug delivery ability that these structures exhibit working with different typologies of drug mimetics.

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Section: Results and Debatementioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

…”

Section: Methodsmentioning

confidence: 99%

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Influence of the Core Formulation on Features and Drug Delivery Ability of Carbamate-Based Nanogels

Pinelli

Pizzetti

Ortolà

et al. 2020

IJMS

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“…© 2020 Wiley-VCH GmbH to control their main characteristics, such as the micelle size and block-copolymer molecular weight, just by playing with the stoichiometry. [25] Therefore, in this work, the recently discovered possibility of functionalizing graphene with pyrrole rings was exploited to encapsulate few layers of this sp 2 carbon allotrope with unaltered bulk structure in micelles obtained from the selfassembly of pyrrole-based amphiphilic block copolymers produced via RAFT polymerization. This enabled the generation of nanosized reactors suitable for acid-catalyzed organic synthesis.…”

Section: (2 Of 6)mentioning

confidence: 99%

“…[ 23,24 ] This polymerization enables the synthesis of highly controllable and well‐defined amphiphilic block copolymers able to self‐assemble into micelles once dissolved in aqueous media and, in turn, to control their main characteristics, such as the micelle size and block‐copolymer molecular weight, just by playing with the stoichiometry. [ 25 ]…”

Section: Figurementioning

confidence: 99%

A Graphene‐Based Supramolecular Nanoreactor for the Fast Synthesis of Imines in Water

Palmiero

Sponchioni

Margani

et al. 2020

Small

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The confinement of organic synthesis within waterborne nanoreactors is regarded with increasing attention to improve its yield and reduce the environmental impact. However, many catalysts, such as graphene, are barely dispersible in aqueous media and many chemical reactions cannot be performed in the presence of water due to thermodynamic limitations. Therefore, there is an urgent need to develop novel strategies to carry out these processes in more sustainable conditions. To pursue this goal, in this work, a waterborne supramolecular nanoreactor is developed. The system comprises a polymeric micelle obtained from the self‐assembly of pyrrole‐based amphiphilic block copolymers. The active catalytic component is represented by few graphene layers, functionalized with pyrrole to enhance their interaction with the micelle core and hence their nanoencapsulation. Using this nanoreactor, it is possible to synthesize imines starting from primary amines and aldehydes or ketones with high yield and in short time (Y = 90% after 5 min) at room temperature. Moreover, an efficient strategy to recycle the reactor is proposed, thus increasing the potential of this technology.

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Programmable Zwitterionic Droplets as Biomolecular Sorters and Model of Membraneless Organelles

et al. 2021

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Increasing evidence indicates that cells can regulate biochemical functions in time and space by generating membraneless compartments with well‐defined mesoscopic properties. One important mechanism underlying this control is simple coacervation driven by associative disordered proteins that encode multivalent interactions. Inspired by these observations, programmable droplets based on simple coacervation of responsive synthetic polymers that mimic the “stickers‐and‐spacers” architecture of biological disordered proteins are developed. Zwitterionic polymers that undergo an enthalpy‐driven liquid–liquid phase separation process and form liquid droplets that remarkably exclude most molecules are developed. Starting from this reference material, different functional groups in the zwitterionic polymer are progressively added to encode an increasing number of different intermolecular interactions. This strategy allowed the multiple emerging properties of the droplets to be controlled independently, such as stimulus‐responsiveness, polarity, selective uptake of client molecules, fusion times, and miscibility. By exploiting this high programmability, a model of cellular compartmentalization is reproduced and droplets capable of confining different molecules in space without physical barriers are generated. Moreover, these biomolecular sorters are demonstrated to be able to localize, separate, and enable the detection of target molecules even within complex mixtures, opening attractive applications in bioseparation, and diagnostics.

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Biodegradable zwitterionic nanoparticles with tunable UCST-type phase separation under physiological conditions

Abstract: We report tunable biodegradable zwitterionic nanoparticles with UCST behavior under physiological conditions that can be used in controlled delivery strategies.

Cited by 40 publications

References 50 publications

Influence of the Core Formulation on Features and Drug Delivery Ability of Carbamate-Based Nanogels

Influence of the Core Formulation on Features and Drug Delivery Ability of Carbamate-Based Nanogels

A Graphene‐Based Supramolecular Nanoreactor for the Fast Synthesis of Imines in Water

Programmable Zwitterionic Droplets as Biomolecular Sorters and Model of Membraneless Organelles

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