Biocontrol of Meloidogyne javanica by Rhizobium and plant growth-promoting rhizobacteria on lentil

Abstract

Biocontrol of the root-knot nematode Meloidogyne javanica was studied on lentil using plant growth-promoting rhizobacteria (PGPR) namely Pseudomonas putida, P. alcaligenes, Paenibacillus polymyxa and Bacillus pumilus and root nodule bacterium Rhizobium sp. Pseudomonas putida caused greater inhibitory effect on the hatching and penetration of M. javanica followed by P. alcaligenes, P. polymyxa and B. pumilus. Inoculation of any PGPR species alone or together with Rhizobium increased plant growth both in M. javanica-inoculated and -uninoculated plants. Inoculation of Rhizobum caused greater increase in plant growth than caused by any species of plant growth-promoting rhizobacteria in nematode-inoculated plants. Among PGPR, P. putida caused greater increase in plant growth and higher reduction in galling and nematode multiplication followed by P. alcaligenes, P. polymyxa and B. pumilus. Combined use of Rhizobium with any species of PGPR caused higher reduction in galling and nematode multiplication than their individual inoculation. Use of Rhizobium plus P. putida caused maximum reduction in galling and nematode multiplication followed by Rhizobium plus P. alcaligens. Pseudomonas putida caused greater root colonization and siderophore production followed by P. alcaligenes, P. polymyxa and B. pumilus. Analysis of the protein bands of these four species by SDS-PAGE revealed that P. putida had a different protein band profile compared to the protein profiles of P. alcaligenes, P. polymyxa and B. pumilus. However, the protein profiles of P. acaligenes, P. polymyxa and B. pumilus were similar.

Screening of rhizosphere bacteria from grapevine for their suppressive effect on Xiphinema index Thorne & Allen on in vitro grape plants

“…Plant growth promoting rhizo-and endophytic-bacteria have potential to reduce the damage caused by nematodes. A number of studies have assessed the effect of these bacteria on the endoparasitic nematodes Meloidogyne incognita, Heterodera glycines , M. javanica (Ali et al 2002;Siddiqui et al 2007), Globodera rostochiensis, and Heterodera schachtii (Sikora 1997). However, only a few investigations have been conducted with ectoparasitic nematodes, e.g.…”

A peek into the microbiome of two free-living aquatic nematodes Anticoma & Daptonema

“…Pseudomonas has also been shown to be pathogenic to nematodes [33,34] as well as mutualistic with C. elegans in multi-species environments [35] . Paenibacillus has been shown to be Microbiome of aquatic nematodes 10/14 associated with plants [36,37] and suppress nematodes [38,39] , but has also been shown to have phoretic associations with nematodes [5,40] .…”

Bacterial Determinants and Plant Defense Induction: Their Role as Biocontrol Agents in Sustainable Agriculture

“…The mechanisms by which PGPR control the damage to plants resulting from pathogen invasion include siderophore secretion, physical displacement, and production of antibiotics, enzymes, and a variety of molecules that inhibit phytopathogen growth (Niranjan Raj et al 2006 ). One of the major mechanisms that can control the proliferation of phytopathogens is the production of siderophores with a very much higher affi nity for iron than fungal pathogens (Siddiqui et al 2007 ;Sayyed and Chincholkar 2009 ;Sayyed and Patel 2011 ;Glick 2012 ;Sayyed et al 2013 ;Shaikh et al 2014 ;Shaikh and Sayyed 2015 ). Another effective mechanism is the production of antibiotics, which are deleterious to the metabolism or growth of other pathogens (Doornbos et al 2012 ).…”