Bioconversion of Sugarcane Molasses into Bioplastic (Polyhydroxybutyrate) using <i>Bacillus cereus</i> 2156 under Statistically Optimized Culture Conditions

Suryawanshi, Sachin R.; Sarje, Sushant S.; Loni, Prakash C.; bhujbal, Sourabh; Kamble, Prajakta P.

doi:10.1080/22297928.2020.1746197

Cited by 21 publications

(13 citation statements)

References 41 publications

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“…In addition, the strain PSR-2 could produce a maximum of 12.4 g/L of PHB using an alkali-pretreated spent mushroom substrate of sugarcane bagasse [ 83 ]. B. cereus 2156 produced 2.2 g/L of PHB using sugarcane molasses [ 84 ]. Bacillus sp.…”

Section: Resultsmentioning

confidence: 99%

Two-Stage Polyhydroxyalkanoates (PHA) Production from Cheese Whey Using Acetobacter pasteurianus C1 and Bacillus sp. CYR1

et al. 2021

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Cheese whey (CW) can be an excellent carbon source for polyhydroxyalkanoates (PHA)-producing bacteria. Most studies have used CW, which contains high amounts of lactose, however, there are no reports using raw CW, which has a relatively low amount of lactose. Therefore, in the present study, PHA production was evaluated in a two-stage process using the CW that contains low amounts of lactose. In first stage, the carbon source existing in CW was converted into acetic acid using the bacteria, Acetobacter pasteurianus C1, which was isolated from food waste. In the second stage, acetic acid produced in the first stage was converted into PHA using the bacteria, Bacillus sp. CYR-1. Under the condition of without the pretreatment of CW, acetic acid produced from CW was diluted at different folds and used for the production of PHA. Strain CYR-1 incubated with 10-fold diluted CW containing 5.7 g/L of acetic acid showed the higher PHA production (240.6 mg/L), whereas strain CYR-1 incubated with four-fold diluted CW containing 12.3 g/L of acetic acid showed 126 mg/L of PHA. After removing the excess protein present in CW, PHA production was further enhanced by 3.26 times (411 mg/L) at a four-fold dilution containing 11.3 g/L of acetic acid. Based on Fourier transform infrared spectroscopy (FT-IR), and 1H and 13C nuclear magnetic resonance (NMR) analyses, it was confirmed that the PHA produced from the two-stage process is poly-β-hydroxybutyrate (PHB). All bands appearing in the FT-IR spectrum and the chemical shifts of NMR nearly matched with those of standard PHB. Based on these studies, we concluded that a two-stage process using Acetobacter pasteurianus C1 and Bacillus sp. CYR-1 would be applicable for the production of PHB using CW containing a low amount of lactose.

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Section: Resultsmentioning

confidence: 99%

Two-Stage Polyhydroxyalkanoates (PHA) Production from Cheese Whey Using Acetobacter pasteurianus C1 and Bacillus sp. CYR1

et al. 2021

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“…The most common PHA is polyhydroxybutyrate (PHB), produced from low-cost sugarcane molasses by Bacillus cereus (Suryawanshi et al 2020) or Staphylococcus epidermidis (Sarkar et al 2014), cheap agro-residues by Bacillus sp. (Getachew and Woldesenbet 2016), date syrup by Pseudodonghicola xiamenensis (Mostafa et al 2020), non-food sugars from oil palm frond (Zahari et al 2015) or biodiesel industry by-products (García 2013) or used cooking oil (Martino 2014) by Cupriavidus necator, wheat straw lignocellulosic hydrolysates by Burkholderia sacchari (Cesário et al 2014), wheat bran hydrolysate by Ralstonia eutropha (Annamalai and Sivakumar 2016), bakery waste hydrolysate by Halomonas boliviensis (Pleissner 2014).…”

Section: Organic Waste Sourcesmentioning

confidence: 99%

Bioplastic from Renewable Biomass: A Facile Solution for a Greener Environment

et al. 2021

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Environmental pollutions are increasing day by day due to more plastic application. The plastic material is going in our food chain as well as the environment employing microplastic and other plastic-based contaminants. From this point, bio-based plastic research is taking attention for a sustainable and greener environment with a lower footprint on the environment. This evaluation should be made considering the whole life cycle assessment of the proposed technologies to make a whole range of biomaterials. Bio-based and biodegradable bioplastics can have similar features as conventional plastics while providing extra returns because of their low carbon footprint as long as additional features in waste management, like composting. Interest in competitive biodegradable materials is growing to limit environmental pollution and waste management problems. Bioplastics are defined as plastics deriving from biological sources and formed from renewable feedstocks or by a variation of microbes, owing to the ability to reduce the environmental effect. The research and development in this field of bio-renewable resources can seriously lead to the adoption of a low-carbon economy in medical, packaging, structural and automotive engineering, just to mention a few. This review aims to give a clear insight into the research, application opportunities, sourcing and sustainability, and environmental footprint of bioplastics production and various applications. Bioplastics are manufactured from polysaccharides, mainly starch-based, proteins, and other alternative carbon sources, such as algae or even wastewater treatment byproducts. The most known bioplastic today is thermoplastic starch, mainly as a result of enzymatic bioreactions. In this work, the main applications of bioplastics are accounted. One of them being food applications, where bioplastics seem to meet the food industry concerns about many the packaging-related issues and appear to play an important part for the whole food industry sustainability, helping to maintain high-quality standards throughout the whole production and transport steps, translating into cleaner and smarter delivery chains and waste management. High perspectives resides in agricultural and medical applications, while the number of fields of applications grows constantly, for example, structural engineering and electrical applications. As an example, bio-composites, even from vegetable oil sources, have been developed as fibers with biodegradable features and are constantly under research.

