Biocontrol potential of bacteria belonging to the Bacillus subtilis group against pests and diseases of agricultural interest through genome exploration

Rocha, Gabriela Teodoro; Queiroz, Paulo Roberto Martins; Grynberg, Priscila; Togawa, Roberto Coiti; Ferreira, Antonia Débora Camila de Lima; Nascimento, Izabela Nunes do; Gomes, A. C. M. M.; Monnerat, R. G.

doi:10.1007/s10482-023-01822-3

Cited by 4 publications

(2 citation statements)

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“…Competitive inhibition of pathogens can also occur via the direct production of antagonistic compounds by Bacillus species, including surfactin, iturin, fengycin/plipastatin, bacillomycin, and bacilysin ( Fig. 2 ; [ 94 ]), although the effectiveness of these compounds has not specifically been demonstrated in the built environment. Several studies have demonstrated that Bacillus intervention enhances the pathogen exclusion properties of the extant surface microbiome [ 95 , 96 ].…”

Section: Techniques For Investigating the Microbiome Of The Built Env...mentioning

confidence: 99%

Biocontrol in built environments to reduce pathogen exposure and infection risk

Gottel,

Hill,

Neal

et al. 2024

The ISME Journal

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The microbiome of the built environment comprises bacterial, archaeal, fungal, and viral communities associated with human-made structures. Even though most of these microbes are benign, antibiotic-resistant pathogens can colonise and emerge indoors, creating infection risk through surface transmission or inhalation. Several studies have catalogued the microbial composition and ecology in different built environment types. These have informed in vitro studies that seek to replicate the physicochemical features that promote pathogenic survival and transmission, ultimately facilitating the development and validation of intervention techniques used to reduce pathogen accumulation. Such interventions include using Bacillus-based cleaning products on surfaces or integrating bacilli into printable materials. Though this work is in its infancy, early research suggests the potential to use microbial biocontrol to reduce hospital- and home-acquired multidrug-resistant infections. Although these techniques hold promise, there is an urgent need to better understand the microbial ecology of built environments and to determine how these biocontrol solutions alter species interactions. This review covers our current understanding of microbial ecology of the built environment and proposes strategies to translate that knowledge into effective biocontrol of antibiotic-resistant pathogens.

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Section: Techniques For Investigating the Microbiome Of The Built Env...mentioning

confidence: 99%

Biocontrol in built environments to reduce pathogen exposure and infection risk

Gottel,

Hill,

Neal

et al. 2024

The ISME Journal

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“…B. amyloliquefaciens CH-2 inhibits the growth of S. sclerotiorum , but also inhibited the formation of sclerotia. When managing the disease during the crop production cycle, the main form of control is through synthetic fungicides ( Rocha et al, 2023 ). The biocontrol strain Bacillus velezensis was isolated from our laboratory.…”

Section: Introductionmentioning

confidence: 99%

Genomic and biological control of Sclerotinia sclerotiorum using an extracellular extract from Bacillus velezensis 20507

Cheng,

Lou,

et al. 2024

Front. Microbiol.

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IntroductionSclerotinia sclerotiorum is a known pathogen that harms crops and vegetables. Unfortunately, there is a lack of effective biological control measures for this pathogen. Bacillus velezensis 20507 has a strong antagonistic effect on S. Sclerotiorum; however, the biological basis of its antifungal effect is not fully understood.MethodsIn this study, the broad-spectrum antagonistic microorganisms of B. velezensis 20507 were investigated, and the active antifungal ingredients in this strain were isolated, purified, identified and thermal stability experiments were carried out to explore its antifungal mechanism.ResultsThe B. velezensis 20507 genome comprised one circular chromosome with a length of 4,043,341 bp, including 3,879 genes, 185 tandem repeats, 87 tRNAs, and 27 rRNAs. Comparative genomic analysis revealed that our sequenced strain had the closest genetic relationship with Bacillus velezensis (GenBank ID: NC 009725.2); however, there were significant differences in the positions of genes within the two genomes. It is predicted that B. velezensis 20507 encode 12 secondary metabolites, including difficidin, macrolactin H, fengycin, surfactin, bacillibactin, bacillothiazole A-N, butirosin a/b, and bacillaene. Results showed that B. velezensis 20507 produced various antagonistic effects on six plant pathogen strains: Exserohilum turcicum, Pyricularia oryzae, Fusarium graminearum, Sclerotinia sclerotiorum, Fusarium oxysporum, and Fusarium verticillioides. Acid precipitation followed by 80% methanol leaching is an effective method for isolating the antifungal component ME80 in B. velezensis 20507, which can damage the membranes of S. sclerotiorum hyphae and has good heat resistance. Using high-performance liquid chromatography, and Mass Spectrometry analysis, it is believed that fengycin C72H110N12O20 is the main active antifungal substance.DiscussionThis study provides new resources for the biological control of S. Sclerotiorum in soybeans and a theoretical basis for further clarification of the mechanism of action of B. velezensis 20507.

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