Bioconversion of volatile fatty acids from macroalgae fermentation into microbial lipids by oleaginous yeast

Xu, Xu; Kim, Ji Young; Cho, Hyun Uk; Park, Hye Rim; Park, Jong

doi:10.1016/j.cej.2014.12.011

Cited by 75 publications

(41 citation statements)

References 38 publications

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“…The utilization of different single VFAs by yeasts would be interactively influenced in a mixed VFA system. Some researchers reported that different types of acids could be used simultaneously [19], while some also found yeasts would preferentially utilize acetic acid followed by propionic and butyric acid [36]. It was found in our previous study [17] that the utilization of different acids by Y. lipolytica in a mixed VFA system occurs in a step-wise manner, not in a synchronized manner.…”

Section: Andmentioning

confidence: 83%

“…In previous studies, at appropriate culture conditions, < 2 g/L lipid concentration and 10-40% lipid content were commonly obtained in batch cultivation by using low concentrations (2-10 g/L) of VFAs as a carbon source [17]. To promote the yield of microbial lipids from VFAs, researchers also explored different cultivation modes, such as fed-batch [15,18], repeated batch [19], sequencing batch [1,20], continuous cultivation [21], and two-staged cultivation [16,22]. However, the lipid yields in these studies are unsatisfactory because of the low VFA concentrations.…”

Section: Introductionmentioning

confidence: 99%

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Enhanced lipid production by Yarrowia lipolytica cultured with synthetic and waste-derived high-content volatile fatty acids under alkaline conditions

Gao

Zhou

et al. 2020

Biotechnol Biofuels

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Background: Volatile fatty acids (VFAs) can be effective and promising alternate carbon sources for microbial lipid production by a few oleaginous yeasts. However, the severe inhibitory effect of high-content (> 10 g/L) VFAs on these yeasts has impeded the production of high lipid yields and their large-scale application. Slightly acidic conditions have been commonly adopted because they have been considered favorable to oleaginous yeast cultivation. However, the acidic pH environment further aggravates this inhibition because VFAs appear largely in an undissociated form under this condition. Alkaline conditions likely alleviate the severe inhibition of high-content VFAs by significantly increasing the dissociation degree of VFAs. This hypothesis should be verified through a systematic research. Results: The combined effects of high acetic acid concentrations and alkaline conditions on VFA utilization, cell growth, and lipid accumulation of Yarrowia lipolytica were systematically investigated through batch cultures of Y. lipolytica by using high concentrations (30-110 g/L) of acetic acid as a carbon source at an initial pH ranging from 6 to 10. An initial pH of 8 was determined as optimal. The highest biomass and lipid production (37.14 and 10.11 g/L) were obtained with 70 g/L acetic acid, whereas cultures with > 70 g/L acetic acid had decreased biomass and lipid yield due to excessive anion accumulation. Feasibilities on high-content propionic acid, butyric acid, and mixed VFAs were compared and evaluated. Results indicated that Y X/S and Y L/S of cultures on butyric acid (0.570, 0.144) were comparable with those on acetic acid (0.578, 0.160) under alkaline conditions. The performance on propionic acid was much inferior to that on other acids. Mixed VFAs were more beneficial to fast adaptation and lipid production than single types of VFA. Furthermore, cultures on food waste (FW) and fruit and vegetable waste (FVW) fermentate were carried out and lipid production was effectively improved under this alkaline condition. The highest biomass and lipid production on FW fermentate reached 14.65 g/L (Y X/S : 0.414) and 3.20 g/L (Y L/S : 0.091) with a lipid content of 21.86%, respectively. By comparison, the highest biomass and lipid production on FVW fermentate were 11.84 g/L (Y X/S : 0.534) and 3.08 g/L (Y L/S : 0.139), respectively, with a lipid content of 26.02%. Conclusions: This study assumed and verified that alkaline conditions (optimal pH 8) could effectively alleviate the lethal effect of high-content VFA on Y. lipolytica and significantly improve biomass and lipid production. These results could provide a new cultivation strategy to achieve simple utilizations of high-content VFAs and increase lipid

