Bioconversion of Isoeugenol Into Vanillin by Crude Enzyme Extracted From Soybean

Abstract

Crude enzyme extracted from soybean was used to convert isoeugenol into vanillin. The effects of several factors on the bioconversion were studied. Conversion was affected by the amount of substrate and was also improved by the addition of absorbents, among which powdered activated carbon was the best. The effect of H2O2 concentration on the conversion was also studied. The optimum concentration of H2O2 was 1% (v/v). With 10 g/L of powdered activated carbon and 0.1% H2O2 added, vanillin reached a maximum concentration of 2.46 g/L after 36 h, corresponding to a molar yield of 13.3%.

Biotransformation of Isoeugenol to Vanillin by a Novel Strain of Bacillus fusiformis

“…The biotransformation of isoeugenol to vanillin using a crude enzyme from soybean has been investigated by our research group (Li et al 2004(Li et al , 2005. In this study, the biotransformation of isoeugenol to vanillin ( Figure 1) by a new isolate of Bacillus fusiformis is described.…”

Enzymatic synthesis of vanillin and related catalytic mechanism

“…This result, obtained by simple enzymatic catalysis with the crude enzyme preparation, without the addition of any other additives, is higher than the yield (2.46 g L –1 ) obtained by Li et al . with the same enzyme but under the optimal conditions that they had worked out, especially with the addition of H 2 O 2 (as a co‐oxidant) and activated carbon (as an absorbent for the product), both of which have been found to be effective in enhancing the production yield . The conversion obtained in this study is also higher than that obtained using E. coli cells harbouring the decarboxylase/oxygenase cascade (1.2 g L –1 ), and is more than three times as high as that obtained by whole‐cell catalysis with Lysinibacillus fusiformis CGMCC1347 cells as the catalyst (0.7 g L –1 ) …”

“…were the first to report the ability of lipoxygenase (LOX) to act as a catalyst for converting isoeugenol to vanillin: they carried out a preliminary study on the effect of two factors (i.e. substrate concentration and the addition of activated carbon powders as product absorbents and H 2 O 2 as a co‐oxidant) on the enzymatic reaction . In 2017, Furuya et al .…”

Biotechnology of Vanillin: Vanillin from Microbial Sources

“…The use of styrene dioxygenase, vanillyl alcohol oxidase (dehydrogenase), and lipoxygenase have been reported (van den Heuvel et al 2004;Li et al 2005). The use of styrene dioxygenase, vanillyl alcohol oxidase (dehydrogenase), and lipoxygenase have been reported (van den Heuvel et al 2004;Li et al 2005).…”