Bioconversion of xylose to xylonic acid via co-immobilized dehydrogenases for conjunct cofactor regeneration

Bachosz, Karolina; Synoradzki, K.; Staszak, Maciej; Pinelo, Manuel; Meyer, Anne S.; Zdarta, Jakub; Jesionowski, Teofil

doi:10.1016/j.bioorg.2019.01.043

Cited by 16 publications

(11 citation statements)

References 53 publications

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“…A significant effect of the optimal co-immobilized enzymes ratio for effective catalytic action was also observed in our previous study. We found that a co-immobilized xylose dehydrogenase to alcohol dehydrogenase ratio equal to 2:1 was the most effective for the simultaneous conversion of xylose and cofactor regeneration [27]. However, in this study, a GDH:XDH ratio equal to 1:5 was selected as the most suitable and was used in the further steps of the investigation.…”

Section: Discussionmentioning

confidence: 99%

Co-Immobilization of Glucose Dehydrogenase and Xylose Dehydrogenase as a New Approach for Simultaneous Production of Gluconic and Xylonic Acid

et al. 2019

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The conversion of biomass components catalyzed via immobilized enzymes is a promising way of obtaining valuable compounds with high efficiency under mild conditions. However, simultaneous transformation of glucose and xylose into gluconic acid and xylonic acid, respectively, is an overlooked research area. Therefore, in this work we have undertaken a study focused on the co-immobilization of glucose dehydrogenase (GDH, EC 1.1.1.118) and xylose dehydrogenase (XDH, EC 1.1.1.175) using mesoporous Santa Barbara Amorphous silica (SBA 15) for the simultaneous production of gluconic acid and xylonic acid. The effective co-immobilization of enzymes onto the surface and into the pores of the silica support was confirmed. A GDH:XDH ratio equal to 1:5 was the most suitable for the conversion of xylose and glucose, as the reaction yield reached over 90% for both monosaccharides after 45 min of the process. Upon co-immobilization, reaction yields exceeding 80% were noticed over wide pH (7–9) and temperature (40–60 °C) ranges. Additionally, the co-immobilized GDH and XDH exhibited a significant enhancement of their thermal, chemical and storage stability. Furthermore, the co-immobilized enzymes are characterized by good reusability, as they facilitated the reaction yields by over 80%, even after 5 consecutive reaction steps.

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Section: Discussionmentioning

confidence: 99%

Co-Immobilization of Glucose Dehydrogenase and Xylose Dehydrogenase as a New Approach for Simultaneous Production of Gluconic and Xylonic Acid

et al. 2019

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“…TiO 2 nanoparticles adsorption GDH/PDOR/GDHt Conversion of glycerol to 1,3-propanediol [53] Amberlite-XAD7 adsorption LDH/GlDH Reductive amination of α-ketoglutarate [54] Silica gel adsorption SDR/G-DH Reduction of ethyl benzoylformate to ethyl (S)-mandelate [55] Silica-coated magnetite nanoparticle adsorption XADH/ADH Oxidation of xylose to xylonic acid [56,57] Cellulose membrane adsorption FDH/FaldDH/ADH Methanol synthesis from CO 2 [58] Anioinic macroporous poly)methyl methacrilate particles (ReliSorb SP400)…”

Section: Matrix Immobilization Methods Enzymes Applicationsmentioning

confidence: 99%

“…Using the dual-enzyme catalyst, the reaction could be performed in 1.5 h with 72% conversion and >99% enantiomeric excess. The dual-enzyme catalyst showed improved thermostability in comparison to the free enzymes and could retain 60% of its activity after being reused 30 times at 55 • C. Similarly, co-immobilization of xylose dehydrogenase and an ADH for cofactor regeneration on magnetite-silica core-shell nanoparticles has been reported and employed in the obtaining of xylonic acid from xylose [56,57]. In comparison to the free enzymes, the dual-enzyme catalyst was found to be more effective in a broader range of temperature (15-35 • C) and pH (6)(7)(8).…”

Section: Thiol-disulphide Interchangementioning

confidence: 99%

Multicatalytic Hybrid Materials for Biocatalytic and Chemoenzymatic Cascades—Strategies for Multicatalyst (Enzyme) Co-Immobilization

et al. 2021

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During recent decades, the use of enzymes or chemoenzymatic cascades for organic chemistry has gained much importance in fundamental and industrial research. Moreover, several enzymatic and chemoenzymatic reactions have also served in green and sustainable manufacturing processes especially in fine chemicals, pharmaceutical, and flavor/fragrance industries. Unfortunately, only a few processes have been applied at industrial scale because of the low stabilities of enzymes along with the problematic processes of their recovery and reuse. Immobilization and co-immobilization offer an ideal solution to these problems. This review gives an overview of all the pathways for enzyme immobilization and their use in integrated enzymatic and chemoenzymatic processes in cascade or in a one-pot concomitant execution. We place emphasis on the factors that must be considered to understand the process of immobilization. A better understanding of this fundamental process is an essential tool not only in the choice of the best route of immobilization but also in the understanding of their catalytic activity.

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“…Hence, an alternative method with mild reaction conditions, improved energy efficiency, and decreased environmental problems are needed. Biocatalysis is also an alternative non-toxic route to make the desired product under milder reaction conditions; however, slow reaction rates and delicate process control are vital for an excellent yield [21][22][23]. Meanwhile, organic acids have low corrosion impact and are reasonably selective considering the pro- Recently, the kinetics of xylose dehydration have been discussed applying heterogeneous catalysts [25,26] and homogenous catalysts, including mineral acids [27] and organic acids [12].…”

Section: Introductionmentioning

confidence: 99%

Catalytic Conversion of Xylose to Furfural by p-Toluenesulfonic Acid (pTSA) and Chlorides: Process Optimization and Kinetic Modeling

et al. 2021

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Furfural is one of the most promising precursor chemicals with an extended range of downstream derivatives. In this work, conversion of xylose to produce furfural was performed by employing p-toluenesulfonic acid (pTSA) as a catalyst in DMSO medium at moderate temperature and atmospheric pressure. The production process was optimized based on kinetic modeling of xylose conversion to furfural alongwith simultaneous formation of humin from xylose and furfural. The synergetic effects of organic acids and Lewis acids were investigated. Results showed that the catalyst pTSA-CrCl3·6H2O was a promising combined catalyst due to the high furfural yield (53.10%) at a moderate temperature of 120 °C. Observed kinetic modeling illustrated that the condensation of furfural in the DMSO solvent medium actually could be neglected. The established model was found to be satisfactory and could be well applied for process simulation and optimization with adequate accuracy. The estimated values of activation energies for xylose dehydration, condensation of xylose, and furfural to humin were 81.80, 66.50, and 93.02 kJ/mol, respectively.

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Bioconversion of xylose to xylonic acid via co-immobilized dehydrogenases for conjunct cofactor regeneration

Cited by 16 publications

References 53 publications

Co-Immobilization of Glucose Dehydrogenase and Xylose Dehydrogenase as a New Approach for Simultaneous Production of Gluconic and Xylonic Acid

Co-Immobilization of Glucose Dehydrogenase and Xylose Dehydrogenase as a New Approach for Simultaneous Production of Gluconic and Xylonic Acid

Multicatalytic Hybrid Materials for Biocatalytic and Chemoenzymatic Cascades—Strategies for Multicatalyst (Enzyme) Co-Immobilization

Catalytic Conversion of Xylose to Furfural by p-Toluenesulfonic Acid (pTSA) and Chlorides: Process Optimization and Kinetic Modeling

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