Biocontrol of Rhizoctonia solani and Sclerotium rolfsii on tomato by delivering antagonistic bacteria through a drip irrigation system

Curtis, Filippo De; Lima, Giuseppe; Vitullo, D.; Cicco, V. De

doi:10.1016/j.cropro.2010.01.012

Cited by 95 publications

(69 citation statements)

References 39 publications

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“…and Burkholderia cepacia (Gram-), respectively, were used in the following tests in vitro and in vivo. This choice was also supported by previous studies where these two antagonists were tested as biocontrol agents against other fungal pathogens and characterized by their mechanisms of action (i.e., production of antifungal metabolites) (De Curtis et al 2010;Vitullo et al 2012).…”

Section: Characteristics Of Organic Amendmentssupporting

confidence: 52%

Suppressive biomasses and antagonist bacteria for an eco-compatible control of Verticillium dahliae on nursery-grown olive plants

Vitullo

Altieri

Esposito

et al. 2012

Int. J. Environ. Sci. Technol.

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Two organic amendments (OMW-M1 and OMW-M2), based on olive mill wastes (OMWs) subjected to advanced processes of aerobic static storage or composting, were tested for their suppressive activity against Verticillium dahliae, the causal agent of olive Verticillium wilt. OMW-M1 and OMW-M2 drastically inhibited the pathogen growth in vitro and then were further tested in suppressive pot experiments. The amendments, mixed at 15 % (v/v) with a nursery standard plant-growth matrix, were tested alone or in combination with two biocontrol bacteria (Bacillus amyloliquefaciens and Burkholderia cepacia) selected from suppressive soils. All mixtures were artificially contaminated with V. dahliae microsclerotia (MS), the density of which was periodically monitored by either a semi-selective medium or a specific real-time Polymerase Chain Reaction technique. In plant-less pot assays conducted in a growth chamber, OMW-M1 was the most effective amendment, reducing V. dahliae MS density by 100 % after 90 days with respect to the untreated control. In nursery experiments with pot-growing olive plants, OMW-M1, particularly when combined with the biocontrol bacteria, confirmed its strong suppressive activity reducing up to 100 % the density of V. dahliae MS in the rhizosphere behaving even better than a commercial biofungicide (Trichoderma asperellum TV1) used as a control. The best combined treatment also reduced plant mortality and increased root and shoot extension. It is concluded that organic amendments from stabilized olive mill by-products showed positive agronomic and phytosanitary properties on pot-growing olive plants and, particularly when enriched with biocontrol agents, they are potentially suitable for use in sustainable agriculture.

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Section: Characteristics Of Organic Amendmentssupporting

confidence: 52%

Suppressive biomasses and antagonist bacteria for an eco-compatible control of Verticillium dahliae on nursery-grown olive plants

Vitullo

Altieri

Esposito

et al. 2012

Int. J. Environ. Sci. Technol.

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“…Numerous positive traits have been attributed to Pseudomonas strains, ranging from denitrification (Zhang et al, 2011) (e.g. in wastewater treatments) to the degradation of toxic components (Farhadian et al, 2008;Khan et al, 2009) and the promotion of plant growth (Chin-A-Woeng et al, 2003;Daayf et al, 2003;De Curtis et al, 2010;Ghyselinck et al, 2013;Haas & Défago, 2005;Kim & Jeun, 2006;Scherwinski et al, 2008;Yan et al, 2002). However, the genus Pseudomonas also has a negative reputation, as it includes opportunistic human pathogens (Gershman et al, 2008) and plant pathogens (Mansfield et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

