Bioconversion of Beet Molasses to Alpha-Galactosidase and Ethanol

Álvarez-Cao, María-Efigenia; Cerdán, M. Esperanza; González-Siso, María-Isabel; Becerra, Manuel

doi:10.3389/fmicb.2019.00405

Cited by 26 publications

(26 citation statements)

References 40 publications

(49 reference statements)

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“…There are several types of biomass that can be used to make ethanol, including sugar cane, corn, and their residual substances; molasses from land plants, and marine biomass from seaweeds. Bioethanol is considered a renewable source of energy because biomasses are permanent resources that can be continuously produced and renewed [1] [2].…”

Section: Introductionmentioning

confidence: 99%

Bioethanol Production from Molasses by Yeasts with Stress-Tolerance Isolated from Aquatic Environments in Japan

Naito¹,

Okai²,

Ishida³

et al. 2019

AiM

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Bioethanol is a safe and renewable source of energy that continues to be a research focus, since fossil fuels have been linked to global warming and nuclear energy sources are affected by the increased safety concerns following the 2011 nuclear power plant accident in Japan. In general, bioethanol is converted from a biomass by yeast fermentation. The production efficiency of this bioethanol is not sufficiently high, and its practical use as a substitute for fossil fuels and nuclear energy is thus limited. For the industrial production of bioethanol, the yeast fermentation of biomass cultures containing high concentration sugar, NaCl, and ethanol is necessary, but this might induce phenomena in which the stresses arising in the yeasts weaken their cells during fermentation. As described herein, we isolated 1028 strains of yeasts from natural aquatic environments: Japan's Tama River and Lake Kasumigaura. Among them, 412 strains were fermentative yeasts and 31 strains showed high fermentation ability under a 30% sorbitol + 10% ethanol condition. These strains were identified as Torulaspola delbrueckii, Wickerhamomyces anomalus, Candida glabrata, Pichia kudriavzevii, Saccharomyces cf. cerevisiae/paradoxus, and Lachancea kluyveri. The strains T. delbrueckii, W. anomalus, and C. glabrata also showed tolerance against 15% NaCl. Most importantly, S. cf. cerevisiae/paradoxus H28 and L. kluyveri F2-67 produced 57.4 g/L and 53.9 g/L ethanol from molasses (sucrose 104.0 g/L, fructose 33.4 g/L, and glucose 24.8 g/L) within 48 hrs at 25˚C, respectively.

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Section: Introductionmentioning

confidence: 99%

Bioethanol Production from Molasses by Yeasts with Stress-Tolerance Isolated from Aquatic Environments in Japan

Naito¹,

Okai²,

Ishida³

et al. 2019

AiM

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“…Interestingly, this strain could produce γ-PGA in a glutamate independent manner. The aim of our study was to use GJ11 for microbial conversion of FA powder into γ-PGA with low cost, and investigate whether γ-PGA could be used as an activator to trigger ISR in plants [25]. We found that FA powder could reduce the cost of γ-PGA production around one third, and the γ-PGA produced by GJ11 could trigger ISR against pathogens infection via the ethylene signaling and NPR1 in plants.…”

Section: Pgamentioning

confidence: 99%

Bioconversion of poly-γ-glutamic acid (γ-PGA) from fulvic acid powder produced from the wastewater of yeast molasses fermentation

