Bioconversion of pimelic acid into biotin by Bacillus sphaericus bioB transformants

Ohsawa, Ikuroh; Kisou, T.; Kodama, Kazuko; Yoneda, Ikuhiro; Speck, Denis; Gloeckler, R.; Lemoine, Yves; Kamogawa, K.

doi:10.1016/0922-338x(92)90475-a

Cited by 9 publications

(6 citation statements)

References 19 publications

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“…20) In these strains, however, high expression of the biotin operon resulted in growth inhibition. Biotin overproduction has also been reported by other research groups in genetically engineered E. coli, 21,22) Bacillus sphaericus, 23,24) and Serratia marcescens, 25,26) and some of them have also observed growth inhibition 22 ,26) and plasmid instability23) due to overexpression of the biotin operon.…”

mentioning

confidence: 87%

Molecular Analysis of Growth Inhibition Caused by Overexpression of the Biotin Operon inEscherichia coli

Ifuku¹,

Koga²,

Haze³

et al. 1995

Bioscience, Biotechnology, and Biochemistry

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mentioning

confidence: 87%

Molecular Analysis of Growth Inhibition Caused by Overexpression of the Biotin Operon inEscherichia coli

Ifuku¹,

Koga²,

Haze³

et al. 1995

Bioscience, Biotechnology, and Biochemistry

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“…Derivatives of PA can be used in lysine biosynthesis. It is also required for the synthesis of biotin (vitamin B7), which is important in fatty acid synthesis, branched-chain amino acid catabolism, and gluconeogenesis. − …”

Section: Introductionmentioning

confidence: 99%

“…HP − Dissociated pimelic acid[HP] o Initial concentration of pimelic acid, mol/kg [HP] aq Pimelic acid concentration in aqueous phase, mol/kg HP ex Undissociated acid reacted with the extractant [HP] org Pimelic acid concentration in organic phase, mol/kg H 2 P Undissociated pimelic acid K a Acid dissociation constant, m 3 /kmol K D Distribution coefficient K E Equilibrium complexation constant, (kg/mol) or (kg/ mol) 0.5 m Acid:amine mole ratio in the complex structure P − Dissociated pimelic acid PA Pimelic acid R 2 Correlation coefficient R 2 adj Adjusted correlation coefficient RMSE Root mean square error TOA Tri-n-octylamine [TOA] org TOA concentration in organic phase, mol/kg z Loading ratio η Viscosity, mPa•s ρ Density, g/cm3 …”

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confidence: 99%

Separation of Pimelic Acid Using Tri-n-octylamine in Various Organic Solvents by Reactive Extraction: Equilibrium and Modeling

2021

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Separation of carboxylic acids from dilute aqueous solutions is a challenging problem, and reactive extraction has been examined as an attractive alternative for this process. This study aims to investigate the reactive extraction of pimelic acid (PA) using tri-n-octylamine (TOA) in 1-hexanol, 1-octanol, xylene, and n-hexane. The highest extraction efficiency (E (%)) and distribution coefficient (K D ) were obtained at pH 3, which is the unadjusted pH of the aqueous PA solution. Extractive efficiency varied inversely with temperature and initial PA concentration but increased with the increase in TOA amount. The highest E (%) and K D values (98.5% and 67.2, respectively) were obtained with 1-hexanol at initial TOA and PA concentrations of 0.2700 and 0.0375 mol/kg, respectively. The process was modeled using multilinear regression, and the model closely correlated with experimental results for all organic solvents tested.

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“…Recombinant strains of E. coli (6,25), Bacillus sphaericus (11,19), and Serratia marcescens (26) that overproduce biotin were constructed previously by various groups interested in alternatives to the chemical synthesis of this vitamin. The R. meliloti strains constructed here represent the first attempt to use biotin synthesis genes to manipulate plant-microbe interactions.…”

Section: Discussionmentioning

confidence: 99%

Recombinant Rhizobium meliloti strains with extra biotin synthesis capability

Streit

Phillips

1996

Appl Environ Microbiol

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The growth of Rhizobium meliloti 1021 in an experimental alfalfa (Medicago sativa L.) rhizosphere was stimulated by adding nanomolar amounts of biotin. To overcome this biotin limitation, R. meliloti strains were constructed by conjugating the Escherichia coli biotin synthesis operon into biotin auxotroph R. meliloti 1021-B3. Transconjugant strains Rm1021-WS10 and Rm1021-WS11 grew faster in vitro and achieved a higher cell density than did R. meliloti 1021 and overproduced biotin on a defined medium. The increase in cell yield was associated with as much as a 99% loss in viability for Rm1021-WS11, but data suggested that a separate stabilizing factor in the E. coli DNA reduced cell death in Rm1021-WS10. In rhizosphere tests, the recombinant strains showed delayed growth and competed poorly against Rm1021.

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Bioconversion of pimelic acid into biotin by Bacillus sphaericus bioB transformants

Cited by 9 publications

References 19 publications

Molecular Analysis of Growth Inhibition Caused by Overexpression of the Biotin Operon inEscherichia coli

Molecular Analysis of Growth Inhibition Caused by Overexpression of the Biotin Operon inEscherichia coli

Separation of Pimelic Acid Using Tri-n-octylamine in Various Organic Solvents by Reactive Extraction: Equilibrium and Modeling

Recombinant Rhizobium meliloti strains with extra biotin synthesis capability

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