Biodegradability of poly(tetramethylene succinate-co-tetramethylene adipate): I. Enzymatic hydrolysis

Ando, Yumiko; Yoshikawa, Katsunori; Yoshikawa, Tsutomu; Nishioka, Masaaki; Ishioka, Ryoji; Yakabe, Yoshikuni

doi:10.1016/s0141-3910(97)00140-7

Cited by 36 publications

(26 citation statements)

References 9 publications

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“…Polybutylene succinate-co-adipate (PBSA) is produced by the copolymerization of 1,4-butanediol, succinic acid, and adipic acid (4). PBSA is biologically degradable when present in composts, moist soil, fresh water with activated sludge, and seawater (1,12). Various microorganisms capable of PBSA degradation have been isolated (27,30).…”

mentioning

confidence: 99%

Novel Hydrophobic Surface Binding Protein, HsbA, Produced by Aspergillus oryzae

Ohtaki

Maéda

Takahashi

et al. 2006

Appl Environ Microbiol

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Hydrophobic surface binding protein A (HsbA) is a secreted protein (14.5 kDa) isolated from the culture broth of Aspergillus oryzae RIB40 grown in a medium containing polybutylene succinate-co-adipate (PBSA) as a sole carbon source. We purified HsbA from the culture broth and determined its N-terminal amino acid sequence. We found a DNA sequence encoding a protein whose N terminus matched that of purified HsbA in the A. ozyzae genomic sequence. We cloned the hsbA genomic DNA and cDNA from A. oryzae and constructed a recombinant A. oryzae strain highly expressing hsbA. Orthologues of HsbA were present in animal pathogenic and entomopathogenic fungi. Heterologously synthesized HsbA was purified and biochemically characterized. Although the HsbA amino acid sequence suggests that HsbA may be hydrophilic, HsbA adsorbed to hydrophobic PBSA surfaces in the presence of NaCl or CaCl 2 . When HsbA was adsorbed on the hydrophobic PBSA surfaces, it promoted PBSA degradation via the CutL1 polyesterase. CutL1 interacts directly with HsbA attached to the hydrophobic QCM electrode surface. These results suggest that when HsbA is adsorbed onto the PBSA surface, it recruits CutL1, and that when CutL1 is accumulated on the PBSA surface, it stimulates PBSA degradation. We previously reported that when the A. oryzae hydrophobin RolA is bound to PBSA surfaces, it too specifically recruits CutL1. Since HsbA is not a hydrophobin, A. oryzae may use several types of proteins to recruit lytic enzymes to the surface of hydrophobic solid materials and promote their degradation.

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mentioning

confidence: 99%

Novel Hydrophobic Surface Binding Protein, HsbA, Produced by Aspergillus oryzae

Ohtaki

Maéda

Takahashi

et al. 2006

Appl Environ Microbiol

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“…Biodegradability of the polyesters Bionolle50,51 and PLLA52,53 is well documented and recent biodegradation experiments, both in soil and ‘in vitro’ with cellulolytic bacteria, have demonstrated the biodegradability of the surface modified flax fibers used in this work 26,54. It is suggested that composites of Bionolle and PLLA with flax fibers with appropriate surface chemistry can find interesting applications as environmentally friendly composite materials for temporary applications in fields such as agriculture and constructions.…”

Section: Resultsmentioning

confidence: 73%

Biodegradable Polyesters Reinforced with Surface‐Modified Vegetable Fibers

Zini

Baiardo

Armelao

et al. 2004

Macromolecular Bioscience

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Summary: Flax fibers are investigated as reinforcing agents for biodegradable polyesters (Bionolle and poly(lactic acid) plasticized with 15 wt.‐% of acetyltributyl citrate, p‐PLLA). The composites are obtained either by high temperature compression molding fiber mats sandwiched between polymer films, or by batch mixing fibers with the molten polymer. Fibers in composites obtained by the latter method are much shorter (140–200 μm) than those of the mats (5 000 μm). Flax fibers are found to reinforce both p‐PLLA and Bionolle (i.e. tensile modulus and strength increase) when composites based on fiber mats are investigated. Conversely, analogous composites obtained by batch mixing show poor mechanical properties. The observed behavior is attributed to the combined effect of fiber length and fiber‐matrix adhesion. If flax fibers with a modified surface chemistry are used, the strength of short fiber composites is seen to improve significantly because the interface strengthens and load is more efficiently transferred. Appropriate surface modifications are performed by heterogeneous acylation reactions or by grafting poly(ethylene glycol) chains (PEG, molecular weight 350 and 750). The highest tensile strength of p‐PLLA composites is reached when PEG‐grafted flax fibers are used, whereas in the case of Bionolle the best performance is observed with acylated fibers.SEM micrograph of the fracture surface (from side to side) of a freeze fractured composite sheet of A‐p‐PLLA with A2 fibers.imageSEM micrograph of the fracture surface (from side to side) of a freeze fractured composite sheet of A‐p‐PLLA with A2 fibers.

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“…[11][12][13][14][15][16][17][18] The enzymatic degradation of other poly(adipates) were analyzed in detail by Gan et al [12] The degradation behavior depended on the crystallinity, the crystal size and the crystal structure, which influenced the degradation rate and resulting morphological changes. The occurrence of sheaf-like structures and holes on the surface after treating the polymer film with enzymatic solutions corresponds to the crystal structure of the films, which is, in turn, highly dependent on the crystallization temperature and tempering conditions.…”

Section: Enzymatic Degradationmentioning

confidence: 99%

Synthesis and Enzymatic Degradation of Soft Aliphatic Polyesters

Buchholz

Agarwal

Greiner

2015

Macromolecular Bioscience

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Novel aliphatic enzymatically degradable polyesters with short alkyl side chains for tuning crystallinity are presented in this work. The intrinsic problem of aliphatic polyesters is their brittleness and tendency to crystallize. This was modulated by the synthesis of random copolyesters based on aliphatic linear monomers, adipic acid, 1,5-pentanediol and monomers with aliphatic branches, such as 2-butyl-2-ethyl-1,3-propanediol by polycondensation. The resulting copolyesters were crystalline, wax-like or had liquid texture with varied mechanical properties and enzymatic degradability depending upon the copolymer composition. Such polyesters are of significant interest for a wide range of possible applications such as controlled drug delivery, agricultural applications and as packing materials.

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Biodegradability of poly(tetramethylene succinate-co-tetramethylene adipate): I. Enzymatic hydrolysis

Cited by 36 publications

References 9 publications

Novel Hydrophobic Surface Binding Protein, HsbA, Produced by Aspergillus oryzae

Novel Hydrophobic Surface Binding Protein, HsbA, Produced by Aspergillus oryzae

Biodegradable Polyesters Reinforced with Surface‐Modified Vegetable Fibers

Synthesis and Enzymatic Degradation of Soft Aliphatic Polyesters

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