Biocontrol potential of fiveBurkholderiaandPseudomonasstrains againstColletotrichum truncatuminfecting chilli pepper

Sandani, H. B. P.; Ranathunge, N. P.; Lakshman, P.L.N.; Weerakoon, W. M. W.

doi:10.1080/09583157.2019.1597331

Cited by 27 publications

(7 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…completely inhibited the germination of A. alternata spores while all the other selected antagonists showed a significant inhibition greater than 50 %. These suggest the high potential of these bacterial strains being successful biological control agents on A. alternata agreeing with many scientific findings (Sandani et al, 2019;Rahman et al, 2007). Further studies must be focused on this aspect as seed borne infection, fruit rot and tomato leaf colonization of A. alternata could be conveniently controlled by such antagonistic agents or their products at the initial stage.…”

Section: Discussionsupporting

confidence: 71%

“…Thickenings and swellings of the A. alternata hyphae could be observed as a result of the inhibition inserted by the antagonist in dual culture. Such abnormalities of many other fungal hyphae have been recorded in previous studies as well (Rahman et al, 2007, Sandani et al, 2019.…”

Section: Effect Of Antagonism On Hyphal Growth and Morphologysupporting

confidence: 80%

“…There are many reports which explain the successful applications of microbial antagonists in plant disease management (Sharma et al, 2009;Zhang et al, 2007;Droby, 2006;Korsten, 2006;Zhang et al, 2005;Janisiewicz & Korsten, 2002;Droby et al, 1992;. Successful attempts of biological control of chilli (Sandani et al, 2019, Intanoo & Chamswarng, 2007and Boonratkwang et al, 2007, strawberries (Freeman et al, 2001) and citrus (Moretto et al, 2001) using microbial antagonists have been reported.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Biocontrol potential of some selected native bacterial antagonists against tomato (Solanum lycopersicum L.) early blight causal agent Alternaria alternata

Sandani,

Sithumini,

Weerahewa

2024

Ceylon J. Sci.

View full text Add to dashboard Cite

Seven selected bacterial antagonists isolated from healthy tomato rhizosphere and phyllosphere were evaluated for their biocontrol efficacy against the tomato early blight causal agent Alternaria alternata under in vitro conditions. The causal organism of early blight of tomato was isolated from a tomato leaf sample which showed early blight symptoms and verified it as Alternaria alternata. Healthy tomato rhizosphere soil, fructosphere and phyllosphere extracts were used as the potential sources of antagonists. Thirty bacterial isolates from tomato rhizosphere soil and 22 bacterial isolates from tomato phyllosphere were isolated using serial dilution technique. Out of those isolates, 24 were subjected to antagonists screening procedure. It was observed that, all the bacterial isolates except RA 5, RA 21, PA 1, PA 2, inhibited the growth of A. alternata significantly (p<0.05) in co-cultivation and dual culture showing an average radial growth inhibition of 69 %. Seven different bacterial isolates coded as RA 8, RA 12, RA 17, RA 18, RA 29, PA 4 and PA 12 were selected for further studies. Based on 16 S rRNA analysis, four of these antagonists were identified as Bacillus thuringiensis, Bacillus cereus and Pseudomonas sp. Microscopic observations of A. alternata hyphae subjected to antagonism showed deviations from the normal hyphae including thickenings, swellings and vacuolation. Inhibition of A. alternata spore germination by selected antagonists was significant at p<0.05 level with an average inhibition of 83.7 %. All the selected antagonists produced diffusible antifungal substances and showed hyperparasitism and competition as their mechanisms in antagonizing the fungal pathogen A. alternata. These results suggested that these seven bacterial isolates can be further explored as potential biocontrol agents in controlling early blight disease in tomato.

show abstract

Section: Discussionsupporting

confidence: 71%

Section: Effect Of Antagonism On Hyphal Growth and Morphologysupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Biocontrol potential of some selected native bacterial antagonists against tomato (Solanum lycopersicum L.) early blight causal agent Alternaria alternata

Sandani,

Sithumini,

Weerahewa

2024

Ceylon J. Sci.

