Biodegradability of polyethylene mulching film by two Pseudomonas bacteria and their potential degradation mechanism

Hou, Lijun; Xi, Jianchao; Liu, Jiaxi; Pei-yuan, Wang; Xu, Tengqi; Liu, Tingting; Qu, Wenxing; Yan, Lin

doi:10.1016/j.chemosphere.2021.131758

Cited by 76 publications

(20 citation statements)

References 47 publications

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“…56 Notably, 7 MAGs, which were all 13 C-mPE as a solo carbon source confirmed their ability to mineralize mPE (Figure 4). Significant higher δ 13 C in the headspace of the cultures amended by both 13 C-mPE and pure isolates was observed compared to the cultures amended by the isolates only or the killed control, suggesting that the production of 13 S1), were analyzed to assess the completeness of the proposed mPE mineralization pathway 21,57,58 (Figure 5).…”

Section: ■ Resultsmentioning

confidence: 99%

“…mPE12, both are belonging to the family Mycobacteriaceae , a pangenomic analysis was performed to investigate whether mPE degradation is a common function shared by members of Mycobacteriaceae . A total of eight MAGs associated with putative mPE-degrading Mycobacteriaceae identified in this study (five Mycobacterium MAGs, two Nocardia MAGs, and one Jongsikchunia MAG), together with publicly available genomes/MAGs of Mycobacteriaceae including 35 Mycobacterium , 8 Nocardia , and 1 Jongsikchunia (Table S1), were analyzed to assess the completeness of the proposed mPE mineralization pathway ,, (Figure ).…”

Section: Resultsmentioning

confidence: 99%

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Mycobacteriaceae Mineralizes Micropolyethylene in Riverine Ecosystems

Sun

Chen

Kong

et al. 2022

Environ. Sci. Technol.

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Microplastic (MP) contamination is a serious global environmental problem. Plastic contamination has attracted extensive attention during the past decades. While physiochemical weathering may influence the properties of MPs, biodegradation by microorganisms could ultimately mineralize plastics into CO2. Compared to the well-studied marine ecosystems, the MP biodegradation process in riverine ecosystems, however, is less understood. The current study focuses on the MP biodegradation in one of the world’s most plastic contaminated rivers, Pearl River, using micropolyethylene (mPE) as a model substrate. Mineralization of 13C-labeled mPE into 13CO2 provided direct evidence of mPE biodegradation by indigenous microorganisms. Several Actinobacteriota genera were identified as putative mPE degraders. Furthermore, two Mycobacteriaceae isolates related to the putative mPE degraders, Mycobacterium sp. mPE3 and Nocardia sp. mPE12, were retrieved, and their ability to mineralize 13C-mPE into 13CO2 was confirmed. Pangenomic analysis reveals that the genes related to the proposed mPE biodegradation pathway are shared by members of Mycobacteriaceae. While both Mycobacterium and Nocardia are known for their pathogenicity, these populations on the plastisphere in this study were likely nonpathogenic as they lacked virulence factors. The current study provided direct evidence for MP mineralization by indigenous biodegraders and predicted their biodegradation pathway, which may be harnessed to improve bioremediation of MPs in urban rivers.

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Section: ■ Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Mycobacteriaceae Mineralizes Micropolyethylene in Riverine Ecosystems

Sun

Chen

Kong

et al. 2022

Environ. Sci. Technol.

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“…Polyethene is resistant to microbial breakdown because it is composed of long chains of ethylene monomers. The study of Hou et al (2022) identified a bacterium Pseudomonas knackmussii N1-2 which encodes genes for enzymes involved in PE breakdown. Sequencing of P. knackmussii N1-2 illustrates the encoding of enzymes that participate in metabolic pathways responsible for PE degradation.…”

Section: Synthetic Plastics Biodegradationmentioning

confidence: 99%

Call for biotechnological approach to degrade plastic in the era of COVID-19 pandemic

Ali

Bukhari

Rehman

2023

Saudi Journal of Biological Sciences

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“…Comamonas sp., Delftia sp., Stenotrophomonas sp., and Achromobacter sp., also figuring in some fungiculture microbiota, have been investigated for bioremediation processes. Together with lignocellulolytic fungal cultivars, these bacterial groups could compose biodegradation networks ( Mann and Wozniak, 2012 ; Tribedi and Sil, 2013 ; Wasi et al, 2013 ; Braña et al, 2016 ; Kowalczyk et al, 2016 ; Ebadi et al, 2017 ; Peixoto et al, 2017 ; Wilkes and Aristilde, 2017 ; Jacquin et al, 2019 ; Roager and Sonnenschein, 2019 ; Kučić et al, 2020 ; Roberts et al, 2020 ; Varjani et al, 2020 ; Gambarini et al, 2021 ; Lear et al, 2021 ; Cabral et al, 2022 ; Hou et al, 2022 ). To identify possible polymer degraders in these systems, samples from insect fungiculture environment could compose the initial inoculum for degradation assays, performed through interconnected culture-dependent and culture-independent workflows ( Figure 3 ; Shah et al, 2008b ; Viljakainen and Hug, 2021 ).…”

Section: What Biotechnology Could Learn From Insect Fungiculture?mentioning

confidence: 99%

Lessons From Insect Fungiculture: From Microbial Ecology to Plastics Degradation

Barcoto

Rodrigues

2022

Front. Microbiol.

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Anthropogenic activities have extensively transformed the biosphere by extracting and disposing of resources, crossing boundaries of planetary threat while causing a global crisis of waste overload. Despite fundamental differences regarding structure and recalcitrance, lignocellulose and plastic polymers share physical-chemical properties to some extent, that include carbon skeletons with similar chemical bonds, hydrophobic properties, amorphous and crystalline regions. Microbial strategies for metabolizing recalcitrant polymers have been selected and optimized through evolution, thus understanding natural processes for lignocellulose modification could aid the challenge of dealing with the recalcitrant human-made polymers spread worldwide. We propose to look for inspiration in the charismatic fungal-growing insects to understand multipartite degradation of plant polymers. Independently evolved in diverse insect lineages, fungiculture embraces passive or active fungal cultivation for food, protection, and structural purposes. We consider there is much to learn from these symbioses, in special from the community-level degradation of recalcitrant biomass and defensive metabolites. Microbial plant-degrading systems at the core of insect fungicultures could be promising candidates for degrading synthetic plastics. Here, we first compare the degradation of lignocellulose and plastic polymers, with emphasis in the overlapping microbial players and enzymatic activities between these processes. Second, we review the literature on diverse insect fungiculture systems, focusing on features that, while supporting insects’ ecology and evolution, could also be applied in biotechnological processes. Third, taking lessons from these microbial communities, we suggest multidisciplinary strategies to identify microbial degraders, degrading enzymes and pathways, as well as microbial interactions and interdependencies. Spanning from multiomics to spectroscopy, microscopy, stable isotopes probing, enrichment microcosmos, and synthetic communities, these strategies would allow for a systemic understanding of the fungiculture ecology, driving to application possibilities. Detailing how the metabolic landscape is entangled to achieve ecological success could inspire sustainable efforts for mitigating the current environmental crisis.

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Biodegradability of polyethylene mulching film by two Pseudomonas bacteria and their potential degradation mechanism

Cited by 76 publications

References 47 publications

Mycobacteriaceae Mineralizes Micropolyethylene in Riverine Ecosystems

Mycobacteriaceae Mineralizes Micropolyethylene in Riverine Ecosystems

Call for biotechnological approach to degrade plastic in the era of COVID-19 pandemic

Lessons From Insect Fungiculture: From Microbial Ecology to Plastics Degradation

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