Li¹,

Wang²,

Ke³

et al. 2020

Preprint

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Background: Molasses is a wildly used feedstock for fermentation, but it poses a severe wastewater-disposal problem worldwide. Recently, the wastewater produced by yeast during molasses fermentation is being processed into fulvic acid (FA) powder as a fertilizer for crops, but it consequently induces a problem of soil acidification after being directly applied in soil. In this study, the low-cost FA powder was bioconverted into a value-added product, γ-PGA, by a glutamate independent producer, Bacillus velezensis GJ11. Results: With FA powder, the substrates of sodium glutamate and citrate sodium used in medium were decreased around one third. Moreover, FA powder could completely substitute Mg2+, Mn2+, Ca2+ and Fe3+ in the fermentation medium. In the optimized FA powder fermentation medium, the γ-PGA was produced with its maximum concentration at 42.55 g/L and a productivity of 1.15 g/(L·h), while only 2.87 g/L was produced in the medium without FA powder. Hydrolyzed γ-PGA could trigger induced systemic resistance (ISR), e.g. H2O2 accumulation and callose deposition, against the pathogen infection in plants. Further investigations found that the ISR triggered by γ-PGA hydrolyzates was dependent on the ethylene signalling and NPR1. Conclusions: To our knowledge, this is the first report of using the industry waste, FA powder, as a sustainable substrate for the microbial synthesis of γ-PGA. This bioprocess can not only develop a new way of FA powder as a cheap feedstock for producing γ-PGA, but also help to reduce pollution from the wastewater of yeast molasses fermentation.

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“…Interestingly, this strain could also produce γ-PGA in a glutamate-independent manner. The aim of this study was to use GJ11 for microbial conversion of FA powder into γ-PGA with low cost, and investigate whether γ-PGA could be used as an activator to trigger ISR in plants [ 25 ]. We found that FA powder could reduce the cost of γ-PGA production around one-third, and the γ-PGA produced by GJ11 was able to trigger ISR against pathogens infection via the ethylene (ET) signaling and nonexpressor of pathogenesis-related proteins 1 (NPR1) in plants.…”

Section: Introductionmentioning

confidence: 99%

Microbial synthesis of poly-γ-glutamic acid (γ-PGA) with fulvic acid powder, the waste from yeast molasses fermentation

Wang

Liu

et al. 2020

Biotechnol Biofuels

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Background Molasses is a wildly used feedstock for fermentation, but it also poses a severe wastewater-disposal problem worldwide. Recently, the wastewater from yeast molasses fermentation is being processed into fulvic acid (FA) powder as a fertilizer for crops, but it consequently induces a problem of soil acidification after being directly applied into soil. In this study, the low-cost FA powder was bioconverted into a value-added product of γ-PGA by a glutamate-independent producer of Bacillus velezensis GJ11. Results FA power could partially substitute the high-cost substrates such as sodium glutamate and citrate sodium for producing γ-PGA. With FA powder in the fermentation medium, the amount of sodium glutamate and citrate sodium used for producing γ-PGA were both decreased around one-third. Moreover, FA powder could completely substitute Mg2+, Mn2+, Ca2+, and Fe3+ in the fermentation medium for producing γ-PGA. In the optimized medium with FA powder, the γ-PGA was produced at 42.55 g/L with a productivity of 1.15 g/(L·h), while only 2.87 g/L was produced in the medium without FA powder. Hydrolyzed γ-PGA could trigger induced systemic resistance (ISR), e.g., H2O2 accumulation and callose deposition, against the pathogen’s infection in plants. Further investigations found that the ISR triggered by γ-PGA hydrolysates was dependent on the ethylene (ET) signaling and nonexpressor of pathogenesis-related proteins 1 (NPR1). Conclusions To our knowledge, this is the first report to use the industry waste, FA powder, as a sustainable substrate for microbial synthesis of γ-PGA. This bioprocess can not only develop a new way to use FA powder as a cheap feedstock for producing γ-PGA, but also help to reduce pollution from the wastewater of yeast molasses fermentation.

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Bioconversion of Beet Molasses to Alpha-Galactosidase and Ethanol

Cited by 26 publications

References 40 publications

Bioethanol Production from Molasses by Yeasts with Stress-Tolerance Isolated from Aquatic Environments in Japan

Bioethanol Production from Molasses by Yeasts with Stress-Tolerance Isolated from Aquatic Environments in Japan

Bioconversion of poly-γ-glutamic acid (γ-PGA) from fulvic acid powder produced from the wastewater of yeast molasses fermentation

Microbial synthesis of poly-γ-glutamic acid (γ-PGA) with fulvic acid powder, the waste from yeast molasses fermentation

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