Biocontrol of Citrus Canker Disease Caused by Xanthomonas citri subsp. citri Using an Endophytic Bacillus thuringiensis

Islam, Md. Nurul; Ali, Md. Sarafat; Choi, Su-Lim; Hyun, Jae-Wook; Baek, Kwang‐Hyun

doi:10.5423/ppj.oa.03.2019.0060

Cited by 46 publications

(45 citation statements)

References 54 publications

(53 reference statements)

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“…For many years, the most common approach to controlling citrus canker has been to eradicate symptomatic citrus trees to prevent the spread of the pathogens. Spraying of chemical bactericides such as copper and antibiotics is widely applied to prevent and treat Xcc infections [ 5 , 6 , 7 ]. However, the excessive and frequent use of these chemical pesticides has caused environmental pollution, pesticide residues and drug resistance [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, there is an urgent need to search for new alternatives to develop safe, nontoxic and environmentally friendly bactericides to control this disease. In recent years, biological control—i.e., the use of biocontrol agents or their biologically active metabolites—has attracted much attention [ 5 , 9 , 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

“…Microorganisms that are used as biocontrol agents have the characteristics of high specificity against target pathogens and low mass production cost [ 13 ]. Bacillus is currently the most studied antagonistic microorganism, and there are numerous reports about its inhibitory effect on a variety of phytopathogens [ 5 , 10 , 14 , 15 , 16 , 17 , 18 ], induction of plant system resistance and improvement of plant response to different stresses [ 19 , 20 ]. The feature contributing to its success is its capacity to produce many biologically active metabolites, especially the nonribosomal synthetic cyclic lipopeptides surfactin, iturin and fengycin.…”

Section: Introductionmentioning

confidence: 99%

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Isolation of the Novel Strain Bacillus amyloliquefaciens F9 and Identification of Lipopeptide Extract Components Responsible for Activity against Xanthomonas citri subsp. citri

Wang

Liang

Shao

et al. 2022

Plants

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Citrus canker, caused by Xanthomonas citri subsp. citri (Xcc), is a quarantine disease that seriously affects citrus production worldwide. The use of microorganisms and their products for biological control has been proven to be effective in controlling Xanthomonas disease. In this study, a novel Xcc antagonistic strain was isolated and identified as Bacillus amyloliquefaciens F9 by morphological and molecular analysis. The lipopeptide extract of B. amyloliquefaciens F9 (F9LE) effectively inhibited the growth of Xcc in an agar diffusion assay and restrained the occurrence of canker lesions in a pathogenicity test under greenhouse conditions. Consistent with these findings, F9LE treatment significantly inhibited the production of extracellular enzymes in Xcc cells and induced cell wall damage, with leakage of bacterial contents revealed by scanning electron microscopy and transmission electron microscopy analyses. In addition, F9LE also showed strong antagonistic activity against a wide spectrum of plant pathogenic bacteria and fungi. Furthermore, using electrospray ionization mass spectrometry analysis, the main antimicrobial compounds of strain F9 were identified as three kinds of lipopeptides, including homologues of surfactin, fengycin, and iturin. Taken together, our results show that B. amyloliquefaciens F9 and its lipopeptide components have the potential to be used as biocontrol agents against Xcc, and other plant pathogenic bacteria and fungi.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Isolation of the Novel Strain Bacillus amyloliquefaciens F9 and Identification of Lipopeptide Extract Components Responsible for Activity against Xanthomonas citri subsp. citri

Wang

Liang

Shao

et al. 2022

Plants

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“…Citri, and X. citri pv. punicae with bacterial wilt of tomato, citrus canker, and bacterial leaf blight of pomegranate, respectively [33][34][35].…”

Section: Discussionmentioning

confidence: 99%

Colloidal Silver Hydrogen Peroxide: New Generation Molecule for Management of Phytopathogens

et al. 2021

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Plant pathogenic fungi and bacteria are a significant threat to global commercial crop production resulting in increased cost of production, reduced crop establishment and productivity. An effort was made to study the antimicrobial activity of silver hydrogen peroxide (SHP) against selected plant pathogenic fungi and bacteria under in vitro conditions. Higher antibacterial activity of SHP was observed against Xanthomonas axonopodis pv. citri (Xac; 39.67 mm), Xanthomonas citri pv. punicae (Xap; 39.00 mm), and Ralstonia solanacearum (Rs; 36.67 mm) at 500 ppm concentration. SHP was superior to streptocycline (500 ppm) against Xac (25.33 mm) and Xcp (22.67 mm) at 100 ppm. The soil-borne fungi viz., Pythium aphanidermatum and Fusarium solani failed to initiate mycelium growth on PDA at the concentration of 5000 ppm and above. The average size of SHP particles was 462 nm in diameter, and 73.40% of particles had the size of 378 nm, which reflects the particles present in SHP solution in the form of colloids. The effective doses (100–5000 ppm) did not show any phytotoxicity symptoms in plants, while leaf necrosis was noticed at 10,000 ppm after four days of application. SHP (≤5000 ppm) can be used to effectively manage both fungal and bacterial plant pathogens by a single application. Further field studies need to be conducted for validation and commercial use of SHP.

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“…Asiatic Citrus Canker, caused by the Gram-negative bacterium Xanthomonas citri subsp. citri, is an example of an infectious disease that affects all the commercially important citrus varieties (Gottwald et al 2002;Islam et al 2019). It is considered one of the most severe and widespread diseases of citrus known.…”

Section: Introductionmentioning

confidence: 99%

Hexanoic acid: a new potential substitute for copper‐based agrochemicals against citrus canker

et al. 2021

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Aims: The aim of the study is to evaluate hexanoic acid (HA) as an alternative to manage citrus canker. Methods and Results: The minimal growth inhibitory concentration of HA against Xanthomonas citri subsp. citri was determined at 2Á15 mmol l −1 using a respiratory activity assay. Growth curves at different pH values showed that growth inhibition was not due to media acidification induced by HA. The germination rate and root elongation of Lactuca sativa seeds exposed to different concentrations of HA (varying from 0Á86 to 5Á16 mmol l −1 ) were assessed to screen for phytotoxicity. The acid exhibited low phytotoxicity for L. sativa at 1Á29 and 2Á58 mmol l −1 . To evaluate the ability of HA to protect citrus against X. citri infection, leaves of Citrus sinensis were sprayed with the acid and subsequently challenged with X. citri. HA at 3Á44 mmol l −1 was able to protect citrus against infection, showing a reduction of three orders of magnitude in the number of citrus canker lesions per cm 2 when compared to the untreated negative control. Conclusion: HA is a potential alternative to copper for citrus canker management. Significance and Impact of the Study: HA inhibits X. citri growth, exhibits low phytotoxicity and is an alternative to copper for the protection of citrus plants against bacterial infection.

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Biocontrol of Citrus Canker Disease Caused by Xanthomonas citri subsp. citri Using an Endophytic Bacillus thuringiensis

Cited by 46 publications

References 54 publications

Isolation of the Novel Strain Bacillus amyloliquefaciens F9 and Identification of Lipopeptide Extract Components Responsible for Activity against Xanthomonas citri subsp. citri

Isolation of the Novel Strain Bacillus amyloliquefaciens F9 and Identification of Lipopeptide Extract Components Responsible for Activity against Xanthomonas citri subsp. citri

Colloidal Silver Hydrogen Peroxide: New Generation Molecule for Management of Phytopathogens

Hexanoic acid: a new potential substitute for copper‐based agrochemicals against citrus canker

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