Bioconversion of Agroindustrial Waste in the Production of Bioemulsifier by Stenotrophomonas maltophilia UCP 1601 and Application in Bioremediation Process

Abstract

This study investigated the potential of the bacterium Stenotrophomonas maltophilia UCP 1601 to produce a new biomolecule with emulsifying properties by determining the hemolytic activity, obtaining a halo of 9 mm in blood agar. Fermentations were carried out in saline mineral medium supplemented with 10% waste soybean oil (WSO) and different concentrations of glucose, peptone, ZnCl2, and MgSO4, according to a 24 full-factorial design. The results showed that the best results were obtained in condition 6 (medium composed of 4% glucose, 1% peptone, 2.72% ZnCl2, and 2.46% MgSO4), with excellent high emulsification index of 82.74%, using burned motor oil. The emulsifying property of the biomolecule produced was confirmed by the emulsification index of 78.57, 54.07, and 58.62%, using soybean, corn, and diesel oils, respectively, and the stability at different values of pH, temperature, and NaCl concentrations. The yield of the produced bioemulsifier was 2.8 g/L, presenting an anionic character and polymeric nature (37.6% lipids, 28.2% proteins, and 14.7% carbohydrates), confirmed by FTIR. The new bioemulsifier demonstrated promising potential for bioremediation of hydrophobic contaminants in the environment, since it had the ability to reduce the viscosity of WSO and burned motor oil, as well as excellent dispersion capacity of the burned motor oil in water (69.94 cm2 of oil displacement area), and removing 71.7% of this petroleum derivative from sandy soil.

Potential Food Application of a Biosurfactant Produced by Saccharomyces cerevisiae URM 6670

“…Figure 1 shows possible carbonyl groups (C=O) and single carbon bonds (C-C) corresponding to the respective stretches between 1500 and 2000 cm −1 as well as between 2700 and 3000 cm −1 . Carbon double bonding (C=C) was also found in the region of approximately 1465 cm −1 , as described by Nogueira et al (2020) for the bioemulsifier from Stenotrophomonas maltophilia UCP 1601. A small stretching region was found in the 3000-3500 cm −1 range, indicating the presence of hydroxyl (OH) groups in the molecule.…”

Management of Cattle Dung and Novel Bioelectricity Generation Using Microbial Fuel Cells: An Ingenious Experimental Approach

“…is has demonstrated the scope for bioremediation and manufacturing of anionic and polymeric bioemulsifiers with promising dispersing characteristics. A new biomolecule was utilized and prepared from UCP 1601 with emulsifying behaviour [15]. Another study [16] reported the use of pomegranate peel that acts as bioadsorbents for wastewater treatment.…”

“…Mostly, a review of advanced research referred in [10][11][12][13][14][15][16][17] indicates a research gap that no comprehensive performance analysis of MFCs with cattle dung as a substrate for a wide range management of cattle waste and the effect of different electrodes like popularly used carbon and novel metals on the performance of MFCs have been done. In addition, the scientometric analysis has been conducted based on keywords generated through the Scopus database.…”

A Novel Mass Transfer Model to Describe the Separation Process in Reverse Osmosis of Glucose

“…e field of fermentation technology has rapidly advanced over recent years [1]. In the microbial fermentation process, the concentration of fermentable sugar is a key component that directly impacts the ultimate concentration of target products [2].…”