View full text Add to dashboard Cite

show abstract

“…Some strains of Burkholderia spp. and Pseudomonas aeruginosa exhibited instead a strong efficacy for the control of C. truncatum in chili, as reported by Sandani et al [ 78 ] that revealed a reduction of anthracnose incidence ranging from 75% to 100%.…”

Section: Innovative and Sustainable Approachesmentioning

confidence: 56%

Management of Post-Harvest Anthracnose: Current Approaches and Future Perspectives

Ciofini

Negrini

Baroncelli

et al. 2022

Plants

View full text Add to dashboard Cite

Anthracnose is a severe disease caused by Colletotrichum spp. on several crop species. Fungal infections can occur both in the field and at the post-harvest stage causing severe lesions on fruits and economic losses. Physical treatments and synthetic fungicides have traditionally been the preferred means to control anthracnose adverse effects; however, the urgent need to decrease the use of toxic chemicals led to the investigation of innovative and sustainable protection techniques. Evidence for the efficacy of biological agents and vegetal derivates has been reported; however, their introduction into actual crop protection strategies requires the solutions of several critical issues. Biotechnology-based approaches have also been explored, revealing the opportunity to develop innovative and safe methods for anthracnose management through genome editing and RNA interference technologies. Nevertheless, besides the number of advantages related to their use, e.g., the putative absence of adverse effects due to their high specificity, a number of aspects remain to be clarified to enable their introduction into Integrated Pest Management (IPM) protocols against Colletotrichum spp. disease.

show abstract

“…can produce extracellular secondary metabolities such as cepacins, testin, 2,4-diacetylphloroglucinol, pyoluteorin, phenazine, pyrrolnitrin, pyoverdine and altericidines that inhibit the growth of some soilborne fungal pathogens (Mavrodi et al 2001 ; Subagio and Foster 2003 ), and are attributed to the production of siderophores, volatile compounds, cell wall-degrading molecules, extracellular chitinase, and protease enzymes activity (Mavrodi et al 2001 ). Likewise, under in vitro conditions, Sandani et al ( 2019 ) showed that Burkholderia and Pseudomonas bacteria are effective antagonists of C. truncatum causing anthracnose. Also, Ali et al ( 2018 ) studied 40 PGPR strains which are able to produce siderophores as well as have a significant growth suppression of pathogenic F. oxysporum and R. solani .…”

Section: Discussionmentioning

confidence: 99%

Siderophore production by Bacillus subtilis MF497446 and Pseudomonas koreensis MG209738 and their efficacy in controlling Cephalosporium maydis in maize plant

2020

View full text Add to dashboard Cite

Late wilt disease, caused by Cephalosporium maydis in maize plant, is one of the main economical diseases in Egypt. Therefore, to cope with this problem, we investigated the potentiality of plant growth promoting rhizobacteria in controlling this disease. Six strains (Bacillus subtilis, B. circulance, B. coagulanse, B. licheniformis, Pseudomonas fluroscence and P. koreensis) were screened for siderophore production, and using dual plate culture method and greenhouse experiment, antagonistic activity against C. maydis was studied. Using two superior strains, single and dual inoculation treatments in maize were applied in field experiment during the 2018 and 2019 seasons. Results indicated that B. subtilis and P. koreensis strains had shown the most qualitative and quantitative assays for siderophore production and antagonistic activities. In greenhouse, the most effective treatments on the pre-and post-emergence damping off as well as growth promotion of maize were T3 treatment (inoculated with B. subtilis), and T8 treatment (inoculated with P. koreensis). In field experiment, T5 treatment (inoculated with a mixture of B. subtilis and P. koreensis) showed significant increases in catalase (CAT), peroxidase (POX) and polyphenol oxidase (PPO) activities, as well as total chlorophyll and carotenoids than control treatments during the two growing seasons. In the same way, the highest effect in reducing infection and increasing the thickness of the sclerenchymatous sheath layer surrounding the vascular bundles in maize stem was observed and these results were a reflection of the increase in yield and yield parameters.

show abstract

Biocontrol potential of fiveBurkholderiaandPseudomonasstrains againstColletotrichum truncatuminfecting chilli pepper

Cited by 27 publications

References 50 publications

Biocontrol potential of some selected native bacterial antagonists against tomato (<em>Solanum lycopersicum</em> L.) early blight causal agent <em>Alternaria alternata</em>

Biocontrol potential of some selected native bacterial antagonists against tomato (<em>Solanum lycopersicum</em> L.) early blight causal agent <em>Alternaria alternata</em>

Management of Post-Harvest Anthracnose: Current Approaches and Future Perspectives

Siderophore production by Bacillus subtilis MF497446 and Pseudomonas koreensis MG209738 and their efficacy in controlling Cephalosporium maydis in maize plant

Contact Info

Product

Resources

About