Biodegradable Microcarriers of Poly(Lactide-co-Glycolide) and Nano-Hydroxyapatite Decorated with IGF-1 via Polydopamine Coating for Enhancing Cell Proliferation and Osteogenic Differentiation

Gao, Tianlin; Zhang, Ning; Wang, Zongliang; Wang, Yu; Ya, Liu; Ito, Yoshihiro; Zhang, Peibiao

doi:10.1002/mabi.201500069

Cited by 63 publications

(48 citation statements)

References 38 publications

(53 reference statements)

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“…Similarly, the specific outgrowth rate and mean cell diameter have been maintained post-harvest, demonstrating that the microcarrier process has not affected these BM-hMSC characteristics. In contrast, the osteogenic potential of the BM-hMSCs decreased for all conditions (P < 0.01), which should be further investigated if the BM-hMSCs are to be used for clinical indications relating to the production of collagen.There is the potential however, for this type of clinical indication, that biodegradable microcarriers could be directly implanted as a cellscaffold construct to support the regeneration of bone tissue [33], removing the need for cell harvest altogether. This comparison of performance demonstrates that HPL is a viable alternative to FBS for the microcarrier culture of BM-hMSCs and has the potential to be taken forward to support further process development and scale-up.The increased consistency in growth between donors in HPL will also benefit the development of both patient specific and off-the-shelf BM-hMSC therapies, allowing for increased production rates and shorter processing times.…”

Section: Microcarrier Expansion Of Bm-hmscsmentioning

confidence: 99%

Scalability and process transfer of mesenchymal stromal cell production from monolayer to microcarrier culture using human platelet lysate

et al. 2016

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Section: Microcarrier Expansion Of Bm-hmscsmentioning

confidence: 99%

Scalability and process transfer of mesenchymal stromal cell production from monolayer to microcarrier culture using human platelet lysate

et al. 2016

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“…The hMSCs seeded on nanocomposite PCL/HA scaffolds in 12-well plate and showed high proliferation in HA concentration-dependent manner as compared to Neat-PCL (Figure 4). Additionally, previous studies have demonstrated that PCL/HA-based scaffolds showed high proliferation and osteogenic differentiation in human dental pulp stem cells (hDPSCs) and mouse adipose-derived stem cells (ADSCs) (33, 34). Interestingly, magnetic bioinspired hybrid nanocomposite collagen-hydroxyapatite scaffolds enhanced cellular proliferation and restored bone generation (35, 36).…”

Section: Resultsmentioning

confidence: 99%

Biphasic organo-bioceramic fibrous composite as a biomimetic extracellular matrix for bone tissue regeneration

Mishra¹

2017

Front Biosci

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In bone tissue engineering, the organo-ceramic composite, electrospun polycaprolactone/hydroxyapatite (PCL/HA) scaffold has the potential to support cell proliferation, migration, differentiation, and homeostasis. Here, we report the effect of PCL/HA scaffold in tissue regeneration using human mesenchymal stem cells (hMSCs). We characterized the scaffold by fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), scanning electron microscope (SEM) and assessed its biocompatibility. PCL/HA composite is superior as a scaffold compared to PCL alone. Furthermore, increasing HA content (5–10%) was more efficacious in supporting cell-scaffold attachment, expression of ECM molecules and proliferation. These results suggest that PCL/HA is useful as a scaffold for tissue regeneration.

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“…Specific proteins and peptides that promote bone regrowth and healing have also been attached to implant materials through polydopamine coatings, resulting in strong immobilization and improved bone regeneration . This includes the attachment of growth factors to promote the attachment and osteogenic differentiation of adipose‐derived stem cells, enhancing healing and inhibiting scarring in rabbits . Also, bone morphogenetic protein and derived peptides have been absorbed onto polydopamine surfaces, leading to increased expression of markers of osteogenesis in human stem cells, and promoting bone formation in mice …”

Section: Applications Of Catecholamine Polymersmentioning

confidence: 99%

Versatile Surface Modification Using Polydopamine and Related Polycatecholamines: Chemistry, Structure, and Applications

Barclay

Hegab

Clarke

et al. 2017

Adv Materials Inter

292

223

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Almost ten years ago, Lee et al. [13] formulated a universal adhesive coating based on observation of the strong attachment by mussels through their byssal threads to virtually all types of inorganic and organic surfaces. The amino acid compositions of the mussel foot proteins that generate the attachment are rich in catechol and amine groups. [13][14][15] These groups associate under marine conditions, forming strong covalent and noncovalent interactions with substrates. [13] This led to the idea that both catechol and amine groups were crucial in forming robust, wide spectrum adhesive nanolayers [16,17] and consequently dopamine was conceived as a powerful building block for spontaneous deposition of thin polymer films. The dopamine monomer is deposited onto unadulterated surfaces through self-assembly and oxidative cross-linking from mild pH aqueous solution in a rapid reaction. This results in a durable, nanoscale, conformal, and hydrophilic coating that can be readily modified to introduce specific surface chemistries for a particular application. [18][19][20][21][22] These bioinspired, polydopamine materials are chemically and structurally indistinguishable from eumelanins found in the human body, [23,24] providing excellent biocompatibility. Additionally, polydopamine has broad electromagnetic absorption and excellent photothermal energy conversion [25] as well as having ionic-electronic hybrid conductivity. [26] Along with the excellent coating performance, these properties provide a vast array of potential applications for polydopamine materials.In this article, we explain what is known about polydopamine and related catecholamine coatings; their formation, the adhesion mechanism, and their physicochemical properties and we also review the applications of these materials (Figure 1). Since 2011, there have been a number of reviews on this subject matter, including general reviews on the physicochemical properties of polydopamine coatings [11,20,[27][28][29] and their applications. [20,27,30] There are also a number of more specific reviews on the chemical synthesis and modulation of polycatecholamine coatings, [31] the biomedical applications of these coatings, [8,[32][33][34] their electronic properties, [26,35] the use of polydopamine to make films at fluid interfaces, [36] in hierarchically constructed particles, [33] and capsules, [30] exploitation of polycatecholamine coatings in membrane filtration, [37] polydopamine-derived carbon materials, [38] and the use of coordination chemistry in catechol-based materials. [39] Nonetheless, this area of research is growing so rapidly [25,27] that many recent Polydopamine and related polycatecholamines can be easily deposited onto almost any surface from mild, aqueous solution. This results in durable, nanoscale coatings that exhibit high biocompatibility and have useful chemical and electronic properties. Additionally, these materials can be readily chemically and physically modified, and consequently, they are used extensively for surface modification. This ...

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Biodegradable Microcarriers of Poly(Lactide-co-Glycolide) and Nano-Hydroxyapatite Decorated with IGF-1 via Polydopamine Coating for Enhancing Cell Proliferation and Osteogenic Differentiation

Cited by 63 publications

References 38 publications

Scalability and process transfer of mesenchymal stromal cell production from monolayer to microcarrier culture using human platelet lysate

Scalability and process transfer of mesenchymal stromal cell production from monolayer to microcarrier culture using human platelet lysate

Biphasic organo-bioceramic fibrous composite as a biomimetic extracellular matrix for bone tissue regeneration

Versatile Surface Modification Using Polydopamine and Related Polycatecholamines: Chemistry, Structure, and Applications

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