Biocontrol of Listeria monocytogenes by Bacillus coagulans GBI-30, 6086 in a synbiotic white brined cheese: An In Vitro model study

Rugji, Jerina; Dinçoğlu, Ahmet Hulusi

doi:10.1016/j.lwt.2022.113982

Cited by 5 publications

(3 citation statements)

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“…In addition, contamination after low pasteurisation temperature and processing should not be ignored. Apart from this, dairy products produced from raw milk are very likely to be part of this process (Rugji & Dinçoğlu, 2022 ). In this study, as mentioned above, Listeria spp.…”

Section: Discussionmentioning

confidence: 99%

“…Cases continue to occur as a consequence of post‐processing contamination and ingestion of raw milk products, despite the fact that milk pasteurization has decreased the risk (Matto et al., 2018 ). Especially, L. monocytogenes is a danger to dairy products since it can survive and develop at refrigeration temperatures in the presence of moderate salt concentrations and neutral pH activity (Rugji & Dinçoğlu, 2022 ; Rugji et al., 2023 ). L. monocytogenes was detected in 0.51% for all ready to eat milk products ( N = 26.154) in the European Union.…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial resistance and prevalence of Listeria species from raw milk and dairy products in Burdur, Turkey

Erol,

Polat,

Soyuçok

et al. 2024

Veterinary Medicine & Sci

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ObjectivesWorldwide, but especially in emerging nations, concerns about food safety pose a serious obstacle to societal and economic progress. This research aimed to examine the prevalence of Listeria spp. in raw milk and dairy products in Burdur, as well as the presence of genes associated with biofilm formation and antibiotic resistance in the isolates.MethodsA total of 185 samples, including raw milk, curd, cream, butter, yogurt and cheese, were randomly collected in Burdur. The enrichment and isolation methods specified by the United States Department of Agriculture was used to identify Listeria species in milk and dairy product samples. Culture‐positive strains were identified as Listeria genus and as species by PCR. Antibiotic susceptibility of the isolates was evaluated against 14 antibiotics using the disc diffusion technique (EUCAST).ResultsOf them, 2.2% (4/185) were positive for Listeria spp. Listeria species were isolated from cheese and yogurt samples. Two of them were Listeria innocua 1.1% (2/185), one was Listeria ivanovii 0.5% (1/185) and the other was Listeria welshimeri 0.5% (1/185). As a result of multiplex PCR of the biofilm genotypic marker luxS and flaA genes, the flaA gene was detected in three of four isolates, the luxS gene was detected in one isolate, and these two genes were not found in one isolate. Although all isolates were resistant to gentamicin and rifampicin, they also showed multidrug resistance.ConclusionThis study revealed that the diversity of prevalence of Listeria spp. in Burdur requires microbial risk assessment in the milk and dairy products value chain and the need to focus on the problem of multiple antibiotic resistance.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antimicrobial resistance and prevalence of Listeria species from raw milk and dairy products in Burdur, Turkey

Erol,

Polat,

Soyuçok

et al. 2024

Veterinary Medicine & Sci

View full text Add to dashboard Cite

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“…In many studies, it is thought that the addition of natural resources (lactic acid bacteria, probiotics, bacteriocins, bacteriophages, essential oils, plant extracts) to food are effective alternatives in the fight against different types of foodborne diseases. (Rugji & Dincoğlu, 2022 : Siddiqui et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial effects of laurel extract, laurel essential oil, zahter extract, and zahter essential oil on chicken wings contaminated with Salmonella Typhimurium

