Biocontrol of Tomato Bacterial Wilt by Foliar Spray Application of a Novel Strain of Endophytic &lt;i&gt;Bacillus&lt;/i&gt; sp.

Fu, Hui-Zhen; Marian, Malek; Enomoto, Takuo; Hieno, Ayaka; Ina, Hidemasa; Suga, Haruhisa; Shimizu, Mitsuaki

doi:10.1264/jsme2.me20078

Cited by 18 publications

(12 citation statements)

References 66 publications

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“…In the recent years, Bacillus spp. has been widely studied as a potential biological control agent [25] . Bacillus can produce various types of diffusible substances and VOCs with strong inhibitory activity against pathogenic fungi.…”

Section: Discussionmentioning

confidence: 99%

The inhibitory effect of volatile organic compounds produced by Bacillus subtilis CL2 on pathogenic fungi of wolfberry

Ling

Zhao

et al. 2020

J Basic Microbiol

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Bacillus subtilis strain CL2 is antagonistic to wolfberry postharvest pathogenic fungi. In this study, we isolated and screened this strain for in vitro experiments. The result of the two-sealed-base-plates method revealed that volatile organic compounds (VOCs) emitted from the strain CL2 inhibited the hyphal growth of four pathogenic fungi Mucor circinelloides LB1, Fusarium arcuatisporum LB5, Alternaria iridiaustralis LB7, and Colletotrichum fioriniae LB8. After exposure to VOCs for 5 days, the hyphal growth of the pathogen C. fioriniae LB8 was inhibited by 73%. Scanning electron microscopy revealed that the VOCs produced by B. subtilis CL2 caused the mycelium morphology of the pathogenic fungi to deform, twist, fold, and shrink. In the in vivo experiments, we noticed that VOCs could significantly reduce the weight loss rate of wolfberry fruits caused by the pathogenic fungus M. circinelloides LB1 and that the decay incidence rate were caused by the pathogenic fungi F. arcuatisporum LB5, A. iridiaustralis LB7, and C. fioriniae LB8. On the basis of the headspace-gas chromatography-ion mobility spectrometry analysis, seven VOCs produced by strain CL2 were identified. Among them, 2,3-butanedione and 3-methylbutyric acid are the main antifungal active substances. This study investigated the antifungal properties of VOCs produced by the strain CL2 on postharvest pathogenic fungi isolated from wolfberry fruits both in vivo and in vitro, thereby providing the theoretical basis for its future applications. K E Y W O R D S antifungal activity, Bacillus subtilis CL2, HS-GC-IMS, volatile organic compounds (VOCs), wolfberry 1 | INTRODUCTION Wolfberry (Lycium barbarum. L) is a deciduous shrub plant belonging to the family Solanaceae, which is also known as Goji Berry [1]. Being the conventional Chinese medicinal herb for over 2000 years now, wolfberry fruits contain a variety of bioactive substances such as L. barbarum polysaccharide [2], carotenoids, betaine, vitamins, and cerebroside bioactive substances that have now been recognized as the latest "superfruit" in the world [3]. Because

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Section: Discussionmentioning

confidence: 99%

The inhibitory effect of volatile organic compounds produced by Bacillus subtilis CL2 on pathogenic fungi of wolfberry

Ling

Zhao

et al. 2020

J Basic Microbiol

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“…The low infection of B. cinerea in treated grapevine plants may be due to the high amount of P. protegens MP12 detected on the leaves in microbiological analysis conducted after foliar spraying. Fu et al [ 51 ] found about 2.5 log(CFU g −1 ) of Bacillus sp. strains after 14 days after foliar spraying, a very low concentration when compared with the data reported here.…”

Section: Discussionmentioning

confidence: 99%

In Vivo Endophytic, Rhizospheric and Epiphytic Colonization of Vitis vinifera by the Plant-Growth Promoting and Antifungal Strain Pseudomonas protegens MP12

et al. 2021

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An evaluation was conducted of the colonization of Pseudomonas protegens MP12, a plant-growth promoting and antagonistic strain, inoculated in vine plants during a standard process of grapevine nursery propagation. Three in vivo inoculation protocols (endophytic, rhizospheric, and epiphytic) were implemented and monitored by means of both culture-dependent and independent techniques. Endophytic treatment resulted in the colonization of the bacterium inside the vine cuttings, which spread to young leaves during the forcing period. Microscopy analysis performed on transformed dsRed-tagged P. protegens MP12 cells confirmed the bacterium’s ability to penetrate the inner part of the roots. However, endophytic MP12 strain was no longer detected once the plant materials had been placed in the vine nursery field. The bacterium also displayed an ability to colonize the rhizosphere and, when the plants were uprooted at the end of the vegetative season, its persistence was confirmed. Epiphytic inoculation, performed by foliar spraying of cell suspension, was effective in controlling artificially-induced Botrytis cinerea infection in detached leaves. The success of rhizospheric and leaf colonization in vine plants suggests potential for the future exploitation of P. protegens MP12 as biofertilizer and biopesticide. Further investigation is required into the stability of the bacterium’s colonization of vine plants under real-world conditions in vineyards.

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“…Full-length 16S rRNA gene sequences were elucidated as suggested by Fu et al (2020) for a more precise taxonomic classification. The 16S rRNA gene sequences of the strains were submitted to the GenBank database.…”

Section: Methodsmentioning

confidence: 99%

<i>Lysinibacillus xylanilyticus</i> Strain GIC41 as a Potential Plant Biostimulant

et al. 2021

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To identify Lysinibacillus strains with the potential to function as plant biostimulants, we screened 10 previously isolated Lysinibacillus strains from the rhizosphere and soil for their plant growth-promoting (PGP) effects. In vitro tests showed that all strains produced indole-3-acetic acid. In primary screening, the PGP effects of these strains were assessed on spinach seedlings grown on Jiffy-7 pellets; strains GIC31, GIC41, and GIC51 markedly promoted shoot growth. In secondary screening, the PGP efficacies of these three strains were examined using spinach seedlings grown in pots under controlled conditions. Only GIC41 exerted consistent and significant PGP effects; therefore, it was selected for subsequent experiments. The results of 6-week glasshouse experiments revealed that GIC41 markedly increased shoot dry weight by ca. 12–49% over that of the control. The impact of fertilization levels on the PGP efficacy of GIC41 was investigated using pot experiments. The application of a specific level of fertilizer was required for the induction of sufficient PGP effects by this strain. The phylogenetic analysis based on the 16S rDNA sequence identified GIC41 as L. xylanilyticus . Collectively, these results show the potential of strain GIC41 to function as a plant biostimulant.

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Biocontrol of Tomato Bacterial Wilt by Foliar Spray Application of a Novel Strain of Endophytic <i>Bacillus</i> sp.

Cited by 18 publications

References 66 publications

The inhibitory effect of volatile organic compounds produced by Bacillus subtilis CL2 on pathogenic fungi of wolfberry

The inhibitory effect of volatile organic compounds produced by Bacillus subtilis CL2 on pathogenic fungi of wolfberry

In Vivo Endophytic, Rhizospheric and Epiphytic Colonization of Vitis vinifera by the Plant-Growth Promoting and Antifungal Strain Pseudomonas protegens MP12

<i>Lysinibacillus xylanilyticus</i> Strain GIC41 as a Potential Plant Biostimulant

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