Bioconversion of residual glycerol from biodiesel synthesis into 1,3-propanediol and ethanol by isolated bacteria from environmental consortia

Rossi, Daniele Misturini; Costa, Janaína Berne da; Souza, Elisangela Aquino de; Peralba, Maria do Carmo Ruaro; Ayub, Marco Antônio Záchia

doi:10.1016/j.renene.2011.08.005

Cited by 71 publications

(46 citation statements)

References 22 publications

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“…One of the main products obtained through microbial bioconversion of glycerol is 1,3-propanediol (1,3-PDO), a polyol with applications in the cosmetics, food, lubricant, and pharmaceutical industries [18][19][20][21]. The development of polypropylene terephthalate (PPT), a thermoplastic with superior physicochemical properties to those of polyethylene terephthalate (PET), used in the production of fabrics, carpets, and engineered plastics has created a new demand for 1,3-propanediol.…”

Section: Introductionmentioning

confidence: 99%

Production and productivity of 1,3-propanediol from glycerol by Klebsiella pneumoniae GLC29

2015

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a b s t r a c tInterest in the development of the bioproduction of 1,3-propanediol, an important chemical intermediate with various industrial applications, has increased in recent years. Klebsiella pneumoniae is one of the most studied and efficient bacteria for 1,3-propanediol production from glycerol. A new isolate of K. pneumoniae was investigated using response surface methodology by central composite design for the production of 1,3-propanediol using glycerol. The effects of pH, temperature, stirrer speed, and glycerol concentration on the production and productivity of 1,3-propanediol were examined. Considering both production and productivity, the best conditions for glycerol conversion in 1,3-propanediol are: a pH range of 6.9-7.1, a temperature between 33 and 38.5 • C, a stirrer speed of 110-180 rpm, and a glycerol concentration of 39-49 g l −1 . Batch fermentation carried out at a pH of 7.0, a temperature of 35 • C, a stirrer speed of 150 rpm, and a glycerol concentration of 40 g l −1 produced 20.4 g 1,3-propanediol l −1 , with a maximum volumetric productivity of 2.92 g l −1 h −1 and a yield of 0.51 g g −1 . The main byproducts were acetic acid (approximately 7.0 g l −1 ) and formate (approximately 3.7 g l −1 ). The newly isolated K. pneumoniae GLC29 showed potential for the conversion of glycerol into 1,3-propanediol, with high production and productivity.

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Section: Introductionmentioning

confidence: 99%

Production and productivity of 1,3-propanediol from glycerol by Klebsiella pneumoniae GLC29

2015

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“…oxytoca U14, K. pneumoniae PLB, and S. marcescens M25C were more effective for glycerol degradation, showing 100 % degradation at the end of the experiment. Numerous studies have reported bacteria of the genus Klebsiella using residual glycerol as a (Mu et al 2006;Zeng and Sabra 2011;Rodriguez et al 2012;Rossi et al 2012).…”

Section: Resultsmentioning

confidence: 99%

Detoxification of Mercury by Bacteria Using Crude Glycerol from Biodiesel as a Carbon Source

Giovanella

Costa

Schaffer

et al. 2015

Water Air Soil Pollut

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Bacteria that harbor the mer operon in their genome are able to enzymatically reduce mercury (II) to the volatile form of mercury Hg (0). Detoxification of contaminated waste by using these bacteria may be an alternative to conventional methods for mercury removal. Residual glycerol from the biodiesel industry can be used as a carbon source to accelerate the process. This work shows for the first time the feasibility of using residual glycerol as a carbon source for Hg removal by bacteria prospected from contaminated environments. Eight bacterial isolates were able to remove mercury and degrade glycerol in mineral medium and residual glycerol. Mercury removal was monitored by atomic absorption spectroscopy and glycerol degradation by high performance liquid chromatography. The best results of mercury removal and glycerol degradation were obtained using isolates of Serratia marcescens M25C (85 and 100 %), Klebsiella pneumoniae PLB (90 and 100 %), Klebsiella oxytoca (90 and 100 %), and Arthrobacter sp. U3 (80 and 75 %), with addition of 0.5 g L −1 yeast extract. The Arthrobacter sp. U3 isolate is common in soils and has proven to be a promising candidate for environment applications due to its low pathogenicity and higher Hg removal and glycerol degradation rates.

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“…Recently, the discovery of a bacterial strain producing both 1,3-PDO and ethanol from crude glycerol has been published (Rossi et al [2012]). In order to reach high 1,3-PDO titers, the concentration of the second product, ethanol will also increase and possibly inhibit the organism.…”

Section: Discussionmentioning

confidence: 99%

Production of 1,3-PDO and butanol by a mutant strain of Clostridium pasteurianum with increased tolerance towards crude glycerol

Jensen

Kvist²,

Mikkelsen³

et al. 2012

AMB Expr

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The production of biodiesel results in a concomitant production of crude glycerol (10% w/w). Clostridium pasteurianum can utilize glycerol as sole carbon source and converts it into 1,3-propanediol, ethanol, butanol, and CO2. Reduced growth and productivities on crude glycerol as compared to technical grade glycerol have previously been observed. In this study, we applied random mutagenesis mediated by ethane methyl sulfonate (EMS) to develop a mutant strain of C. pasteurianum tolerating high concentrations of crude glycerol. At an initial crude glycerol concentration of 25 g/l the amount of dry cell mass produced by the mutant strain was six times higher than the amount produced by the wild type. Growth of the mutant strain was even detected at an initial crude glycerol concentration of 105 g/l. A pH controlled reactor with in situ removal of butanol by gas-stripping was used to evaluate the performance of the mutant strain. Utilizing stored crude glycerol, the mutant strain showed significantly increased rates compared to the wild type. A maximum glycerol utilization rate of 7.59 g/l/h was observed along with productivities of 1.80 g/l/h and 1.21 g/l/h of butanol and 1,3-PDO, respectively. These rates are higher than what previously has been published for C. pasteurianum growing on technical grade glycerol in fed batch reactors. In addition, high yields of the main products (butanol and 1,3-PDO) were detected and these two products were efficiently separated in two steams using gas-stripping.

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Bioconversion of residual glycerol from biodiesel synthesis into 1,3-propanediol and ethanol by isolated bacteria from environmental consortia

Cited by 71 publications

References 22 publications

Production and productivity of 1,3-propanediol from glycerol by Klebsiella pneumoniae GLC29

Production and productivity of 1,3-propanediol from glycerol by Klebsiella pneumoniae GLC29

Detoxification of Mercury by Bacteria Using Crude Glycerol from Biodiesel as a Carbon Source

Production of 1,3-PDO and butanol by a mutant strain of Clostridium pasteurianum with increased tolerance towards crude glycerol

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