Biocontrol of Monilinia laxa by Aureobasidium pullulans strains: Insights on competition for nutrients and space

Francesco, Alessandra Di; Ugolini, Luisa; D’Aquino, Salvatore; Pagnotta, Eleonora; Mari, Marta

doi:10.1016/j.ijfoodmicro.2017.02.007

Cited by 74 publications

(54 citation statements)

References 28 publications

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“…Phenotypic microarray plates with different carbon and nitrogen sources can give first insights in the potential for competition between pathogen and antagonist using a nutritional similarity index. To unravel the mode of action of A. pullulans strains against Monilinia laxa in wound protection of peaches, Di Francesco et al (2017) incubated A. pullulans and M. laxa in in vitro assays in peach juice. HPLC analysis of the growth medium revealed that specifically depletion of asparagine as nitrogen source restricts growth of M. laxa .…”

Section: Indirect Interaction With Pathogens: Competitionmentioning

confidence: 99%

Mode of Action of Microbial Biological Control Agents Against Plant Diseases: Relevance Beyond Efficacy

Kolnaar²,

2019

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Microbial biological control agents (MBCAs) are applied to crops for biological control of plant pathogens where they act via a range of modes of action. Some MBCAs interact with plants by inducing resistance or priming plants without any direct interaction with the targeted pathogen. Other MBCAs act via nutrient competition or other mechanisms modulating the growth conditions for the pathogen. Antagonists acting through hyperparasitism and antibiosis are directly interfering with the pathogen. Such interactions are highly regulated cascades of metabolic events, often combining different modes of action. Compounds involved such as signaling compounds, enzymes and other interfering metabolites are produced in situ at low concentrations during interaction. The potential of microorganisms to produce such a compound in vitro does not necessarily correlate with their in situ antagonism. Understanding the mode of action of MBCAs is essential to achieve optimum disease control. Also understanding the mode of action is important to be able to characterize possible risks for humans or the environment and risks for resistance development against the MBCA. Preferences for certain modes of action for an envisaged application of a MBCA also have impact on the screening methods used to select new microbials. Screening of MBCAs in bioassays on plants or plant tissues has the advantage that MBCAs with multiple modes of action and their combinations potentially can be detected whereas simplified assays on nutrient media strongly bias the selection toward in vitro production of antimicrobial metabolites which may not be responsible for in situ antagonism. Risks assessments for MBCAs are relevant if they contain antimicrobial metabolites at effective concentration in the product. However, in most cases antimicrobial metabolites are produced by antagonists directly on the spot where the targeted organism is harmful. Such ubiquitous metabolites involved in natural, complex, highly regulated interactions between microbial cells and/or plants are not relevant for risk assessments. Currently, risks of microbial metabolites involved in antagonistic modes of action are often assessed similar to assessments of single molecule fungicides. The nature of the mode of action of antagonists requires a rethinking of data requirements for the registration of MBCAs.

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Section: Indirect Interaction With Pathogens: Competitionmentioning

confidence: 99%

Mode of Action of Microbial Biological Control Agents Against Plant Diseases: Relevance Beyond Efficacy

Kolnaar²,

2019

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“…Aureobasidium pullulans often occurs under stress conditions that are generally considered to be limiting to microbial growth, such as in refrigerated, frozen, salt-preserved and dried foods (Pitt and Hocking, 1999;Nisiotou et al, 2010), in aviation fuel tanks (Rauch et al, 2006), and on synthetic polymer surfaces (Cappitelli and Sorlini, 2008). The ability of A. pullulans to survive in such unconventional habitats and to outcompete other species has been linked to its great adaptability to novel environments, to its polyextremotolerancethe ability to tolerate a plethora of different stresses (Gostin car et al, 2011) and to its nutritional versatility, which is associated with its wide enzymatic profile (Di Francesco et al, 2017). Its production of antimicrobial compounds and siderophores, and its biofilm formation and other factors are also likely to contribute to its adaptability (Takesako et al, 1993;Wang et al, 2009;Klein and Kupper, 2018).…”

Section: Introductionmentioning

confidence: 99%

Fifty Aureobasidium pullulans genomes reveal a recombining polyextremotolerant generalist

