Biocontrol of &lt;I&gt;Bactrocera oleae&lt;/I&gt; (Diptera: Tephritidae) With &lt;I&gt;Metarhizium brunneum&lt;/I&gt; and Its Extracts

Yousef-Yousef, Meelad; Tovar, María Denis Lozano; Garrido‐Jurado, Inmaculada; Quesada‐Moraga, Enrique

doi:10.1603/ec12489

Cited by 49 publications

(30 citation statements)

References 33 publications

(37 reference statements)

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“…For example, these isolates also achieved high mortality in prepupae and pupae of the dipteran fruit flies, Ceratitis capitata Wied. and Bactrocera oleae Rossi (Garrido-Jurado et al ., 2011; Yousef et al ., 2013). This is interesting as EPFs often have no or low efficacy against pupae of dipterans because the dipteran puparium is a barrier to penetration and outgrowth of EPFs (Kaaya and Munyinyi, 1995; De la Rosa et al ., 2002; Cossentine et al ., 2010).…”

Section: Discussionmentioning

confidence: 99%

Soil drenching with entomopathogenic fungi for control of the soil-dwelling life stages and adults of the same generation ofSpodoptera littoralis(Boisd.) (Lepidoptera: Noctuidae)

Garrido‐Jurado

Resquín-Romero

Yousef-Yousef

et al. 2019

Bull. Entomol. Res.

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Four Beauveria bassiana and three Metarhizium brunneum isolates were evaluated, as soil drenches, against Spodoptera littoralis prepupae. Treatment efficacy was determined by assessing total mortality during development from prepupae through to pupae and adults; mortality and sub-lethal effects on reproduction were also quantified for adults emerging from surviving prepupae/pupae. All isolates were pathogenic but overall mortality varied between 31.7 and 83.3% (0% for control); average survival time was 7.5–10.5 days (14.0 days for control). From 1.7–15.0% of adults emerging from surviving prepupae/pupae were deformed (0% in control). Contact with fungal suspensions as prepupae/pupae caused a significant reduction in fecundity of emerging adult females (15–58.9%), and a significant reduction in egg viability (6.8–28.4%) compared with controls. Two isolates were selected for virulence evaluation against S. littoralis prepupae. The LC50s were 1.7 × 107 and 1.8 × 107 conidia ml−1 and the median survival times were 7 and 6 days for isolates EAMa 01/58-Su and EAMb 09/01-Su, respectively. Destruxin A was present in pupae developing from prepupae treated with isolates EAMa 01/58-Su (0.010 ± 0.002 µg pupae−1) and EAMb 09/01-Su (0.015 ± 0.003 µg pupae−1). The use of entomopathogenic fungi as soil drenches could be a key component of S. littoralis IPM strategies due to direct reductions in the number of soil-dwelling life stages and, also, the significant reduction in reproductive potential of surviving adults.

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Section: Discussionmentioning

confidence: 99%

Soil drenching with entomopathogenic fungi for control of the soil-dwelling life stages and adults of the same generation ofSpodoptera littoralis(Boisd.) (Lepidoptera: Noctuidae)

Garrido‐Jurado

Resquín-Romero

Yousef-Yousef

et al. 2019

Bull. Entomol. Res.

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“…Bactrocera oleae adults used in the laboratory bioassays were obtained from naturally infested fruit collected from September to December in the Cordoba area. The infested olives were maintained as described by Yousef et al ( 2013 ) to obtain the adults. P. concolor were originally obtained from a population at the Technical University of Madrid (UPM, Madrid, Spain), and then a stock colony was maintained at the Department of Agricultural and Forestry Sciences of the University of Cordoba in a rearing chamber set at 25 ± 2°C, 50–60% RH, and 16:8 h (L:D) following the standard procedure developed by Jacas and Viñuela ( 1994 ).…”

Section: Methodsmentioning

confidence: 99%

Metarhizium brunneum (Ascomycota; Hypocreales) Treatments Targeting Olive Fly in the Soil for Sustainable Crop Production

