Biodegradable fibrous scaffolds composed of gelatin coated poly(ϵ‐caprolactone) prepared by coaxial electrospinning

Zhao, Pengcheng; Jiang, Hongliang; Pan, Hui; Zhu, Kun; Chen, Weiliam

doi:10.1002/jbm.a.31242

Cited by 113 publications

(79 citation statements)

References 48 publications

(51 reference statements)

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“…Coatings of collagen on PCL were shown to favor proliferation of human dermal fibroblasts and also encouraged cell migration inside the scaffolds. Using a similar approach, biodegradable fibrous scaffolds composed of gelatin coated PCL were prepared by Zhao et al by coaxial electrospinning [22]. More recently, Sun et al developed core-shell PAN-PMMA nanofibers by coaxial electrospinning [23].…”

Section: Coaxial Electrospinningmentioning

confidence: 99%

Biological Events Occuring on the Biosis–Abiosis Interface: Cellular Responses Induced by Implantable Electrospun Nanofibrous Scaffolds

Deng

Wei

Zhang

et al. 2015

Interface Oral Health Science 2014

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Electrospun nanofibers have tremendous potential as novel scaffolds for tissue engineering of various soft and hard tissues because of thier high surface area, surface functional groups, interconnected pores, and nano-scaled size. In this chapter, we reviewed the types of the nanofibrous scaffolds that have been used as implantable biomedical devices and used electrospun nanofibrous guided tissue regeneration membrane as an example to illustrate how the physiochemical properties of nanofibrous scaffolds influenced the biological events occuring on the scaffolds-host interface. It could be concluded that physical and chemical stimuli caused by nanofibrous scaffold would support in vivo-like three-dimensional cell adhesion and activate cell-signaling pathways. In terms of physical stimulus, the process of mechanotransduction may play an important role in influencing cellular behaviors. A result, biological events such as cell-interface recognition, cell proliferation, and cell differentiation are altered. Nevertheless, the cellular and molecular mechanisms by which cells sense and respond to nanofiberous scaffolds remain poorly understood. More evidences are needed to reveal the underlying mechanisms whereby environmental cues alternated the cellular responses to the

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Section: Coaxial Electrospinningmentioning

confidence: 99%

Biological Events Occuring on the Biosis–Abiosis Interface: Cellular Responses Induced by Implantable Electrospun Nanofibrous Scaffolds

Deng

Wei

Zhang

et al. 2015

Interface Oral Health Science 2014

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“…Human dermal fibroblasts (HDF) were successfully cultured on both sides of a thin PCL/gelatin nanofiber scaffold, which allowed cell growth and proliferation from both sides of the scaffold, thus maximizing cell loading into the stereo-structure, which mimics the natural ECM. Zhao et al prepared biodegradable core-shell fibrous scaffolds, with PCL as the core structure and gelatin forming the coating of the fibers, by a coaxial electrospinning technique [14]. The mechanical strength of the hydrated, glutaraldehyde crosslinked core-shell fibrous scaffold was significantly enhanced due to the presence of hydrophobic PCL in the core region of the fibers.…”

Section: Introductionmentioning

confidence: 99%

“…The mechanical strength of the hydrated, glutaraldehyde crosslinked core-shell fibrous scaffold was significantly enhanced due to the presence of hydrophobic PCL in the core region of the fibers. Results from cell culture studies suggested that the scaffolds were non-toxic and capable of supporting fibroblast adhesion and proliferation [14].…”

