Bioconversion of Agave Bagasse to Produce Cellulases and Xylanases by Penicillium citrinum and Aspergillus fumigatus in Solid-State Fermentation

Valle-Pérez, Alexander U.; Flores-Cosío, G.; Amaya-Delgado, Lorena

doi:10.1007/s12649-021-01397-y

Cited by 13 publications

(4 citation statements)

References 42 publications

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“…Aspergillus spp. has high xylanase activity, such as Aspergillus sulphureus JCM01963, Aspergillus fumigatus and so on [40,41]. In KEGG annotation, many genes are assigned to carbohydrate metabolism.…”

Section: Discussionmentioning

confidence: 99%

Safety Evaluation and Whole Genome Sequencing of Aspergillus japonicas PJ01 Reveal Its Potential to Degrade Citrus Segments in Juice Processing

Qian

Gao

Wang

et al. 2021

Foods

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Aspergillus japonicas PJ01 (A. japonicas PJ01) is a strain isolated from the rotten branches. In previ-ous studies, it was shown that it can produce complex enzymes to degrade polysaccharide com-ponents. In this study, we evaluated the safety of its crude enzyme solution. Acute oral toxicity, subchronic toxicity, micronucleus and sperm malformation tests all validated the high biologi-cal safety for the crude enzymes. Secondly, we carried out the citrus segment degradation ex-periment of crude enzyme solution. Compared with the control group, the crude enzyme solu-tion of A. japonicas PJ01 can completely degrade the segments in 50 min, which provides the basis for enzymatic peeling during juice processing. The whole genome sequencing showed that the genome of A. japonicus PJ01 has a GC content of 51.37% with a size of 36204647 bp, and encoded 10070 genes. GO, COG, KEGG and CAZy databases were used in gene annotation analyses. Pathway enrichment showed many genes related to carbohydrate metabolism, rich in genes re-lated to pectinase, xylanase and carboxylcellulase. Therefore, the complex enzyme produced by A. japonicus PJ01 can be used in gizzard juice processing to achieve efficient enzymatic decapsu-lation.

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Section: Discussionmentioning

confidence: 99%

Safety Evaluation and Whole Genome Sequencing of Aspergillus japonicas PJ01 Reveal Its Potential to Degrade Citrus Segments in Juice Processing

Qian

Gao

Wang

et al. 2021

Foods

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“…The response surface methodology (RSM) was applied to evaluate the effect of enhancing the developed ink with hydroxyapatite and calcium carbonate over the structural similarity index from the printed structure. According to the statistical modeling reported in recent studies[ 15 , 16 ], the elaboration of the RSM was performed with the MATLAB ® software, and the model’s constants were obtained with the Minitab 18 software (Minitab® LLC, USA). The general model is presented in Equation 1 , and the simplified resulting model is shown in Equation 2 .…”

Section: Methodsmentioning

confidence: 99%

Ecologically Friendly Biofunctional Ink for Reconstruction of Rigid Living Systems Under Wet Conditions

Ávila-Ramírez

Valle-Pérez

Susapto

et al. 2024

IJB

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The development of three-dimensional (3D)-printable inks is essential for several applications, from industrial manufacturing to novel applications for biomedical engineering. Remarkably, biomaterials for tissue engineering applications can be expanded to other new horizons; for instance, restoration of rigid living systems as coral reefs is an emergent need derived from recent issues from climate change. The coral reefs have been endangered, which can be observed in the increasing bleaching around the world. Very few studies report eco-friendly inks for matter since most conventional approaches require synthetic polymer, which at some point could be a pollutant depending on the material. Therefore, there is an unmet need for cost-effective formulations from eco-friendly materials for 3D manufacturing to develop carbonate-based inks for coral reef restoration. Our value proposition derives from technologies developed for regenerative medicine, commonly applied for human tissues like bone and cartilage. In our case, we created a novel biomaterial formulation from biopolymers such as gelatin methacrylate, poly (ethylene glycol diacrylate), alginate, and gelatin as scaffold and binder for the calcium carbonate and hydroxyapatite bioceramics needed to mimic the structure of rigid structures. This project presents evidence from 2D/3D manufacturing, chemical, mechanical, and biological characterization, which supports the hypothesis of its utility to aid in the fight to counteract the coral bleaching that affects all the marine ecosystem, primarily when this is supported by solid research in biomaterials science used for living systems, it can extend tissue engineering into new approaches in different domains such as environmental or marine sciences.

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“…It is widely described that slightly acidic environments can favor xylanase action. It was shown that [41], the activity of xylanases produced by Penicillium citrinum and Aspergillus fumigatus in SSF using wheat straw and sugarcane bagasse at different pH levels and temperatures displayed an optimal pH of around 5, and the optimal temperature was around 50°C [33].…”

Section: In Uence Of Ph and Temperature On Xylanase Activity By Penic...mentioning

confidence: 99%

Production of xylanases from Penicillium sp. FSDE15 using agro-industrial residues and its biotechnological potential

Nunes,

Padilha,

Costa

et al. 2024

Preprint

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This study investigates the production and application of xylanases derived from the fungus Penicillium sp. FSDE15, obtained via solid-state fermentation (SSF). Wheat bran served as the substrate, either in its pure form or mixed with sisal fiber. Penicillium sp. underwent incubation in SSF for 168 h, with daily sampling to evaluate xylanase activity. Xylanase analyses were conducted under various temperature and pH conditions to optimize enzymatic activity, with the optimal conditions determined as 50°C and pH 4.8. SDS-PAGE electrophoresis revealed the enzyme's molecular weight as 66 kDa. Evaluation of Penicillium sp. xylanase for enzymatic hydrolysis of sugarcane straw (untreated and alkaline pretreated) and corn cob xylan showcased significant activity, with 102.34 U/g and a productivity of 1.67 U/g.h observed in 72 h, surpassing experiments with the blend of bran and sisal fiber. Electrophoresis analysis identified a single protein band at 66 kDa. Although optimal conditions were identified, the enzyme exhibited instability at alkaline pH values (> 6.0) and elevated temperatures (> 60°C). When compared to a commercial enzyme, FSDE15 demonstrated satisfactory performance in saccharification and xylooligosaccharide (XOS) production, including X2, indicating its biotechnological potential. This study underscores the efficacy of SSF in xylanase production by Penicillium sp. FSDE15 has implications for converting lignocellulosic biomass into value-added products, particularly in saccharification and XOS production.

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Bioconversion of Agave Bagasse to Produce Cellulases and Xylanases by Penicillium citrinum and Aspergillus fumigatus in Solid-State Fermentation

Cited by 13 publications

References 42 publications

Safety Evaluation and Whole Genome Sequencing of Aspergillus japonicas PJ01 Reveal Its Potential to Degrade Citrus Segments in Juice Processing

Safety Evaluation and Whole Genome Sequencing of Aspergillus japonicas PJ01 Reveal Its Potential to Degrade Citrus Segments in Juice Processing

Ecologically Friendly Biofunctional Ink for Reconstruction of Rigid Living Systems Under Wet Conditions

Production of xylanases from Penicillium sp. FSDE15 using agro-industrial residues and its biotechnological potential

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