et al. 2018

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Soil treatments with Metarhizium brunneum EAMa 01/58-Su strain conducted in both Northern and Southern Spain reduced the olive fly (Bactrocera oleae) population density emerging from the soil during spring up to 70% in treated plots compared with controls. A model to determine the influence of rainfall on the conidial wash into different soil types was developed, with most of the conidia retained at the first 5 cm, regardless of soil type, with relative percentages of conidia recovered ranging between 56 and 95%. Furthermore, the possible effect of UV-B exposure time on the pathogenicity of this strain against B. oleae adults coming from surviving preimaginals and carrying conidia from the soil at adult emergence was also evaluated. The UV-B irradiance has no significant effect on M. brunneum EAMa 01/58-Su pathogenicity with B. oleae adult mortalities of 93, 90, 79, and 77% after 0, 2, 4, and 6 of UV-B irradiance exposure, respectively. In a next step for the use of these M. brunneum EAMa 01/58-Sun soil treatments within a B. oleae IPM strategy, its possible effect of on the B. oleae cosmopolitan parasitoid Psyttalia concolor, its compatibility with the herbicide oxyfluorfen 24% commonly used in olive orchards and the possible presence of the fungus in the olive oil resulting from olives previously placed in contact with the fungus were investigated. Only the highest conidial concentration (1 × 108 conidia ml−) caused significant P. concolor adult mortality (22%) with enduing mycosis in 13% of the cadavers. There were no fungal propagules in olive oil samples resulting from olives previously contaminated by EAMa 01/58-Su conidia. Finally, the strain was demonstrated to be compatible with herbicide since the soil application of the fungus reduced the B. oleae population density up to 50% even when it was mixed with the herbicide in the same tank. The fungal inoculum reached basal levels 4 months after treatments (1.6 × 103 conidia g soil−1). These results reveal both the efficacy and environmental and food safety of this B. oleae control method, protecting olive groves and improving olive oil quality without negative effects on the natural enemy P. concolor.

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“…Bacillus thuringiensis strains were found to be somewhat effective but not applicable against tephritid pests due to the insect’s concealed behavior [ 23 ]. The failure of baculovirus and protozoa to cause disease against olive fruit flies [ 24 ] has prompted researchers to focus on entomopathogenic nematodes [ 17 ] and entomopathogenic fungi (EPF). A number of studies have evaluated EPF against Mediterranean fruit fly Ceratitis capitata (Wiedemann) [ 25 , 26 , 27 ], Mexican fruit fly Anastrepha ludens (Loew) [ 28 , 29 , 30 ], European cherry fruit fly, Rhagoletis cerasi (L.) [ 31 ], western cherry fruit fly R. indifferens Curran [ 32 ], B. oleae (Rossi) [ 33 ], and R. pomonella (Walsh) [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Locally Isolated Entomopathogenic Fungi against Multiple Life Stages of Bactrocera zonata and Bactrocera dorsalis (Diptera: Tephritidae): Laboratory and Field Study

et al. 2021

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Fruit flies including Bactrocera zonata and B. dorsalis (Diptera: Tephritidae) are considered major pests of orchard systems in Pakistan. This study evaluated the laboratory virulence, sub-lethal effects, horizontal transmission, greenhouse, and field-cage efficacy of locally isolated entomopathogenic fungi (EPF) against B. zonata and B. dorsalis. In virulence assays against third instars and adults, all 21 EPF isolates (Beauveria bassiana and Metarhizium anisopliae) tested were pathogenic and caused varying levels of mortality to the fruit flies. Based on the initial screening, four isolates (B. bassiana WG-21 and WG-18 and M. anisopliae WG-07 and WG-02) were selected for further study. The isolate WG-18 was the most virulent against larvae and adults of B. zonata and B. dorsalis followed by WG-21, WG-02, and WG-07. In both species, adults were more susceptible than larvae to all isolates, and pupae were the least susceptible. Isolates WG-18 and WG-21 strongly decreased female fecundity and fertility, the highest adult and larval mortality, and longest developmental time of larvae and pupae. Fungal conidia were disseminated passively from infected to healthy adults and induced significant mortality, particularly from infected males to non-infected females. In greenhouse and field-cage experiments, WG-18 and WG-21 were the most effective isolates in reducing adult emergence when applied to larvae and pupae of both fruit fly species. Our results indicate that B. bassiana isolates WG-18 and WG-21 were the most virulent against multiple life stages of B. zonata and B. dorsalis, and also exerted the strongest sub-lethal effects.

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Biocontrol of <I>Bactrocera oleae</I> (Diptera: Tephritidae) With <I>Metarhizium brunneum</I> and Its Extracts

Cited by 49 publications

References 33 publications

Soil drenching with entomopathogenic fungi for control of the soil-dwelling life stages and adults of the same generation ofSpodoptera littoralis(Boisd.) (Lepidoptera: Noctuidae)

Soil drenching with entomopathogenic fungi for control of the soil-dwelling life stages and adults of the same generation ofSpodoptera littoralis(Boisd.) (Lepidoptera: Noctuidae)

Metarhizium brunneum (Ascomycota; Hypocreales) Treatments Targeting Olive Fly in the Soil for Sustainable Crop Production

Evaluation of Locally Isolated Entomopathogenic Fungi against Multiple Life Stages of Bactrocera zonata and Bactrocera dorsalis (Diptera: Tephritidae): Laboratory and Field Study

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