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Section: Andmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Enhanced lipid production by Yarrowia lipolytica cultured with synthetic and waste-derived high-content volatile fatty acids under alkaline conditions

Gao

Zhou

et al. 2020

Biotechnol Biofuels

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“…However, J. Liu et al () reported higher biomass and lipid yields (1.6 and 0.4 g/g C, respectively) from 5 g/L of butyric acid than from acetic or propionic acids in C. curvatus . Regarding VFA preferences of yeasts, researchers reported the preferential utilization of acetic acid in C. curvatus (Vajpeyi & Chandran, ), while some others demonstrated the simultaneous utilization of different acids (Xu, Kim, Cho, Park, & Park, ). On the other hand, a higher lipid content and yield in C. albidus was promoted by predominance of acetic acid (Fei et al, ).…”

Section: Oleaginous Microorganisms For Oil Productionmentioning

confidence: 99%

“…Another strategy to enhance VFA conversion efficiencies is the use of a sequential batch, where the medium is replaced when the substrate is finished resulting in an increase in biomass and lipid content (Huang et al, ; J. Liu et al, ; Xu et al, ). For instance, compared with batch cultures, an increase in lipid content in C. curvatus from 50.4% to 61% (wt/wt) was obtained after 2 cycles of culture (Xu et al, ). Huang et al () compared a sequencing batch with two‐stage batch strategies for R. toruloides on a VFA substrate concluding that the sequencing batch was the optimal strategy in terms of lipid productivity (0.57 vs. 0.26 g·L −1 ·d −1 ).…”

Section: Oleaginous Microorganisms For Oil Productionmentioning

confidence: 99%

“…These authors showed an increase in lipid yields from 0.125 to 0.27 g/g when using C. albidus in two stage batch culture with 9 g VFAs/L. Another strategy to enhance VFA conversion efficiencies is the use of a sequential batch, where the medium is replaced when the substrate is finished resulting in an increase in biomass and lipid content Xu et al, 2015). For instance, compared with batch cultures, an increase in lipid content in C. curvatus from 50.4% to 61% (wt/wt) was obtained after 2 cycles of culture .…”

Section: Operational Modementioning

confidence: 99%

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Volatile fatty acids as novel building blocks for oil‐based chemistry via oleaginous yeast fermentation

Llamas

Magdalena

González‐Fernández

et al. 2019

Biotech & Bioengineering

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Microbial oils are proposed as a suitable alternative to petroleum‐based chemistry in terms of environmental preservation. These oils have traditionally been studied using sugar‐based feedstock, which implies high costs, substrate limitation, and high contamination risks. In this sense, low‐cost carbon sources such as volatile fatty acids (VFAs) are envisaged as promising building blocks for lipid biosynthesis to produce oil‐based bioproducts. VFAs can be generated from a wide variety of organic wastes through anaerobic digestion and further converted into lipids by oleaginous yeasts (OYs) in a fermentation process. These microorganisms can accumulate in the form of lipid bodies, lipids of up to 60% wt/wt of their biomass. In this context, OY is a promising biotechnological tool for biofuel and bioproduct generation using low‐cost VFA media as substrates. This review covers recent advances in microbial oil production from VFAs. Production of VFAs via anaerobic digestion processes and the involved metabolic pathways are reviewed. The main challenges as well as recent approaches for lipid overproduction are also discussed.

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Microbial Lipid Production from Volatile Fatty Acids by Oleaginous Yeast

Park

Kim

Chang

2018

Emerging Areas in Bioengineering

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Bioconversion of volatile fatty acids from macroalgae fermentation into microbial lipids by oleaginous yeast

Cited by 75 publications

References 38 publications

Enhanced lipid production by Yarrowia lipolytica cultured with synthetic and waste-derived high-content volatile fatty acids under alkaline conditions

Enhanced lipid production by Yarrowia lipolytica cultured with synthetic and waste-derived high-content volatile fatty acids under alkaline conditions

Volatile fatty acids as novel building blocks for oil‐based chemistry via oleaginous yeast fermentation

Microbial Lipid Production from Volatile Fatty Acids by Oleaginous Yeast

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