Bioconversion of amino acids into flavouring alcohols and esters by Erwinia carotovora subsp. atroseptica

Spinnler, Henry-Éric; Djian, Aleth

doi:10.1007/bf00184699

Cited by 16 publications

(10 citation statements)

References 11 publications

(7 reference statements)

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“…Thus, our findings make a significant contribution to this developing area of research in plant defense mechanisms. This is the first report of TSL as a metabolite from the bacterium E. cloacae, although other bacterial species, including Bacillus lactis aerogenes, Escherichia coli, Proteus vulgaris, and Erwinia carotovora [17,49,50,51] have been previously reported to produce TSL from tyrosine. This is also the first report of the antifungal activity of TSL against G. pulicaris, the causative pathogen of Fusarium dry rot.…”

Section: Discussionmentioning

confidence: 96%

Antifungal and sprout regulatory bioactivities of phenylacetic acid, indole-3-acetic acid, and tyrosol isolated from the potato dry rot suppressive bacterium Enterobacter cloacae S11:T:07

Slininger

Burkhead

Schisler

2004

J IND MICROBIOL BIOTECHNOL

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Enterobacter cloacae S11: T:07 (NRRL B-21050) is a promising biological control agent that has significantly reduced both fungal dry rot disease and sprouting in laboratory and pilot potato storages. The metabolites phenylacetic acid (PAA), indole-3-acetic acid (IAA), and tyrosol (TSL) were isolated from S11:T:07 liquid cultures provided with three different growth media. The bioactivities of these metabolites were investigated via thin-layer chromatography bioautography of antifungal activity, wounded potato assays of dry rot suppressiveness, and cored potato eye assays of sprout inhibition. Relative accumulations of PAA, IAA, and TSL in cultures were nutrient dependent. For the first time, IAA, TSL, and PAA were shown to have antifungal activity against the dry rot causative pathogen Gibberella pulicaris, and to suppress dry rot infection of wounded potatoes. Disease suppression was optimal when all three metabolites were applied in combination. Dosages of IAA that resulted in disease suppression also resulted in sprout inhibition. These results suggest the potential for designing culture production and formulation conditions to achieve a dual purpose biological control agent able to suppress both dry rot and sprouting of stored potatoes.

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Section: Discussionmentioning

confidence: 96%

Antifungal and sprout regulatory bioactivities of phenylacetic acid, indole-3-acetic acid, and tyrosol isolated from the potato dry rot suppressive bacterium Enterobacter cloacae S11:T:07

Slininger

Burkhead

Schisler

2004

J IND MICROBIOL BIOTECHNOL

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“…and Erwinia carotovora. [19][20][21] Further studies regarding bacterial 2-PE production involve metabolic engineering strategies. 22,23 The 2-PE production process is dependent on the producing strain, but the medium composition and cultivation conditions also play an important role in production.…”

Section: Introductionmentioning

confidence: 99%

Optimal conditions for 2‐phenylethanol production from l‐phenylalanine by Acinetobacter soli ANG344B

Bernardino,

Torres,

Grosso

et al. 2024

J of Chemical Tech & Biotech

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BACKGROUND2‐Phenylethanol (2‐PE) is a valuable flavor compound renowned for its rose‐like scent and widespread application in the cosmetic, perfume and food industries. While it can be naturally sourced from plants, the majority is currently produced through chemical synthesis, leading to environmental concerns. As an ecofriendly alternative, biotechnological production of 2‐PE presents a promising solution, yielding a natural product.RESULTSIn this work, the newly isolated bacterium Acinetobacter soli ANG344B was used to produce 2‐PE from l‐phenylalanine (L‐Phe), using glucose as carbon source, aiming to determine the best conditions for cellular growth and aroma production. The optimal conditions for Acinetobacter cultivation and 2‐PE production were 30 °C and pH 7. Further, yeast extract proved to be a key element for 2‐PE production. The concentration of L‐Phe that maximizes 2‐PE volumetric productivity was found to be 5 g L−1, present in the cultivation media from the beginning of the cultivation. Under these conditions 2.14 ± 0.18 g L−1 of the aroma compound was produced, which is the highest concentration obtained for a wild‐type bacterium. The toxicity of 2‐PE was also evaluated, revealing that cellular growth of A. soli ANG344B was adversely affected at concentrations above 1 g L−1, and beyond 4 g L−1 no cellular growth was observed.CONCLUSIONBy elucidating the crucial factors affecting 2‐PE production by A. soli ANG344B, we pave the way for the development of biotechnological strategies to harness its potential as a natural and sustainable aroma compound. © 2024 Society of Chemical Industry (SCI).

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“…The branched chain fusel alcohols include isobutanol, 3-methyl butanol (isoamyl alcohol) and 2-methyl butanol while 2-phenylethanol is the most well-known and used aromatic fusel alcohol. Both branched chain and aromatic fusel alcohols and acetates can be produced artificially through chemical synthesis or naturally by plants and microorganisms (Spinnler & Djian, 1991, Goettmann et al, 2006, Hazelwood et al, 2008. Since chemical synthesis is environmentally unfriendly, natural production of branched chain and aromatic fusel alcohols and acetates is more sustainable (Etschmann et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Catabolism of branched chain and aromatic amino transferases, route to fusel alcohols and acetates by the Ceratocystidaceae.

Mailula

Nest

Hammerbacher

et al. 2020

Proceedings of 1st International Electronic Conference on Microbiology

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Fungi in the family Ceratocystidaceae produce fusel alcohols and acetates, which have fruity and floral odours. These compounds are valuable additives of food products, perfumes and soaps. Our aim was to analyse the volatiles produced by the Ceratocystidaceae and to study their biosynthesis. All the strains included in our study produced high levels of isoamyl acetate while some members of Bretziella, Berkeleyomyces, Ceratocystis and Huntiella produced isobutyl acetate. The majority of Ceratocystidaceae also produced 2-phenylethyl acetate in low quantities. Fusel alcohols are produced in fungi from amino acids via the Ehrlich pathway in a three-step catabolic process. We searched in the available genomes of fungi in the Ceratocystidaceae for genes encoding enzymes involved in catalysing the first and second steps in the production of the fusel alcohols. We discovered three gene sequences encoding putative aromatic amino transferases, three gene sequences encoding putative branched-chain amino transferases and a single gene sequence encoding a putative pyruvate decarboxylase in each of the genomes. We also discovered that most fungi of the Sordariomycetes which do not produce fruity or floral smells have a similar number of copies of each of these genes. The amino transferases from C. albifundus, C. manginecans, E. polonica and H. monilliformis were heterologously expressed and functionally characterized by in vitro enzyme assays. All the assayed enzymes were either functional aromatic amino transferases accepting the substrates phenylalanine, tyrosine and tryptophan or branched-chain amino acid transferases accepting the substrates leucine, isoleucine or valine.

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Bioconversion of amino acids into flavouring alcohols and esters by Erwinia carotovora subsp. atroseptica

Cited by 16 publications

References 11 publications

Antifungal and sprout regulatory bioactivities of phenylacetic acid, indole-3-acetic acid, and tyrosol isolated from the potato dry rot suppressive bacterium Enterobacter cloacae S11:T:07

Antifungal and sprout regulatory bioactivities of phenylacetic acid, indole-3-acetic acid, and tyrosol isolated from the potato dry rot suppressive bacterium Enterobacter cloacae S11:T:07

Optimal conditions for 2‐phenylethanol production from l‐phenylalanine by Acinetobacter soli ANG344B

Catabolism of branched chain and aromatic amino transferases, route to fusel alcohols and acetates by the Ceratocystidaceae.

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