Biocontrol potential of Apilactobacillus kunkeei EIR/BG-1 against infectious diseases in honey bees (Apis mellifera L.)

Kıran, Fadime; Sevin, Sedat; Ceylan, Ahmet

doi:10.1007/s11259-022-10036-3

Cited by 4 publications

(4 citation statements)

References 69 publications

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“…This would also be an advantage to any host that stores pollen, and could help explain its presence in most of the social species in this analysis. Apilactobacillus increases individual resistance to a number of pathogens including Paenibacillus larvae (American foulbrood, Butler et al., 2013 ; Forsgren et al., 2010 ; Kačániová et al., 2020 ; Kiran et al., 2022 ), the microsporidian Nosema (Arredondo et al., 2018 ), fungal infection (Iorizzo et al., 2020 ) and Melissococcus plutonius (European foulbrood, Endo et al., 2012 ; Endo & Salminen, 2013 ; Vásquez et al., 2012 ; Zendo et al., 2020 ). It is also prevalent in the floral environment, suggesting an intuitive route for transmission between different bee species visiting the same flowers (Anderson et al., 2013 ; Tamarit et al., 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Influence of social lifestyles on host–microbe symbioses in the bees

Mee,

Barribeau

2023

Ecology and Evolution

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Microbiomes are increasingly recognised as critical for the health of an organism. In eusocial insect societies, frequent social interactions allow for high‐fidelity transmission of microbes across generations, leading to closer host–microbe coevolution. The microbial communities of bees with other social lifestyles are less studied, and few comparisons have been made between taxa that vary in social structure. To address this gap, we leveraged a cloud‐computing resource and publicly available transcriptomic data to conduct a survey of microbial diversity in bee samples from a variety of social lifestyles and taxa. We consistently recover the core microbes of well‐studied corbiculate bees, supporting this method's ability to accurately characterise microbial communities. We find that the bacterial communities of bees are influenced by host location, phylogeny and social lifestyle, although no clear effect was found for fungal or viral microbial communities. Bee genera with more complex societies tend to harbour more diverse microbes, with Wolbachia detected more commonly in solitary tribes. We present a description of the microbiota of Euglossine bees and find that they do not share the “corbiculate core” microbiome. Notably, we find that bacteria with known anti‐pathogenic properties are present across social bee genera, suggesting that symbioses that enhance host immunity are important with higher sociality. Our approach provides an inexpensive means of exploring microbiomes of a given taxa and identifying avenues for further research. These findings contribute to our understanding of the relationships between bees and their associated microbial communities, highlighting the importance of considering microbiome dynamics in investigations of bee health.

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Section: Discussionmentioning

confidence: 99%

Influence of social lifestyles on host–microbe symbioses in the bees

Mee,

Barribeau

2023

Ecology and Evolution

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“…Apilactobacillus kunkeei EIR/BG-1, isolated from the gut microbiota of honeybees (Kiran et al, 2023), and Enterococcus hirae EIR/CM-2, isolated from the microbiota of cow milk (Sevin et al, 2021), were kindly provided by the Pharmabiotic Technologies Research Laboratory at Ankara University (Turkey). Briefly, homogenised gut samples of honeybees in 1 ml phosphate buffer saline (PBS) and 1 ml cow milk samples were serially diluted 10-fold, and each dilution was pourplated on De Man Rogosa and Sharpe agar (MRS; Merck, Darmstadt, Germany) plates.…”

Section: Bacterial Strains and Growth Conditionsmentioning

confidence: 99%

“…Following the incubation at 37 °C for 48 h, bacterial colonies were randomly selected, and pure cultures were transferred to MRS broth supplemented with 50% glycerol at −80 °C for long-term storage (Arredondo et al, 2018). Species identification from colony isolates was carried out by sequencing the 16S ribosomal-RNA (16S rRNA) subunit gene using the protocol of Kiran et al (2023). Based on the sequencing results of the 16S rRNA gene region (approximately 1492 bp) and subsequent Basic Local Alignment Search Tool (BLAST) searches against the GenBank Bacteria and Archaea 16S rRNA sequences database, the isolates were found to closely match Apilactobacillus kunkeei and Enterococcus hirae (99% similarity), and were registered in the National Center for Biotechnology Information (NCBI).…”

Section: Bacterial Strains and Growth Conditionsmentioning

confidence: 99%

Preliminary study of the effects of Apilactobacillus kunkeei EIR/BG-1 and Enterococcus hirae EIR/CM-2 supplementation on in vitro rumen fermentation and microbial population

Demirtas,

Sevin,

Musa

et al. 2023

J. Anim. Feed Sci.

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“…The strains of L. curvatus Kar.9b and A. kunkeei K1.10 (nucleotide database accession numbers, MW829532.1 and MW600523.1, respectively) used in this study were isolated from the gut microbiota of honeybees (Apis mellifera) in previous studies (Kiran et al 2022). These strains were selected for their high EPS production.…”

Section: Bacterial Strains Culture Media and Growth Conditionsmentioning

confidence: 99%

Exopolysaccharides of lactic acid bacteria isolated from honeybee gut and effects of their antibiofilm activity against Streptococcus mutans

KARACA

2023

An. Acad. Bras. Ciênc.

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Streptococcus mutans is one of the main factors in formation of cariogenic biofilms. New strategies need to be developed to reduce the formation of cariogenic biofilms. For this purpose, bacterial exopolysaccharides (EPS) could be considered as new agents against biofilms. Therefore, cell-bound (b-EPS) and released exopolysaccharides (r-EPS) were extracted from the strains Apilactobacillus kunkeei K1.10 and Latilactobacillus curvatus Kar.9b isolated from the microbiota of honeybees, and their antibiofilm effects on S. mutans biofilm formation were determined. The highest reduction in biofilm formation was achieved by r-EPS of L. curvatus Kar.9b and A. kunkeei K1.10. Scanning electron micrographs (SEM) showed that r-EPS inhibited biofilm formation by reducing adhesion of S. mutans. To increase the production of r-EPS from A. kunkeei K1.10, the effects of different incubation conditions were also analyzed. The highest EPS production was obtained during 48 h-incubation at 37ºC in a medium containing 1% fructose. r-EPS can be used as a raw material to inhibit cariogenic biofilms. Further studies revealing the detailed structural analysis of r-EPS and the mechanism of action of its antibiofilm effect could be beneficial. Finally, b-EPS and r-EPS from lactic acid bacteria were found to have very different properties in terms of their antibiofilm properties.

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Biocontrol potential of Apilactobacillus kunkeei EIR/BG-1 against infectious diseases in honey bees (Apis mellifera L.)

Cited by 4 publications

References 69 publications

Influence of social lifestyles on host–microbe symbioses in the bees

Influence of social lifestyles on host–microbe symbioses in the bees

Preliminary study of the effects of <i>Apilactobacillus kunkeei</i> EIR/BG-1 and <i>Enterococcus hirae</i> EIR/CM-2 supplementation on<i> in vitro</i> rumen fermentation and microbial population

Exopolysaccharides of lactic acid bacteria isolated from honeybee gut and effects of their antibiofilm activity against Streptococcus mutans

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