Biocontrol of chocolate spot disease (<i>Botrytis cinerea</i>) in faba bean using endophytic actinomycetes<i>Streptomyces</i>: a field study to compare application techniques

El-Shatoury, Sahar A.; Ameen, Fuad; Moussa, Hanan; Wahid, Omar A. Abdul; Dewedar, Ahmed; AlNadhari, Saleh

doi:10.7717/peerj.8582

Cited by 16 publications

(5 citation statements)

References 38 publications

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“… Antifungal activity (% growth inhibition * ) of actinomycetes in vivo isolated from different environments [ 1 , 4 , 10 , 21 , 25 , 29 , 59 , 60 , 63 , 69 , 70 , 71 , 73 , 74 , 77 , 78 , 80 , 83 , 85 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 ]. …”

Section: Figurementioning

confidence: 99%

Actinomycete Potential as Biocontrol Agent of Phytopathogenic Fungi: Mechanisms, Source, and Applications

Torres-Rodríguez¹,

Pérez

Quiñones-Aguilar³

et al. 2022

Plants

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Synthetic fungicides have been the main control of phytopathogenic fungi. However, they cause harm to humans, animals, and the environment, as well as generating resistance in phytopathogenic fungi. In the last few decades, the use of microorganisms as biocontrol agents of phytopathogenic fungi has been an alternative to synthetic fungicide application. Actinomycetes isolated from terrestrial, marine, wetland, saline, and endophyte environments have been used for phytopathogenic fungus biocontrol. At present, there is a need for searching new secondary compounds and metabolites of different isolation sources of actinomycetes; however, little information is available on those isolated from other environments as biocontrol agents in agriculture. Therefore, the objective of this review is to compare the antifungal activity and the main mechanisms of action in actinomycetes isolated from different environments and to describe recent achievements of their application in agriculture. Although actinomycetes have potential as biocontrol agents of phytopathogenic fungi, few studies of actinomycetes are available of those from marine, saline, and wetland environments, which have equal or greater potential as biocontrol agents than isolates of actinomycetes from terrestrial environments.

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Section: Figurementioning

confidence: 99%

Actinomycete Potential as Biocontrol Agent of Phytopathogenic Fungi: Mechanisms, Source, and Applications

Torres-Rodríguez¹,

Pérez

Quiñones-Aguilar³

et al. 2022

Plants

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“…Asaturova et al (2022), evaluating seed and plant treatment with Bacillus strains against P. tritici-repentis, also observed that the treatment of seeds and plants with strain BZR336 was 10% higher than when treating the plants alone. Other authors also observed that cell-free supernatant of Streptomyces isolates reduced leaf spots of plants infected with Botrytis cinerea and Alternaria brassicicola, and contributed to increased dry weight of plants (El-Shatoury et al 2020;Sharma and Manhas 2022).…”

Section: Discussionmentioning

confidence: 87%

Antifungal activity of Streptomyces spp. against Pyrenophora tritici-repentis the causal agent of tan spot in wheat

2023

Journal of Plant Protection Research

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Tan spot, caused by Pyrenophora tritici-repentis (Ptr), is a worrisome destructive foliar disease of wheat-growing areas around the world. Streptomyces spp. have been investigated as biocontrol agents because they beneficially interact with host plants and produce important bioactive substances that can act in the suppression of diseases in plants. In the present study, antifungal activity and plant growth-promoting of Streptomyces spp. strains 6(4), R18(6), and their consortium, were evaluated through in vitro and greenhouse assays. The Ultra High-Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry (UHPLC-QTOF MS) technique was used to analyze the crude extract of each strain. The results of the in vitro tests showed that the 6(4) metabolites caused several abnormalities in the conidial germination of Ptr. This strain also produced indole acetic acid (IAA) and siderophores. Strain R18(6) did not alter conidial germination of Ptr, and produced IAA and phosphate solubilizers. In the greenhouse, the treatment 'seed inoculation plus foliar spray' with streptomycetes propagules and metabolites contributed to biomass gain, with no statistical difference between the strains (p < 0.05). Treatments with 6(4) 'seed inoculation' , 'seed inoculation plus foliar spray' , and consortium 'seed inoculation' showed the lowest percentage of injured area compared to other treatments (p < 0.05). UHPLC-QTOF MS data showed that erucamide is present in the culture of 6(4), but not in the culture of R18(6). Therefore, this substance is one of those involved in Ptr hyphal abnormalities, and R18(6) use indirect mechanisms of action to control Ptr. We concluded that these Streptomyces spp. and their metabolites have a promising potential for biological control of Ptr to protect wheat plants from tan spot damage.

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“…Once associated with plant rhizosphere and tissues, actinomycetes counteract microbial phytopathogens by promoting plant growth and development through the solubilization of phosphates, indoleacetic acid, hydrogen cyanide, and ammonia production [59]. Actinomycetes also induce systemic resistance [60], promoting a faster and stronger response of plants to pathogenic attacks [61].…”

Section: Discussionmentioning

confidence: 99%

Biocontrol of Soil-Borne Pathogens of Solanum lycopersicum L. and Daucus carota L. by Plant Growth-Promoting Actinomycetes: In Vitro and In Planta Antagonistic Activity

et al. 2021

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Biotic stress caused by pathogenic microorganisms leads to damage in crops. Tomato and carrot are among the most important vegetables cultivated worldwide. These plants are attacked by several pathogens, affecting their growth and productivity. Fourteen plant growth-promoting actinomycetes (PGPA) were screened for their in vitro biocontrol activity against Solanum lycopersicum and Daucus carota microbial phytopathogens. Their antifungal activity was evaluated against Fusarium oxysporum f. sp. radicis-lycopersici (FORL) and Rhizoctonia solani (RHS). Antibacterial activity was evaluated against Pseudomonas syringae, Pseudomonas corrugata, Pseudomonas syringae pv. actinidiae, and Pectobacterium carotovorum subsp. carotovorum. Strains that showed good in vitro results were further investigated in vitro (cell-free supernatants activity, scanning electron microscope observations of fungal inhibition). The consortium of the most active PGPA was then utilized as biocontrol agents in planta experiments on S. lycopersicum and D. carota. The Streptomyces albidoflavus H12 and Nocardiopsis aegyptica H14 strains showed the best in vitro biocontrol activities. The diffusible and volatile compounds and cell-free supernatants of these strains showed both antifungal (in vitro inhibition up to 85%, hyphal desegregation and fungicidal properties) and antibacterial activity (in vitro inhibition >25 mm and bactericidal properties). Their consortium was also able to counteract the infection symptoms of microbial phytopathogens during in planta experiments, improving plant status. The results obtained highlight the efficacy of the selected actinomycetes strains as biocontrol agents of S. lycopersicum and D. carota.

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Biocontrol of chocolate spot disease (Botrytis cinerea) in faba bean using endophytic actinomycetesStreptomyces: a field study to compare application techniques

Cited by 16 publications

References 38 publications

Actinomycete Potential as Biocontrol Agent of Phytopathogenic Fungi: Mechanisms, Source, and Applications

Actinomycete Potential as Biocontrol Agent of Phytopathogenic Fungi: Mechanisms, Source, and Applications

Antifungal activity of Streptomyces spp. against Pyrenophora tritici-repentis the causal agent of tan spot in wheat

Biocontrol of Soil-Borne Pathogens of Solanum lycopersicum L. and Daucus carota L. by Plant Growth-Promoting Actinomycetes: In Vitro and In Planta Antagonistic Activity

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