Biocontrol potential of entomopathogenic nematode species against Tribolium confusum (Jac.) (Coleoptera: Tenebrionidae) and Rhyzopertha dominica (Fab.) (Coleoptera: Bostrichidae) under laboratory conditions

Abstract

Grain commodities, like cereals and legumes, are subject to insect infestation during postharvest processing and storage. Public concerns about the potential risks that derive from the consumption of pesticide-treated commodities have created interest for the development and integration in stored product protection of alternative, ecologically safe methods for the disinfestation of stored commodities. The present study was conducted to evaluate the biocontrol potential of four entomopathogenic nematodes (EPNs): Steinernema pakistanense (LM-07), S. bifurcatum (LM-30), S. affinae (GB-14), and S. cholashanense (GB-22) against adult beetles of Tribolium confusum (Jac.) (Coleoptera: Tenebrionidae) and Rhyzopertha dominica (Fab.) (Coleoptera: Bostrichidae) under laboratory conditions. Suspensions of nematodes were applied at three different concentrations, 50, 100, and 150 IJs/beetle in 1 ml of distilled water at three different temperatures, 20, 25, and 30°C. Mortality was recorded after 3 days of application. The concentration of 150 IJs/beetle achieved a maximum mortality of 100% for S. pakistanense at 30°C. The same concentrations revealed that all four species of EPN were able to cause mortal effects depended on temperature and concentrations. S. pakistanense (LM-07) and S. bifurcatum (LM-30) were the most effective at 150 IJs/beetle at 30°C and S. affinae (GB-14) and S. cholashanense (GB-22) at the same concentration at 20°C.

Pathogenicity of the Steinernema feltiae TUR-S3 (Rhabditida: Steinernematidae) Isolate on Oryzaephilus surinamensis (Coleoptera: Silvanidae) and Tribolium confusum (Coleoptera: Tenebrionidae)

“…Steinernema feltiae has been evaluated against various stored product pests, including T. confusum and Ephestia kuehniella Zeller (Lepidoptera: Pyralidae), demonstrating its insecticidal effect (Athanassiou et al, 2008;Athanassiou et al, 2010;Guru et al, 2022). Javed et al (2020) investigated the efficacy of isolates from different species of the Steinernematidae family at three different doses on adult T. confusum and Rhyzopertha dominica (Coleoptera: Bostrychidae). The results indicated an increase in the mortality rate of adults with the application of higher concentrations of IJs.…”

Natural Warriors against Stored-Grain Pests: The Joint Action of Beauveria bassiana and Steinernema carpocapsae

“…and Heterorhabditis spp. in the early 1990s, there has been growing interest in the research community, leading to increased study and investigation of these organisms [ 43 , 44 , 45 , 46 , 47 , 48 ].…”

Entomopathogenic Nematode (Nematoda: Rhabditida) Survey and their Occurrence in Soil of District Lakki Marwat, Khyber Pakhtunkhwa Province, Pakistan

“…N ematode and insects could be believed as utmost successful groups of invertebrate animals in the nature. Ahuja et al, 2020;Mokrini et al, 2020;Torrini et al, 2020;Salma et al, 2020;Dichusa et al, 2021) fall under the orders Lepidoptera, Coleoptera, and Diptera (Burnell and Stock, 2000). Essential parameters such as moisture, soil texture, potential of Hydrogen (PH) and organic matters are vital aspects that affecting the virulence, existence and perseverance of these nematodes (Sandhi et al, 2020).…”