An rpoD gene sequence based evaluation of cultured Pseudomonas diversity on different growth media

et al. 2013

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The last decade has shown an increased interest in the utilization of bacteria for applications ranging from bioremediation to wastewater purification and promotion of plant growth. In order to extend the current number of micro-organism mediated applications, a continued quest for new agents is required. This study focused on the genus Pseudomonas, which is known to harbour strains with a very diverse set of interesting properties. The aim was to identify growth media that allow retrieval of a high Pseudomonas diversity, as such increasing the chance of isolating isolates with beneficial properties. Three cultivation media: trypticase soy agar (TSA), potato dextrose agar (PDA) and Pseudomonas isolation agar (PIA) were evaluated for their abilities to grow Pseudomonas strains. TSA and PDA were found to generate the largest Pseudomonas diversity. However, communities obtained with both media overlapped. Communities obtained with PIA, on the other hand, were unique. This indicated that the largest diversity is obtained by sampling from either PDA or TSA and from PIA in parallel. To evaluate biodiversity of the isolated Pseudomonas members on the media, an appropriate biomarker had to be identified. Hence, an introductory investigation of the taxonomic resolution of the 16S rRNA, rpoD, gyrB and rpoB genes was performed. The rpoD gene sequences not only had a high phylogenetic content and the highest taxonomic resolution amongst the genes investigated, it also had a gene phylogeny that related well with that of the 16S rRNA gene.

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“…Burkholderia sp1 had antagonistic ability to Fusarium isolated from sick plants of P. heterophylla, and no pathogenicity to plantlet of P. heterophylla. Various antagonistic microorganisms have different biological control mechanisms, and someone has several mechanisms already known (Mccullagh et al, 1996;De Curtis et al, 2010;Nally et al, 2013). The mechanisms involve antibiotic effect, space and nutrition competition, parasitism and inducing systemic resistance of plant, etc (Inbar et al, 1996;Boopathi and Sankara, 1999;Orwa et al, 2002;Gong et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Screening and Identification of Harmful and Beneficial Microorganisms Associated with Replanting Disease in Rhizosphere Soil of Pseudostellariae heterophylla

Lin¹,

Dai²,

Chen³

et al. 2015

IJAB

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Pseudostellariae heterophylla, an important medicinal plant, has been shown to suffer from serious replanting disease that causes significant declines in both yield and quality. The objective of this study was to isolate the soil-born pathogen and antagonistic microorganism for investigation of the relationship between these microorganism and consecutive monoculture problems of P. heterophylla. In this study, we isolated the bacteria with different morphology from P. heterophylla rhizosphere soil, detected the pathogenicity of these bacteria to P. heterophylla plantlet and antagonistic ability to Fusarium of P. heterophylla biotype, identified their species and analyzed their abundance with qRT-PCR in rhizosphere soil of different planting years. We isolated Enterobacter sp1 and Burkholderia sp1 from P. heterophylla rhizosphere soil, and found Enterobacter sp1 possessed the pathogenicity to P. heterophylla plantlet and Burkholderia sp1 was antagonistic for Fusarium of P. heterophylla biotype. However, Burkholderia sp1 was not the antagonistic microorganism to Enterobacter sp1. The growth of Enterobacter sp1 was stimulated by phenolic acids at a certain concentration nearly closed to that in P. heterophylla rhizosphere soil, which increased as planting years of P. heterophylla increased. On the contrary, the phenolic acids had no promoting effect on the growth of Burkholderia sp1. And with the increase of planting years, the abundance of Burkholderia sp1 decreased and abundance of Enterobacter sp1 increased in the P. heterophylla rhizosphere soil. The results indicated that harmful microorganisms increase and beneficial microorganisms decrease, which were mediated by the accumulated autotoxins in rhizosphere soil of consecutively monocultured P. heterophylla, and hence leads to the imbalance of microbial community structure and the degradation of soil ecological function. So several abatement methods, such as crop rotation, specific microbial fertilizer and organic matter, could be used to improve the structure and functional diversity of microbial community, and overcome the obstacles of continuous cropping.

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Biocontrol of Rhizoctonia solani and Sclerotium rolfsii on tomato by delivering antagonistic bacteria through a drip irrigation system

Cited by 95 publications

References 39 publications

Suppressive biomasses and antagonist bacteria for an eco-compatible control of Verticillium dahliae on nursery-grown olive plants

Suppressive biomasses and antagonist bacteria for an eco-compatible control of Verticillium dahliae on nursery-grown olive plants

An rpoD gene sequence based evaluation of cultured Pseudomonas diversity on different growth media

Screening and Identification of Harmful and Beneficial Microorganisms Associated with Replanting Disease in Rhizosphere Soil of Pseudostellariae heterophylla

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