Yilmaz,

Yalçin,

Polat

2024

Veterinary Medicine & Sci

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BackgroundThis study aimed to evaluate the antimicrobial effects of zahter extract, zahter essential oil, laurel extract, and laurel essential oil on Salmonella Typhimurium inoculated on chicken wings.MethodsA total of 10 groups, including eight study groups and two control groups were formed, consisting of zahter extract and zahter essential oil and laurel extract and laurel essential oil in different proportions. In the study, laurel extract at 6.4% and 12.8% concentrations, laurel essential oil at 0.2% and 0.4% concentrations, zahter extract at 0.2% and 0.4% concentrations, and zahter essential oil at 0.2% and 0.4% concentrations were used.ResultsThe broth microdilution method was used to evaluate the antimicrobial activity of the extract and essential oils on the S. Typhimurium. Minimum inhibitory concentrations of the extracts and essential oils used in the study against S. Typhimurium were determined. The highest inhibitory effect on S. Typhimurium was observed in the 0.4% laurel essential oil group. It was determined that the inhibitory effect increased as the concentration of laurel essential oil increased. In addition, the antimicrobial activity of zahter essential oil is less inhibitory than the laurel extract, laurel essential oil, and zahter extract.ConclusionAccording to the results of this study, it has been revealed that extracts and essential oils obtained from zahter and laurel plants, which have been shown to be natural antimicrobial, can be used in foods as an alternative to chemical additives. To develop research results, the applicability of these extracts and essential oils in different foodstuffs should be examined using different ingredients and concentrations.

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Investigation of the seasonal prevalence, phenotypic, and genotypic characteristics of Listeria monocytogenes in slaughterhouses in Burdur

Erol,

Taşçı

2024

Journal of Applied Microbiology

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Aim This study examined Listeria monocytogenes isolates from two slaughterhouses in Burdur province, southern Turkey, over four seasons for antibiotic resistance, serogroups, virulence genes, in vitro biofilm forming capacity, and genetic relatedness. Methods and results Carcass (540) and environment-equipment surface (180) samples were collected from two slaughterhouses (S1, S2) for 1 year (4 samplings). Of the 89 (12.4%) positive isolates, 48 (53.9%) were from animal carcasses and 41 (46.1%) from the environment-equipment surfaces. Autumn was the peak season for Listeria monocytogenes compared to summer and spring (P < 0.05). In addition, the most common serotype between seasons was 1/2c. Except for plcA and luxS genes, all isolates (100%) harbored inlA, inlC, inlJ, hlyA, actA, iap, flaA genes. L. monocytogenes isolates were identified as belonging to IIc (1/2c-3c; 68.5%), IVb (4b-4d-4e; 29.2%), and IIa (1/2a-3a; 2.2%) in the screening using multiplex PCR-based serogrouping test. A total of 65 pulsotypes and 13 clusters with at least 80% homology were determined by using Pulsed Field Gel Electrophoresis on samples that had been digested with ApaI. Thirty-four (38.2%) of the isolates were not resistant to any of the 14 antibiotics tested. The antibiotic to which the isolates showed the most resistance was rifampicin (44.9%). Serotype 1/2c was the most resistant serotype to antibiotics. Despite having biofilm-associated genes (inlA, inlB, actA, flaA, luxS), a minority (11%) of isolates formed weak biofilm. Conclusion This study revealed seasonal changes prevalence of Listeria monocytogenes, particularly higher in autumn, posing a greater risk of meat contamination. Notably, Serotype 1/2c showed significant prevalence and antibiotic resistance. Indistinguishable isolates indicated cross-contamination, underscoring the importance of prioritized training for slaughterhouse personnel in sanitation and hygiene protocols.

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Biocontrol of Listeria monocytogenes by Bacillus coagulans GBI-30, 6086 in a synbiotic white brined cheese: An In Vitro model study

Cited by 5 publications

References 49 publications

Antimicrobial resistance and prevalence of Listeria species from raw milk and dairy products in Burdur, Turkey

Antimicrobial resistance and prevalence of Listeria species from raw milk and dairy products in Burdur, Turkey

Antimicrobial effects of laurel extract, laurel essential oil, zahter extract, and zahter essential oil on chicken wings contaminated with Salmonella Typhimurium

Investigation of the seasonal prevalence, phenotypic, and genotypic characteristics of Listeria monocytogenes in slaughterhouses in Burdur

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