Gostinčar

Turk

Zajc

et al. 2019

Environmental Microbiology

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Summary The black yeast Aureobasidium pullulans is a textbook example of a generalistic and ubiquitous fungus thriving in a wide variety of environments. To investigate whether A. pullulans is a true generalist, or alternatively, whether part of its versatility can be attributed to intraspecific specialization masked by cryptic diversification undetectable by traditional phylogenetic analyses, we sequenced and analysed the genomes of 50 strains of A. pullulans from different habitats and geographic locations. No population structure was observed in the sequenced strains. Decay of linkage disequilibrium over shorter physical distances (<100 bp) than in many sexually reproducing fungi indicates a high level of recombination in the species. A homothallic mating locus was found in all of the sequenced genomes. Aureobasidium pullulans appears to have a homogeneous population genetics structure, which is best explained by good dispersal and high levels of recombination. This means that A. pullulans is a true generalist that can inhabit different habitats without substantial specialization to any of these habitats at the genomic level. Furthermore, in the future, the high level of A. pullulans recombination can be exploited for the identification of genomic loci that are involved in the many biotechnologically useful traits of this black yeast.

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“…One of the first formulated postharvest biocontrol products, now widely used, BioSave TM (JetHarvest Solutions) is based on a saprophytic strain of Pseudomonas syringae active against number of postharvest decaying fungi, but not Monilinia . Many studies since than reported antagonists effective against M. laxa , including Aureobasidium pullulans (Mari et al, 2012; Rungjindamai et al, 2013; Di Francesco et al, 2017); Pantoea agglomerans (Bonaterra et al, 2003); and Bacillus amyloliquefaciens (Rungjindamai et al, 2013) as well as some Pseudomonas spp. (Altindag et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Phyllosphere Fungal Communities of Plum and Antifungal Activity of Indigenous Phenazine-Producing Pseudomonas synxantha Against Monilinia laxa

et al. 2019

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European plum (Prunus domestica L.) is a significant commercial crop in Serbia in terms of total fruit production, and is traditionally processed into slivovitz brandy. The brown rot disease caused by Monilinia laxa drastically reduces plum yield almost every year. Fungal communities associated with leaves and fruits of four local Serbian plum cultivars (Požegača, Ranka, Čačanska Lepotica and Čačanska Rodna) were investigated in two phenological stages during early (May) and late (July) fruit maturation. Alpha diversity indices showed that fungal communities were heterogeneous and Beta diversity indicated that autochthonous fungal communities depended upon seasonal changes and the cultivars themselves. The phylum Ascomycota was the most abundant in all samples, with relative abundance (RA) between 46% in the Požegača cultivar (May) and 89% in the Lepotica cultivar (July). The most abundant genus for all plum cultivars in May was Aureobasidium, with RA from 19.27 to 33.69%, followed by Cryptococcus, with 4.8 to 48.80%. In July, besides Cryptococcus, different genera (Metschnikowia, Fusarium, and Hanseniaspora) were dominant on particular cultivars. Among all cultivable fungi, molecular identification of eleven M. laxa isolates from four plum cultivars was performed simultaneously. Bacterial isolates from the plum phyllosphere were tested for their potential antifungal activity against indigenous M. laxa isolates. The most potent antagonist P4/16_1, which significantly reduced mycelial growth of M. laxa, was identified as Pseudomonas synxantha. Further characterization of P4/16_1 revealed the production of volatile organic compounds and phenazine-1-carboxylic acid (PCA). Crude benzene extract of PCA exhibited 57–63% mycelial growth inhibition of M. laxa. LC/MS analysis of the crude extract confirmed the presence of phenazine derivatives amongst other compounds. Scanning electron microscopy revealed morpho-physiological changes in the hyphae of M. laxa isolates caused by the cell culture and the P. synxantha P4/16_1 crude benzene extract. This is the first report of antagonistic activity of P. synxantha against M. laxa induced by diffusible and volatile antifungal compounds, and it appears to be a promising candidate for further investigation for potential use as a biocontrol agent against brown rot-causing fungi.

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Biocontrol of Monilinia laxa by Aureobasidium pullulans strains: Insights on competition for nutrients and space

Cited by 74 publications

References 28 publications

Mode of Action of Microbial Biological Control Agents Against Plant Diseases: Relevance Beyond Efficacy

Mode of Action of Microbial Biological Control Agents Against Plant Diseases: Relevance Beyond Efficacy

Fifty Aureobasidium pullulans genomes reveal a recombining polyextremotolerant generalist

Phyllosphere Fungal Communities of Plum and Antifungal Activity of Indigenous Phenazine-Producing Pseudomonas synxantha Against Monilinia laxa

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