Biocontrol potential of Pochonia chlamydosporia var. chlamydosporia isolates against Meloidogyne javanica on pistachio

Abstract

The biocontrol potential of four Pochonia chlamydosporia var. chlamydosporia isolates on the root-knot nematode, Meloidogyne javanica was investigated on pistachio plants in a greenhouse experiment. Isolates were applied at 10,000, 5000 and 1000 chlamydospores per gram of soil; the latter two densities were being used for strain Pcc60 only and designated as PccB and PccA, respectively. Plants were inoculated with a suspension containing 3000 eggs of M. javanica. Nematode reproduction was reduced to 57% by Pcc20 and 36% by Pcc10 and Pcc60C after 4 months. Pcc20 was the most promising isolate as the final egg population of nematodes was reduced by nearly 61 and 36% by Pcc10 and Pcc60C, respectively. All strains infected eggs on the roots to varying degrees. Pcc20 was the most effective colonizer of all the isolates, infecting 37% of the eggs, while Pcc10 and Pcc60C as the second best colonizers caused an average infection rate of 17.5%. The potential of the fungus was reduced at lower densities of spore inoculum. Semi-selective medium was used to re-isolate the fungi from soil and rhizosphere but was not able to detect Pcc60A at 1000 cfu/g soil, despite the fact that 3% of the eggs were parasitized. The efficiency of the different isolates in controlling nematode reproduction parameters and their relationships in terms of survival and abundance is discussed further.

Biocontrol of Bean and Wheat Aphids by Fungi Isolated from Indigenous and Invasive Insects Collected from Different Locations in Minia Governorate, Egypt

“…The biological control potential of P. chlamydosporia var. chlamydosporia against Meloidogyne javanica was also reported by Ebadi et al (2018). Extracts from cultures of Pochonia chlamydosporia was reported to induce 100% mortality of the pea aphid after 72 h of exposure (Lacatena et al 2019).…”

Biomanagement of rice root‐knot nematode Meloidogyne graminicola using five indigenous microbial isolates under pot and field trials

“…(Ebadi et al . 2018). In the present study, P. chlamydosporia parasitized all the stages of the nematode as evident from the higher frequency of occurrence of the fungus in the adult females, eggs and juveniles of M. graminicola .…”

Propriedades biológicas e químicas de solos arenoso e argiloso tratados com isolados fúngicos sob soja e milho em casa-de-vegetação

“…Pesquisas mostraram que P. chlamydosporia coloniza as raízes endofiticamente, favorecendo diretamente as plantas, pela via de sinalização do jasmonato, diminuindo o período de floração, estimulando o crescimento das plantas e melhorando tanto produção de sementes como suas defesas (EBADI et al, 2018;LIN et al, 2018). Foi observado que P. chlamydosporia consegue colonizar raízes endofiticamente de tomate (BORDALLO et al, 2002), batata (MANZANILLA-LÓPEZ et al, 2011) e cevada (MONFORT et al, 2005).…”