Biodegradable Nanocomposite Antimicrobials for the Eradication of Multidrug-Resistant Bacterial Biofilms without Accumulated Resistance

Landis, Ryan F.; Li, Cheng‐Hsuan; Gupta, Akash; Lee, Yi-Wei; Yazdani, Mahdieh; Ngernyuang, Nipaporn; Altinbasak, Ismail; Mansoor, Sanaa; Khichi, Muhammadaha A. S.; Sanyal, Amitav; Rotello, Vincent M.

doi:10.1021/jacs.8b03575

Cited by 95 publications

(84 citation statements)

References 46 publications

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“…Finally, the selective bacterial eradication and treatment efficacy of the Ab@EO NCs was evaluated in an in vitro coculture model composed of the target S. aureus bacterium and human skin fibroblasts, which are critical for skin healing and recovery. 43 Human cells were infected with S. aureus and then exposed to the Ab@EO NCs at their defined antibacterial dosage. Afterward, S. aureus bacterial growth was quantified via a plate count method, while the viability of the skin fibroblasts was determined by a live/dead viability/cytotoxicity assay kit.…”

Section: Results and Discussionmentioning

confidence: 99%

Antibody-Enabled Antimicrobial Nanocapsules for Selective Elimination of Staphylococcus aureus

Ivanova

Ramon

et al. 2020

ACS Appl. Mater. Interfaces

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Targeted bactericide nanosystems hold significant promise to improve the efficacy of existing antimicrobials for treatment of severe bacterial infections, minimizing the side effects and lowering the risk of the development of antibiotic resistance. In this work, we developed antibody-functionalized nanocapsules (NCs) containing antibacterial essential oil (EO) for selective and effective eradication of Staphylococcus aureus . Antibacterial EO NCs were produced via self-assembly nanoencapsulation in the plant-derived protein zein. The obtained EO NCs were decorated with aminocellulose to provide more reactive surface groups for carboxyl-to-amine immobilization of a antibody that is specific against S. aureus . The antibody-enabled EO NCs (Ab@EO NCs) demonstrated 2-fold higher bactericidal efficacy against the targeted bacterium compared to the pristine EO NCs at the same concentrations. The improved antibacterial effect of the Ab@EO NCs toward S. aureus was also confirmed in a real-time assay by monitoring bacterial cells elimination using a quartz crystal microbalance. Furthermore, the Ab@EO NCs selectively decreased the load and changed the cell morphology of the targeted S. aureus in a mixed inoculum with nontargeted Pseudomonas aeruginosa . Applying the nanoformulated antibacterial actives to an in vitro coculture model of the bacteria and skin fibroblasts resulted in suppression of S. aureus growth while preserving the human cells viability. The novel antibody-enabled antibacterial NCs showed potential for improving the treatment efficacy of staphylococcal infections, minimally affecting the beneficial microbial and human cells.

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Section: Results and Discussionmentioning

confidence: 99%

Antibody-Enabled Antimicrobial Nanocapsules for Selective Elimination of Staphylococcus aureus

Ivanova

Ramon

et al. 2020

ACS Appl. Mater. Interfaces

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“…However, up to now, the adaptive tendency of pathogens on drug resistance produces a great threat to current therapeutic systems in combating diverse bacterial infections . Hence, novel and efficient antibacterial materials and strategies are urgently needed to reduce the dependence on antibiotic treatments . In recent years, abundant kinds of antibacterial materials and strategies have been developed, which not only include chemotherapies by using diverse small molecules, quaternary ammonium polymers, and bactericidal metal ions (Ag 2+ , Zn 2+ , and Cu 2+ ions), but also consist of photothermal‐ or photodynamic‐based sterilization via production of massive heating or reactive oxygen species .…”

Section: Introductionmentioning

confidence: 99%

Size‐Transformable Metal–Organic Framework–Derived Nanocarbons for Localized Chemo‐Photothermal Bacterial Ablation and Wound Disinfection

Yang

Deng

Huang

et al. 2019

Adv Funct Materials

104

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Severe infectious diseases caused by pathogenic bacteria have become urgent threats to global public health. Antibacterial materials with combined chemophotothermal therapeutic capabilities possess distinct advantages when compared with many other antibacterial approaches. However, developing simplified and chemically tunable precursors to synthesize such antibacterial nanoagents for rapidly, safely, and synergistically combating pathogenic bacteria remains a huge challenge. Herein, metal-organic framework (MOF)-derived nanocarbons with near-infrared (NIR)-responsive and sizetransformable capabilities are designed to overcome this challenge. The MOF-derived nanocarbons with chemo-photothermal bactericidal capabilities are first synthesized, and then coated with a thermoresponsive gel layer to obtain ON-OFF switching capability for bacterial trapping. The fabricated nanocarbons exhibit high photo-to-thermal conversion efficiency and fast size transformation from nanodispersions to micrometer aggregations upon NIR irradiation, thus enabling nanocarbons to generate localized massive heat and abundant Zn 2+ ions for directly disrupting bacterial membrane and intracellular proteins. Furthermore, these nanocarbons not only exhibit a nearly 100% bactericidal ratio at very low dosage, but also possess highly efficient and safe wound disinfection activities, which are comparable to vancomycin. Overall, these proposed novel nanocarbons display robust and localized chemo-photothermal bactericidal capability and possess great potential to be used as alternative to antibiotics for broad-spectrum eradication of pathogenic bacteria.

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“…The eventual goal of these studies is to develop cost-effective, potent, safe, readily available, and easily synthesized alternatives to the conventional antibiotics that are increasingly becoming ineffective against MDR pathogens. 9−11…”

Section: Introductionmentioning

confidence: 99%

Cationic Silver Nanoclusters as Potent Antimicrobials against Multidrug-Resistant Bacteria

et al. 2018

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Bacterial multidrug resistance (MDR) is a serious healthcare issue caused by the long-term subtherapeutic clinical treatment of infectious diseases. Nanoscale engineering of metal nanoparticles has great potential to address this issue by tuning the nano–bio interface to target bacteria. Herein, we report the use of branched polyethylenimine-functionalized silver nanoclusters (bPEI–Ag NCs) to selectively kill MDR pathogenic bacteria by combining the antimicrobial activity of silver with the selective toxicity of bPEI toward bacteria. The minimum inhibitory concentration of bPEI–Ag NCs was determined against 12 uropathogenic MDR strains and found to be 10- to 15-fold lower than that of PEI and 2- to 3-fold lower than that of AgNO3 alone. Cell viability and hemolysis assays demonstrated the biocompatibility of bPEI–Ag NCs with human fibroblasts and red blood cells, with selective toxicity against MDR bacteria.

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Biodegradable Nanocomposite Antimicrobials for the Eradication of Multidrug-Resistant Bacterial Biofilms without Accumulated Resistance

Cited by 95 publications

References 46 publications

Antibody-Enabled Antimicrobial Nanocapsules for Selective Elimination of Staphylococcus aureus

Antibody-Enabled Antimicrobial Nanocapsules for Selective Elimination of Staphylococcus aureus

Size‐Transformable Metal–Organic Framework–Derived Nanocarbons for Localized Chemo‐Photothermal Bacterial Ablation and Wound Disinfection

Cationic Silver Nanoclusters as Potent Antimicrobials against Multidrug-Resistant Bacteria

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