Biodegradable and biomimetic elastomeric scaffolds for tissue-engineered heart valves

Xue, Yuanyuan; Sant, Vinayak; Phillippi, Julie A.; Sant, Shilpa

doi:10.1016/j.actbio.2016.10.032

Cited by 69 publications

(62 citation statements)

References 162 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The hard segments are built from diisocyanates and short chain extenders, whereas soft segments are derived from polyols [4,5]. Another interesting feature is the microphase separation of hard and soft segments that significantly influence the properties of PU [6]. Typical chain extenders in PU synthesis are low-molecular-weight diols (HO-R-OH), such as 1,4-butanediol, diethylene glycol, ethylene glycol and 1,6-hexanediol, or diamines (NH 2 -R-NH 2 ), like 1,6-hexamethylenediamine and 1,2-ethylenediamine [2,7].…”

Section: Introductionmentioning

confidence: 99%

Thermal properties of polyurethane-based composites modified with chitosan for biomedical applications

Szlachta

Ordon

Nowicka

et al. 2020

J Therm Anal Calorim

View full text Add to dashboard Cite

The thermal properties of chitosan and hydroxyapatite (HAp)-crosslinked polyurethanes (PU) prepared in a two-step bulk polymerization were investigated. Synthesis of PU was carried out using 1,6-hexamethylene diisocyanate, poly(ethylene glycol) 2000 and dibutyltin dilaurate as a catalyst. Various molar ratios of chitosan and 1,4-butanediol were applied, and the effects of incorporating different HAp amounts and the chitosan-to-BDO ratio were studied. It was found that the thermal properties of PU materials depend on polysaccharides and bioceramics load, which was confirmed by differential scanning calorimetry and thermogravimetry. The glass transition temperature increases with increasing chitosan fraction. Similarly, the onset temperature of degradation increased with chitosan addition. On the other hand, the presence of ceramics did not show a significant impact on the thermal properties of PU composites. Successful polymerization and chain extension of the isocyanate groups with hydroxyl moieties from chitosan and HAp were confirmed by Fourier transform infrared spectroscopy, and the morphology was examined using scanning electron microscopy.

show abstract

Section: Introductionmentioning

confidence: 99%

Thermal properties of polyurethane-based composites modified with chitosan for biomedical applications

Szlachta

Ordon

Nowicka

et al. 2020

J Therm Anal Calorim

View full text Add to dashboard Cite

show abstract

“…An ideal tissue-engineering material needs to have the mechanical properties similar to the natural tissue material. Compared with the native tissue regeneration process, the material should be biocompatible and biodegradable [1,2]. Synthesis of a novel biodegradable and biocompatible polyurethane material is important for biomedical engineering applications.…”

Section: Introductionmentioning

confidence: 99%

Development of Nontoxic Biodegradable Polyurethanes Based on Polyhydroxyalkanoate and L-lysine Diisocyanate with Improved Mechanical Properties as New Elastomers Scaffolds

et al. 2019

View full text Add to dashboard Cite

A nontoxic and biodegradable polyurethane was prepared, characterized, and evaluated for biomedical applications. Stretchable, biodegradable, and biocompatible polyurethanes (LPH) based on L-lysine diisocyanate (LDI) with poly(ethylene glycol) (PEG) and polyhydroxyalkanoates(PHA) of different molar ratios were synthesized. The chemical and physical characteristics of the LPH films are tunable, enabling the design of mechanically performance, hydrophilic, and biodegradable behavior. The LPH films have a Young’s modulus, tensile strength, and elongation at break in the range of 3.07–25.61 MPa, 1.01–9.49 MPa, and 102–998%, respectively. The LPH films demonstrate different responses to a change of temperature from 4 to 37 °C, with the swelling ratio for the same sample at equilibrium varying from 184% to 151%. In vitro degradation tests show the same LPH film has completely different degradation morphologies in pH of 3, 7.4, and 11 phosphate buffered solution (PBS). In vitro cell tests show feasibility that some of the LPH films are suitable for culturing rat bone marrow stem cells (rBMSCs), for future soft-tissue regeneration. The results demonstrate the feasibility of the LPH scaffolds for many biomedical applications.