Section: Introductionmentioning

confidence: 99%

Enzymatically‐Modified Melt‐Extruded Guides for Peripheral Nerve Repair

Chiono¹,

Ciardelli²,

Vozzi³

et al. 2008

Engineering in Life Sciences

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In this work, melt-extruded guides for peripheral nerve repair were produced, based on blends between poly-(e-caprolactone) and gelatin with a low gelatin amount (10 wt. %), with the aim of combining the good melt process ability of the synthetic polymer with the optimal biocompatibility of the natural polymer. In one case, a blend was produced between poly-(e-caprolactone) and gelatin previously crosslinked by transglutaminase, using a solution mixing technique. The blend was then melt-extruded obtaining nerve guidance channels. In another case, a blend between poly-(e-caprolactone) and uncrosslinked gelatin was first produced, then melt-extruded into tube-shaped manufacts. Finally, poly-L-lysine was grafted on the gelatin domains exposed on the inner tube surface using transglutaminase catalysis, with the aim to confer to the channel guide a specific signaling for nerve cells attachment, proliferation and migration. Scanning electron microscopy (SEM) and Fourier transform infrared-attenuated total reflectance spectroscopy (FTIR-ATR) coupled with Chemical Imaging analysis showed that the obtained binary blends were poor compatible, however, appropriate mixing techniques might lead to an homogeneous distribution of gelatin domains within the poly-(e-caprolactone) matrix. Confocal microscopy (CM) was applied as a powerful tool to study the accessibility of mTGase towards gelatin substrates, using suitable model lysine-rich peptides (FITC-labeled KKKKGY). Confocal microscopy also gave a confirmation of poly-L-lysine enzymatic grafting on the exposed gelatin domains on the inner blend tube surface. In vitro cell tests using S5Y5 neuroblastoma cells showed that the produced nerve guides were biocompatible, however, gelatin was confirmed to be a non-specific protein for the attachment and proliferation of nerve cells. On the other hand, poly-L-lysine functionalization of the inner tube surface greatly improved the in-vitro cell response.

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“…18,19 Electrospinning has been employed in industrial-scale textile technologies for the preparation of nonwoven meshes, which are similar to ECM in scale. 20,21 Since the technology allows the possibility of tailoring the mechanical properties and biological properties, there has been a significant effort to adapt the technology in tissue regeneration. 22 It is the focus of this review.…”

mentioning

confidence: 99%

Next Generation of Electrosprayed Fibers for Tissue Regeneration

Hong

Madihally

2011

Tissue Engineering Part B: Reviews

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Electrospinning is a widely established polymer-processing technology that allows generation of fibers (in nanometer to micrometer size) that can be collected to form nonwoven structures. By choosing suitable process parameters and appropriate solvent systems, fiber size can be controlled. Since the technology allows the possibility of tailoring the mechanical properties and biological properties, there has been a significant effort to adapt the technology in tissue regeneration and drug delivery. This review focuses on recent developments in adapting this technology for tissue regeneration applications. In particular, different configurations of nozzles and collector plates are summarized from the view of cell seeding and distribution. Further developments in obtaining thick layers of tissues and thin layered membranes are discussed. Recent advances in porous structure spatial architecture parameters such as pore size, fiber size, fiber stiffness, and matrix turnover are summarized. In addition, possibility of developing simple three-dimensional models using electrosprayed fibers that can be utilized in routine cell culture studies is described. Tissue EngineeringT issue engineering or regeneration is a multidisciplinary study to restore, maintain, and enhance tissue and organ function. 1 In tissue engineering, biodegradable scaffolds are used to support and guide cells to proliferate, organize, and produce their own extracellular matrix (ECM). Scaffolding material eventually disappears leaving only the necessary healthy tissue in a topologically required form. 2,3 Assembly and maturation of ECM elements is important in determining the biomechanics and the quality of the tissue. For example, collagen provides tensile strength to the tissue, elastic fibers contribute to the elasticity of the tissue, while proteoglycans fills the extracellular space, creating a space for the tissue regulation of growth factors and other interactions. 4 Delicate balance between different matrix elements is necessary to generate healthy tissue. The size and shape of collagen fibers in ECM relies on tissues and organs even in the same species. The shape is cord or tape with a width of 1-20 mm and the collagen fibrils (unit can be observed by electron microscopy) are cylindrical with a diameter ranging from 10 to over 500 nm where cell is attaching and hugging. 5 Obtaining a biodegradable matrix conducive for cell colonization is a fundamental requirement for tissue regeneration. Like ECM, biomimic scaffold should allow cell attachment and migration, enables diffusion of vital cell nutrients, and retains cells. Further, chemical and mechanical properties of scaffold influence cell viability and proliferation. 6 Appropriate pore size and porosity are essential to modulate cell seeding and diffusion. Biodegradability is essential because scaffolds are absorbed and distributed to the nearby tissue, which allows no surgical removal from body. The surface of scaffold is suitable for cell attachment and migration. The mechanical properties...

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Biodegradable fibrous scaffolds composed of gelatin coated poly(ϵ‐caprolactone) prepared by coaxial electrospinning

Cited by 113 publications

References 48 publications

Biological Events Occuring on the Biosis–Abiosis Interface: Cellular Responses Induced by Implantable Electrospun Nanofibrous Scaffolds

Biological Events Occuring on the Biosis–Abiosis Interface: Cellular Responses Induced by Implantable Electrospun Nanofibrous Scaffolds

Enzymatically‐Modified Melt‐Extruded Guides for Peripheral Nerve Repair

Next Generation of Electrosprayed Fibers for Tissue Regeneration

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