Biocontrol Efficacy of Endophytic Bacteria Flavobacterium hercynim EPB-C313 for Control of Chinese Cabbage Clubroot

Abstract

Clubroot of Chinese cabbage by Plasmodiophora brassicae, was found to be high virulent to the Chinese cabbage, turnips and cabbage. It this study, the endophytic bacteria Flavobacterium hercynium EPB-C313, which was isolated from tissues of Chinese cabbage, was investigated the antimicrobial activity on the inactivation of resting spores and its control effect on clubroot disease by bioassay. The bacterial cells, culture solutions, and culture filtrates of F. hercynium EPB-C313 inactivate the resting spores of P. brassicae with 90.4, 36.8, and 26.0%, respectively. The clubroot was inhibited with 100% by dipping the seedlings of Chinese cabbage in culture solutions of F. hercynium EPB-C313 before planting, however the chemmical 'fluazinam' was 91.7% in pot tests. Complex treatment were highly enhanced control efficacy with 63.7% at field of 50% diseased plants by soil incorporation with the pellet contains organic matter and F. hercynium EPB-C313, seedling drench of culture solution of F. hercynium EPB-C313 and soil drench with 10 days after planting. These results imply that the F. hercynium EPB-C313 is a very useful biological control agent of clubroot disease of Chinese cabbage.

Bacterial composition in brassica‐cultivated soils with low and high severity of clubroot

“…Some species of the genera Flavisolibacter, Massilia and Pseudomonas have been related to the suppression of tipping disease caused by Rhizoctonia solani in organic lettuce crops (Bonanomi et al., 2018). The endophytic bacterium Flavobacterium hercynium , isolated from chinese cabbage, showed antimicrobial activity inactivating the spores of P. brassicae and confirmed the efficacy in the biological control of clubroot (Hahm et al., 2012). Bacillus subtilis is cited as an antagonist to P. brassicae (Einhorn, Bochow, Huber, & Krebs, 1991) and B. cereus , B. megaterium , e. B. toyonensis, isolated from tomato plants in these same areas, are cited as antagonists to Fusarium oxysporum f sp.…”

Endosphere microbiome comparison between symptomatic and asymptomatic roots of Brassica napus infected with Plasmodiophora brassicae

“…brassicae [ 44 , 46 , 47 ]; Streptomyces spp. [ 48 , 49 ] and Streptomyces griseoruber A316 [ 50 ] from the family Streptomycetaceae have been reported to reduce the severity of clubroot disease; Flavobacteriumhercynim EPB-C313, an endophytic bacteria of the family Flavobacteriaceae, has been used to control Chinese cabbage clubroot [ 51 ]. In this study, we identified 1, 1, and 7 OTUs in the asymptomatic samples belonging to Bacillaceae, Streptomycetaceae, and Flavobacterium families, respectively.…”

Control of Streptomyces alfalfae XY25T Over Clubroot Disease and Its Effect on Rhizosphere Microbial Community in Chinese Cabbage Field Trials

“…In this study, the largest bacterial group at the genus level was Flavobacterium, whose abundance in the treatment group was about 2% higher than that in the control group, but there was no significant difference between these two groups. Flavobacterium hercynium EPB-C313 has been found to have a significant inhibitory effect on P. brassicae (Hahm et al, 2012). A previous study has shown that Bacillus subtilis XF-1 can promote the growth of Flavobacterium and that Flavobacterium has a potential role in the prevention and treatment of clubroot disease (Liu et al, 2018).…”