Biodegradable poly-l-lactide based microparticles as controlled release delivery system for filarial vaccine candidate antigens

Anugraha, Gandhirajan; Madhumathi, Jayaprakasam; Prita, Parasurama Jawaharlal Jeya; Kaliraj, Perumal

doi:10.1016/j.ejphar.2014.12.004

Cited by 15 publications

(9 citation statements)

References 38 publications

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“…The amount of polymer, nature of the solvent, preparation methods, and stirring rate influences the size of the particle. As reported by Howie et al and Anugraha et al, larger size particles have less effect and smaller size particles have chances of more positivity (Howie et al, 1993;Anugraha et al, 2015). Microspheres ranging from 10 to 200 µm diameters achieved optimal release as reported by Han et al (Han et al, 2016).…”

Section: Discussionsupporting

confidence: 52%

“…PLA encapsulation was carried out with the double emulsion solvent evaporation method (w/o/w) with the modifications from Rui at al. (Rui et al, 2012;Anugraha et al, 2015). The size of the PLA particles ranged from 11.2 to 23.9 µm.…”

Section: Discussionmentioning

confidence: 99%

“…Microparticles containing VEGF was prepared by double emulsion solvent evaporation method (w/o/w) as described by in the literature with slight modifications (Anugraha et al, 2015;Rui et al, 2012). Briefly, 5 µg of rVEGF 121 was dissolved separately in 0.25 mL of 10% polyvinyl alcohol (w/v) (PVA) (M.W.…”

Section: Preparation Of Vegf-containing Microparticlesmentioning

confidence: 99%

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Evaluation of recombinant human Vascular Endothelial Growth Factor VEGF121-loaded poly-l-lactide microparticles as a controlled release delivery system

Abraham

Rangaswamy²,

Amutha

2020

Turk J Biol

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Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 99%

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Evaluation of recombinant human Vascular Endothelial Growth Factor VEGF121-loaded poly-l-lactide microparticles as a controlled release delivery system

Abraham

Rangaswamy²,

Amutha

2020

Turk J Biol

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“…It has been reported that PLLA-based scaffolds with HA coated on the surface of nanofibers enhanced the osteogenic differentiation of unrestricted somatic stem cells. 20 Collagen (Coll), as another major component of bone ECM, can enhance cell adhesion, proliferation, and differentiation. The Coll/HA composites not only showed a favorable biocompatibility but also enhanced the bone marrow stromal cell adhesion and differentiation.…”

mentioning

confidence: 99%

Innovative biodegradable poly(L-lactide)/collagen/<br />hydroxyapatite composite fibrous scaffolds promote osteoblastic proliferation and differentiation

Zhou¹,

Liu²,

Ma³

et al. 2017

IJN

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The development of an artificial bone graft which can promote the regeneration of fractures or diseased bones is currently the most challenging aspect in bone tissue engineering. To achieve the purpose of promoting bone proliferation and differentiation, the artificial graft needs have a similar structure and composition of extracellular matrix. One-step electrospinning method of biocomposite nanofibers containing hydroxyapatite (HA) nanoparticles and collagen (Coll) were developed for potential application in bone tissue engineering. Nanocomposite scaffolds of poly(L-lactide) (PLLA), PLLA/HA, PLLA/Coll, and PLLA/Coll/HA were fabricated by electrospinning. The morphology, diameter, elements, hydrophilicity, and biodegradability of the composite scaffolds have been investigated. The biocompatibility of different nanocomposite scaffolds was assessed using mouse osteoblasts MC3T3-E1 in vitro, and the proliferation, differentiation, and mineralization of cells on different nanofibrous scaffolds were investigated. The results showed that PLLA/Coll/HA nanofiber scaffolds enhanced cell adhesion, spreading, proliferation, differentiation, mineralization, and gene expression of osteogenic markers compared to other scaffolds. In addition, the nanofibrous scaffolds maintained a stable composition at the beginning of the degradation period and morphology wastage and weight loss were observed when incubated for up to 80 days in physiological simulated conditions. The PLLA/Coll/HA composite nanofibrous scaffolds could be a potential material for guided bone regeneration.

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“…In comparison with earlier observations, our studies suggest the prophylactic efficiency of FEP against B. malayi with infective L3 larvae, containing only immunodominant epitopes which are non‐homologous to the host and non‐host immunosuppressive can be considered as a potential vaccine candidate over other individual parasitic proteins. In a parallel study, the immune responses of FEP in mice, encapsulated in a biodegradable microparticle poly‐l‐lactide (PLA)‐based delivery system, elicited strong and protective humoral as well as cellular response and immunogenic to generate long‐lasting immune response in PLA formulation . Unlike other vaccine candidates, FEP uses a multipronged approach to tackle the survival of parasite at different stages and also effectively activates both humoral and cell‐mediated aspects of the immune system to enhance the immune response.…”

Section: Resultsmentioning

confidence: 99%

Chimeric Epitope Vaccine from Multistage Antigens for Lymphatic Filariasis

et al. 2015

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Lymphatic filariasis, a mosquito-borne parasitic disease, affects more than 120 million people worldwide. Vaccination for filariasis by targeting different stages of the parasite will be a boon to the existing MDA efforts of WHO which required repeated administration of the drug to reduce the infection level and sustained transmission. Onset of a filaria-specific immune response achieved through antigen vaccines can act synergistically with these drugs to enhance the parasite killing. Multi-epitope vaccine approach has been proved to be successful against several parasitic diseases as it overcomes the limitations associated with the whole antigen vaccines. Earlier results from our group suggested the protective efficacy of multi-epitope vaccine comprising two immunodominant epitopes from Brugia malayi antioxidant thioredoxin (TRX), several epitopes from transglutaminase (TGA) and abundant larval transcript-2 (ALT-2). In this study, the prophylactic efficacy of the filarial epitope protein (FEP), a chimera of selective epitopes identified from our earlier study, was tested in a murine model (jird) of filariasis with L3 larvae. FEP conferred a significantly (P < 0.0001) high protection (69.5%) over the control in jirds. We also observed that the multiepitope recombinant construct (FEP) induces multiple types of protective immune responses, thus ensuring the successful elimination of the parasite; this poses FEP as a potential vaccine candidate.

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Biodegradable poly-l-lactide based microparticles as controlled release delivery system for filarial vaccine candidate antigens

Cited by 15 publications

References 38 publications

Evaluation of recombinant human Vascular Endothelial Growth Factor VEGF121-loaded poly-l-lactide microparticles as a controlled release delivery system

Evaluation of recombinant human Vascular Endothelial Growth Factor VEGF121-loaded poly-l-lactide microparticles as a controlled release delivery system

Innovative biodegradable poly(L-lactide)/collagen/<br />hydroxyapatite composite fibrous scaffolds promote osteoblastic proliferation and differentiation

Chimeric Epitope Vaccine from Multistage Antigens for Lymphatic Filariasis

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