show abstract

“…In order to facilitate the potential translation of CNP treatment for valve calcification therapy, we envision that the local delivery of CNPs via a tissue-engineered construct 4 or polymeric drug carrier 57 will minimize the systemic exposure and prevent the calcification of valve replacement.…”

Section: Study Limitations and Outlookmentioning

confidence: 99%

“…2 Currently, the mainstream treatment option for valve calcification is valve replacement using either mechanical valves or bioprostheses, the former requires life-long anticoagulant therapy and the latter is still unfortunately susceptible to calcification and also need re-operation. 3,4 …”

Section: Introductionmentioning

confidence: 99%

Shape-Specific Nanoceria Mitigate Oxidative Stress-Induced Calcification in Primary Human Valvular Interstitial Cell Culture

et al. 2017

Self Cite

View full text Add to dashboard Cite

Introduction Lack of effective pharmacological treatment makes valvular calcification a significant clinical problem in patients with valvular disease and bioprosthetic/mechanical valve replacement therapies. Elevated levels of reactive oxygen species (ROS) in valve tissue have been identified as a prominent hallmark and driving factor for valvular calcification. However, the therapeutic value of ROS-modulating agents for valvular calcification remains elusive. We hypothesized that ROS-modulating shape-specific cerium oxide nanoparticles (CNPs) will inhibit oxidative stress-induced valvular calcification. CNPs are a class of self-regenerative ROS-modulating agents, which can switch between Ce3+ and Ce4+ in response to oxidative microen-vironment. In this work, we developed oxidative stress-induced valve calcification model using two patient-derived stenotic valve interstitial cells (hVICs) and investigated the therapeutic effect of shape-specific CNPs to inhibit hVIC calcification. Methods Human valvular interstitial cells (hVICs) were obtained from a normal healthy donor and two patients with calcified aortic valves. hVICs were characterized for their phenotypic (mesenchymal, myofibroblast and osteoblast) marker expression by qRT-PCR and antioxidant enzymes activity before and after exposure to hydrogen peroxide (H2O2)-induced oxidative stress. Four shape-specific CNPs (sphere, short rod, long rod, and cube) were synthesized via hydrothermal or ultra-sonication method and characterized for their biocompatibility in hVICs by alamarBlue® assay, and ROS scavenging ability by DCFH-DA assay. H2O2 and inorganic phosphate (Pi) were co-administrated to induce hVIC calcification in vitro as demonstrated by Alizarin Red S staining and calcium quantification. The effect of CNPs on inhibiting H2O2-induced hVIC calcification was evaluated. Results hVICs isolated from calcified valves exhibited elevated osteoblast marker expression and decreased antioxidant enzyme activities compared to the normal hVICs. Due to the impaired antioxidant enzyme activities, acute H2O2-induced oxidative stress resulted in higher ROS levels and osteoblast marker expression in both diseased hVICs when compared to the normal hVICs. Shape-specific CNPs exhibited shape-dependent abiotic ROS scavenging ability, and excellent cytocompatibility. Rod and sphere CNPs scavenged H2O2-induced oxidative stress in hVICs in a shape- and dose-dependent manner by lowering intracellular ROS levels and osteoblast marker expression. Further, CNPs also enhanced activity of antioxidant enzymes in hVICs to combat oxidative stress. Cube CNPs were not effective ROS scavengers. The addition of H2O2 in the Pi-induced calcification model further increased calcium deposition in vitro in a time-dependent manner. Co-administration of rod CNPs with Pi and H2O2 mitigated calcification in the diseased hVICs. Conclusions We demonstrated that hVICs derived from calcified valves exhibited impaired antioxidant defense mechanisms and were more susceptible to oxidative stress th...

show abstract

Biodegradable and biomimetic elastomeric scaffolds for tissue-engineered heart valves

Cited by 69 publications

References 162 publications

Thermal properties of polyurethane-based composites modified with chitosan for biomedical applications

Thermal properties of polyurethane-based composites modified with chitosan for biomedical applications

Development of Nontoxic Biodegradable Polyurethanes Based on Polyhydroxyalkanoate and L-lysine Diisocyanate with Improved Mechanical Properties as New Elastomers Scaffolds

Shape-Specific Nanoceria Mitigate Oxidative Stress-Induced Calcification in Primary Human Valvular Interstitial Cell Culture

Contact Info

Product

Resources

About