Bioconversion of steroids by Cochliobolus lunatus. I. Transformation of Reichstein's compound S with cell‐free preparations of Cochliobolus lunatus

Abstract

Steroid 11/3-hydroxylase activity and 20B-hydroxy-steroid dehydrogenase activity were found in the cell-free crude extract of Cochliobolus lunatus. It was possible to separate these two enzymatic activities by centrifugation at 100,000 x g. The 20B-hydroxy-steroid dehydrogenase activity remained in the supernatant while the ll/I-hydroxylase activity was present in the sediment. 11s-hydroxylation was clearly inhibited by CO.

Biotransformation enzymes in Cunninghamella blakesleeana (NCIM‐687)

“…The presence of camphor induced mixed function oxygenase has been reported in soil bacterium Pseudomonas putida and induced by barbiturate in Bacillus megaterium (DUS et al 1984, BLACK et al 1994. Steroid 11-β hydroxylase is found as inducible enzyme in Curvularia lunata and Chochliobolus lunatus (SUZUKI et al 1993, HÖRHOLD et al 1986).…”

Study of mixed function oxidase system in Aspergillus ochraceus (NCIM 1146)

“…The presence of camphor induced mixed function oxygenase has been reported in soil bacterium Pseudomonas putida 23 and induced by barbiturate in Bacillus megaterium 24 , steroid 11-β-hydroxylase is also found in fungi, Cochliobolus lunatus and Curvularia lunata as inducible enzyme [25][26] . The transfer of cells of Candida maltosa from a glucose medium to an n-alkane medium had resulted in the induction of various enzymes such as cytochrome P-450 (P-450 alk ) and dramatically increases the endoplasmic reticulum and peroxisomes 27 .…”