Biodegradable magnesium materials regulate ROS-RNS balance in pro-inflammatory macrophage environment

Kwesiga, Maria Paula; Gillette, Amani A.; Razaviamri, Fatemeh; Plank, Margaret E.; Canull, Alexia L.; Alesch, Zachary; He, Weilue; Lee, Bruce P.; Guillory, Roger J.

doi:10.1016/j.bioactmat.2022.10.017

Cited by 12 publications

(5 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Monocyte/macrophage is considered the key cell for the initiation, duration, and consequences of foreign body reactions to implanted biomaterials 49 . These cellular inflammatory signaling pathways, ROS up‐regulation, and macrophage polarization play a crucial role, 50,51 although their exact molecular mechanism is unknown. The particles larger than 10 μm exceed the phagocytic capacity of macrophages and will not cause inflammatory reactions in macrophages 52 .…”

Section: Discussionmentioning

confidence: 99%

Effect of unsoluble corrosion products of WE43 alloys in vitro on macrophages

Dong,

Shen,

Zhang

et al. 2023

J Biomedical Materials Res

View full text Add to dashboard Cite

Magnesium alloys have been used to manufacture biodegradable implants, bone graft substitutes, and cardiovascular stents. WE43 was the most widely used magnesium alloy. The degradation process begins when the magnesium alloy stent is implanted in the body and comes into contact with body fluid. The degradation products include hydrogen, Mg2+, local alkaline environment, and unsoluble products. A large number of studies focused on Mg2+ and pH in vitro, and in vivo of magnesium alloys, but few studies on unsoluble corrosion products (UCPs). In this study, UCPs of WE43 alloy were prepared by immersion in vitro, and their effects on macrophages were investigated. The results showed that the unsoluble corrosion products were Mg24Y5, Mg12YNd, and MgCO3·3H2O, which were dose‐dependent on the apoptosis and necrosis of macrophages. After phagocytosis of UCPs, macrophages mainly metabolize in lysosome, and autophagy also participates in the metabolism of UCPs. It also decreases mitochondrial membrane potential and increases lysosomes, endoplasmic reticulum stress, and P2X7 receptor activation. These will increase reactive oxygen species (ROS) in cells, activating NLRP3 inflammatory corpuscles, activating the downstream pro‐IL18 and pro‐IL1β, and converting it to IL‐18, and IL‐1β. However, its pro‐inflammatory effect is far lower than that of the classical Lipopolysaccharide (LPS) pro‐inflammatory pathway. This work has increased our understanding of magnesium alloy metabolism and provides new ideas for the clinical application of magnesium alloys.

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of unsoluble corrosion products of WE43 alloys in vitro on macrophages

Dong,

Shen,

Zhang

et al. 2023

J Biomedical Materials Res

View full text Add to dashboard Cite

show abstract

“…Studies have shown that Mg 2+ is a key cofactor for enzyme activation and has been implicated in the process of regulating cellular ROS and inflammation levels [ 41 , 42 ]. Furthermore, recent research work has shown that the anti-inflammatory and antioxidant properties of Mg 2+ help in the treatment of inflammatory diseases [ 43 – 45 ]. In according to these, we observed that the Mg 2+ -charged NPCs exhibited satisfactory anti-ROS, anti-inflammatory, and anti-senescence abilities both in vitro and in vivo.…”

Section: Discussionmentioning

confidence: 99%

Transplantation of active nucleus pulposus cells with a keep-charging hydrogel microsphere system to rescue intervertebral disc degeneration

Tang,

Zhang,

Zhou

et al. 2023

J Nanobiotechnol

View full text Add to dashboard Cite

Background Cell transplantation has been demonstrated as a promising approach in tissue regeneration. However, the reactive oxygen species (ROS) accumulation and inflammation condition establish a harsh microenvironment in degenerated tissue, which makes the transplanted cells difficult to survive. Methods In this study, we constructed a keep-charging hydrogel microsphere system to enable cells actively proliferate and function in the degenerated intervertebral disc. Specifically, we combined Mg2+ to histidine-functionalized hyaluronic acid (HA-His-Mg2+) through coordination reaction, which was further intercrossed with GelMA to construct a double-network hydrogel microsphere (GelMA/HA-His-Mg2+, GHHM) with microfluidic methods. In vitro, the GHHM loaded with nucleus pulposus cells (GHHM@NPCs) was further tested for its ability to promote NPCs proliferation and anti-inflammatory properties. In vivo, the ability of GHHM@NPCs to promote regeneration of NP tissue and rescue intervertebral disc degeneration (IVDD) was evaluated by the rat intervertebral disc acupuncture model. Results The GHHM significantly enhanced NPCs adhesion and proliferation, providing an ideal platform for the NPCs to grow on. The loaded NPCs were kept active in the degenerative intervertebral disc microenvironment as charged by the Mg2+ in GHHM microspheres to effectively support the loaded NPCs to reply against the ROS-induced inflammation and senescence. Moreover, we observed that GHHM@NPCs effectively alleviated nucleus pulposus degeneration and promoted its regeneration in the rat IVDD model. Conclusion In conclusion, we constructed a keep charging system with a double-network hydrogel microsphere as a framework and Mg2+ as a cell activity enhancer, which effectively maintains NPCs active to fight against the harsh microenvironment in the degenerative intervertebral disc. The GHHM@NPCs system provides a promising approach for IVDD management. Graphical Abstract

show abstract

“…From another perspective, corrosion in the physiological environment is also controlled by the cells, inflammatory reactions and other biological responses [ [29] , [30] , [31] , [32] ]. On the retrieved metallic prostheses, inflammatory cell-like residues and corrosion damages were extensively detected [ [33] , [34] , [35] ].…”

Section: Introductionmentioning

confidence: 99%

Interaction pathways of implant metal localized corrosion and macrophage inflammatory reactions

Li,

Wu,

Geng

et al. 2024

Bioactive Materials

View full text Add to dashboard Cite

Biodegradable magnesium materials regulate ROS-RNS balance in pro-inflammatory macrophage environment

Cited by 12 publications

References 57 publications

Effect of unsoluble corrosion products of WE43 alloys in vitro on macrophages

Effect of unsoluble corrosion products of WE43 alloys in vitro on macrophages

Transplantation of active nucleus pulposus cells with a keep-charging hydrogel microsphere system to rescue intervertebral disc degeneration

Interaction pathways of implant metal localized corrosion and macrophage inflammatory reactions

Contact Info

Product

Resources

About