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“…Reddy et al (2018) have previously reported a similar observation of mutual interaction between these two variables, where the maximum production of P(3HB) (7.82 g/l) by A. nosocomialis RR20 was attained with 28 g/l of molasses and 3.2 g/l of ammonium sulfate. Moreover, Suryawanshi et al (2020) indicated that the carbon (molasses) and nitrogen (urea) sources were the most critical control factors influencing the production of P(3HB) by B. cereus 2156. On the contrary, the interactive effect between molasses, ammonium sulfate, and initial pH was found insignificant and failed to affect PHA production by B. cereus VIT-SSR1 (Evangeline and Srid- Each experimental value represents the mean of three replicates ± SD; the coded levels of the independent variables were calculated as follows:…”

Section: Interactive Effect Of Independent Variablesmentioning

confidence: 99%

“…molasses, ammonium sulfate, and initial pH, with the maximum production of P(3HB) accounting for 40.3% DCW achieved from B. cereus strain VIT-SSR1 (Evangeline and Sridharan, 2019). Recently, Suryawanshi et al (2020) also used BBD for enhancing the production of P(3HB) (59.30% DCW) by B. cereus 2156, using molasses and urea as carbon and nitrogen sources, respectively. Therefore, it is apparent that the production of PHA (85.2% DCW) by the endophytic isolate DCW -dry cell weight, P(3HB-co-3HV) -poly(3-hydroxybutyrate-co-3-hydroxyvalerate), P(3HB-co-3HHx) -poly(3-hydroxybutyrate-co-3-hydroxyhexanoate), P(3HO-co-3HD) -poly(3-hydroxyoctanoate-co-3-hydroxydecanoate), P(3HB-co-LA) -poly(3-hydroxybutyrate-co-lactate)…”

Section: Validation Of the Statistical Modelmentioning

confidence: 99%

Enhanced production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer by endophytic Bacillus cereus RCL 02 utilizing sugarcane molasses as sole source of carbon: a statistical optimization approach

Das¹,

Pal²,

Paul³

2022

bta

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Polymers of biological origin have become a topic of interest due to growing concerns about the environmental impact of the disposal of plastics. In recent years, the production of ecobenign microbial polymer polyhydroxyalkanoates (PHAs) using inexpensive and renewable resources has gained significant interest as these compounds are highly biodegradable, biocompatible, and sustainable. This study used leaf endophytic isolate Bacillus cereus RCL 02, obtained from the oil-yielding plant Ricinus communis L., to achieve statistical optimization of culture variables for the enhanced production of PHAs utilizing sugarcane molasses as the sole carbon source. A threelevel and four-factor Box-Behnken design of response surface methodology was implemented to optimize the process variables, namely molasses (carbon substrate), ammonium sulfate (nitrogen source), initial pH, and incubation period, for improved biomass formation and PHA production. The highest growth (14.8 g/l) and PHA production (85.2%, dry cell weight) by the isolate were observed with 47 g/l molasses, 3 g/l ammonium sulfate, an initial pH of 6.7, and 62 h of incubation. Statistical optimization of the process allowed achieving a 1.6-fold increase in the PHA yield (7.8-12.6 g/l) compared with the conventional single-factor system of analysis. The biopolymer thus produced was confirmed as a copolymer of 3-hydroxybutyrate and 3-hydroxyvalerate [P(3HB-co-3HV)] using 1 H nuclear magnetic resonance spectroscopic analysis and was found to contain 7.8 mol% 3-hydroxyvalerate. These findings clearly indicate the efficacy of the B. cereus RCL 02 isolate in the biotransformation of raw sugarcane molasses to P(3HV-co-3HV), without the need for supplementation with high-cost precursors.

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Bioconversion of Sugarcane Molasses into Bioplastic (Polyhydroxybutyrate) using Bacillus cereus 2156 under Statistically Optimized Culture Conditions

Cited by 21 publications

References 41 publications

Two-Stage Polyhydroxyalkanoates (PHA) Production from Cheese Whey Using Acetobacter pasteurianus C1 and Bacillus sp. CYR1

Two-Stage Polyhydroxyalkanoates (PHA) Production from Cheese Whey Using Acetobacter pasteurianus C1 and Bacillus sp. CYR1

Bioplastic from Renewable Biomass: A Facile Solution for a Greener Environment

Enhanced production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer by endophytic Bacillus cereus RCL 02 utilizing sugarcane molasses as sole source of carbon: a statistical optimization approach

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