Biodegradable Stearylated Peptide with Internal Disulfide Bonds for Efficient Delivery of siRNA In Vitro and In Vivo

Tai, Zongguang; Wang, Xiaoyu; Tian, Jing; Gao, Yuan; Zhang, Lijuan; Yan, Chong; Wu, Xin; Zhang, Wei; Zhu, Quangang; Gao, Shen

doi:10.1021/bm501777a

Cited by 58 publications

(52 citation statements)

References 54 publications

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“…Such alternations are generally termed as carrier mediated “off-target effects”. Reports also suggest that even non-ionic polymers such as PEG and PEG-poly-glutamic acid block copolymers can also cause non-specific gene alterations [146]. Therefore, it is very crucial to study the effect of polymer materials to gene signatures prior to its in vivo evaluation.…”

Section: Tumor Microenvironment In Lung Cancer Therapymentioning

confidence: 99%

Polymeric Nanoparticle-Mediated Gene Delivery for Lung Cancer Treatment

et al. 2017

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In recent years, researchers have focused on targeted gene therapy for lung cancer, using nanoparticle carriers to overcome the limitations of conventional treatment methods. The main goal of targeted gene therapy is to develop more efficient therapeutic strategies by improving the bioavailability, stability, and target specificity of gene therapeutics and to reduce off-target effects. Polymer-based nanoparticles, an alternative to lipid and inorganic nanoparticles, efficiently carry nucleic acid therapeutics and are stable in vivo. Receptor-targeted delivery is a promising approach that can limit non-specific gene delivery and can be achieved by modifying the polymer nanoparticle surface with specific receptor ligands or antibodies. This review highlights the recent developments in gene delivery using synthetic and natural polymer-based nucleic acid carriers for lung cancer treatment. Various nanoparticle systems based on polymers and polymer combinations are discussed. Further, examples of targeting ligands or moieties used in targeted, polymer-based gene delivery to lung cancer are reviewed.

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Section: Tumor Microenvironment In Lung Cancer Therapymentioning

confidence: 99%

Polymeric Nanoparticle-Mediated Gene Delivery for Lung Cancer Treatment

et al. 2017

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“…Cationic lipids, polymers, dendrimers, dendrimer‐like structures, peptides, nanocarriers, nanoparticles, nanocapsules, and supramolecular complexes are examples of carrier vehicles that transport siRNA into cells. However, efficiency, toxicity and stability in serum need to be further improved.…”

Section: Figurementioning

confidence: 99%

Efficient Transfection of siRNA by Peptide Dendrimer–Lipid Conjugates

et al. 2016

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Efficient delivery of small interfering RNA (siRNA) into cells is the basis of target-gene-specific silencing and, ultimately, gene therapy. However, current transfection reagents are relatively inefficient, and very few studies provide the sort of systematic understanding based on structure-activity relationships that would provide rationales for their improvement. This work established peptide dendrimers (administered with cationic lipids) as siRNA transfection reagents and recorded structure-activity relationships that highlighted the importance of positive charge distribution in the two outer layers and a hydrophobic core as key features for efficient performance. These dendrimer-based transfection reagents work as well as highly optimised commercial reagents, yet show less toxicity and fewer off-target effects. Additionally, the degrees of freedom in the synthetic procedure will allow the placing of decisive recognition features to enhance and fine-tune transfection and cell specificity in the future.

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“…Recent developments in reversibly cross-linked disulphide micelles have shown high stability in the circulation and effective drug release into tumour cells1819. Our previous studies202122 also demonstrated that disulphide-cross-linked micelles were capable of effectively carrying siRNA to target cells. In addition, other studies23242526 also reported that some disulphide micelles comprised lipoic acid conjugates could be used as vectors for drug delivery.…”

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confidence: 91%

Tumour microenvironment-responsive lipoic acid nanoparticles for targeted delivery of docetaxel to lung cancer

Tai

et al. 2016

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In the present study, we developed a novel type of reduction-sensitive nanoparticles (NPs) for docetaxel (DTX) delivery based on cross-linked lipoic acid NPs (LANPs). The physicochemical properties, cellular uptake and in vitro cytotoxicity of DTX loaded LANPs (DTX-LANPs) on A549 cells were investigated. Furthermore, the in vivo distribution and in vivo efficacy of DTX-LANPs was evaluated. The results showed that DTX-LANPs had a particle size of 110 nm and a negative zeta potential of −35 mv with excellent colloidal stability. LANPs efficiently encapsulated DTX with a high drug loading of 4.51% ± 0.49% and showed remarkable reduction-sensitive drug release in vitro. Cellular uptake experiments demonstrated that LANPs significantly increased intracellular DTX uptake by about 10 fold as compared with free DTX. The cytotoxicity of DTX-LANPs showed significantly higher potency in inhibiting A549 cell growth than free DTX, while blank LANPs had a good biocompatibility. In addition, in vivo experiments demonstrated that DTX-LANPs could enhance tumour targeting and anti-tumour efficacy with low systemic toxicity. In conclusion, LANPs may prove to be a potential tumour microenvironment-responsive delivery system for cancer treatment, with the potential for commercialization due to the simple component, controllable synthesis, stability and economy.

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Biodegradable Stearylated Peptide with Internal Disulfide Bonds for Efficient Delivery of siRNA In Vitro and In Vivo

Cited by 58 publications

References 54 publications

Polymeric Nanoparticle-Mediated Gene Delivery for Lung Cancer Treatment

Polymeric Nanoparticle-Mediated Gene Delivery for Lung Cancer Treatment

Efficient Transfection of siRNA by Peptide Dendrimer–Lipid Conjugates

Tumour microenvironment-responsive lipoic acid nanoparticles for targeted delivery of docetaxel to lung cancer

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