Bioconjugated quantum dots as fluorescent probes for bioanalytical applications

Frasco, Manuela F.; Chaniotakis, Nikos A.

doi:10.1007/s00216-009-3033-0

Cited by 125 publications

(74 citation statements)

References 91 publications

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“…Quantum dot nanoparticles have received attention due to their fluorescent characteristics and potential use in medical applications [34,44] . Because the skin is the main target tissue for nanoparticle exposure, assessment of the skin penetration of quantum dots has attracted a great deal of attention.…”

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo percutaneous absorption of seleno-L-methionine, an antioxidant agent, and other selenium species

Lin

Fang²,

Al‐Suwayeh

et al. 2011

Acta Pharmacol Sin

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Aim: To investigate the in vitro and in vivo percutaneous absorption of seleno-L-methionine (Se-L-M), an ultraviolet (UV)-protecting agent, from aqueous solutions. Methods: Aqueous solutions of Se-L-M were prepared in pH 4, 8, and 10.8 buffers. The pH 8 buffer contained 30% glycerol, propylene glycol (PG) and polyethylene glycol (PEG) 400. The in vitro skin permeation of Se-L-M via porcine skin and nude mouse skin was measured and compared using Franz diffusion cells. The in vivo skin tolerance study was performed, which examined transepidermal water loss (TEWL), skin pH and erythema. Results: In the excised porcine skin, the flux was 0.1, 11.4 and 8.2 μg· cm -2 ·h -1 for the pH 4, 8, and 10.8 buffers, respectively. A linear correlation between the flux and skin deposition was determined. According to permeation across skin with different treatments (stripping, delipidation, and ethanol treatments), it was determined that the intracellular route comprised the predominant pathway for Se-L-M permeation from pH 8 buffer. Aqueous solutions of seleno-DL-methionine (Se-DL-M), selenium sulfide and selenium-containing quantum dot nanoparticles were also used as donor systems. The DL form showed a lower flux (7.0 vs 11.4 μg· cm -2 ·h -1 ) and skin uptake (23.4 vs 47.3 μg/g) as compared to the L form, indicating stereoselective permeation of this compound. There was no or only negligible permeation of selenium sulfide and quantum dots into and across the skin. With in vivo topical application for 4 and 8 h, the skin deposition of Se-L-M was about 7 μg/g, and values were comparable to each other. The topical application of Se-L-M for up to 5 d did not caused apparent skin irritation. However, slight inflammation of the dermis was noted according to the histopathological examination. Conclusion: Se-L-M was readily absorbed by the skin in both the in vitro and in vivo experiments. The established profiles of Se-L-M skin absorption will be helpful in developing topical products of this compound.Keywords: seleno-L-methionine; selenium; percutaneous absorption; topical delivery; skin [5] . In humans, a low selenium status is associated with up to a 4-fold increased risk of developing skin cancer [6] . Topical selenium compounds also increase the minimum dose of UV radiation required to cause skin reddening and protect against skin damage caused by UVB [7,8] . Seleno-L-methionine (Se-L-M), the major component of dietary selenium, represents an organic form of selenium which may provide enhanced protection of the skin [9] . Se-L-M also decreases the oxidative stress of neurodegenerative diseases, rheumatoid arthritis, and HIV/AIDS [10,11] . Most ingested selenium, whether in organic or inorganic form, is converted by liver metabolism [3] . In addition, selenium toxicity can produce gastrointestinal problems [12] . Delivery of pharmacologically active compounds via the skin is an attractive alternative to oral dosing for numerous reasons including stable plasma concentrations, bypass of firstpass effects, and reduction of some si...

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Section: Discussionmentioning

confidence: 99%

In vitro and in vivo percutaneous absorption of seleno-L-methionine, an antioxidant agent, and other selenium species

Lin

Fang²,

Al‐Suwayeh

et al. 2011

Acta Pharmacol Sin

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“…Such nanoparticles have been proposed for applications of laser source, bioimaging, and solar energy OPEN ACCESS conversion, with their special optical properties of high PL yields and tunability of absorption and PL wavelengths [1][2][3][4][5]. A large number of theoretical and experimental studies have been devoted to examine the optical properties of nanometer-sized materials [6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

“…Cadmium selenide (CdSe) nanoparticle is one of the semiconductor nanoparticles that have been widely studied and proposed for optoelectronic and biomedical applications [1][2][3][4][5][23][24][25]. In the present study, we measured electrophotoluminescence spectra and field-induced change in the PL decay profile of CdSe nanoparticles in a poly(methyl methacrylate) (PMMA) film, to examine the Stark shift of the PL spectra and the electric field effects on photoexcitation dynamics.…”

Section: Introductionmentioning

confidence: 99%

Electric Field Effects on Photoluminescence of CdSe Nanoparticles in a PMMA Film

2014

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External electric field effects on spectra and decay of photoluminescence (PL) as well as on absorption spectra were measured for CdSe nanoparticles in a poly(methyl methacrylate) (PMMA) film. Electrophotoluminescence (E-PL) spectra as well as electroabsorption spectra show a remarkable Stark shift which depends on the particle size, indicating a large electric dipole moment in the first exciton state. The E-PL spectra also show that PL of CdSe is quenched by application of electric fields, and the magnitude of the field-induced quenching becomes larger with increasing size. The PL decay profiles observed in the absence and presence of electric field show that the field-induced quenching of PL mainly originates from the field-induced decrease in population of the emitting state prepared through the relaxation from the photoexcited state.

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“…Currently, both types of QDs have been derivatized to proteins, oligonucleotides, and other biomolecules using straightforward bioconjugation methods ( Fig. 1A) and have been used routinely in cellular targeting, sensing, and imaging (Carion et al 2007;Frasco and Chaniotakis 2010;Liu et al 2008;Susumu et al 2009). QD-protein conjugates can be used as probes toward specific biological mechanisms in immunoassays and live-cell imaging as well as in a variety of other fluorescence-based detection assays, including SPT and FRET.…”

Section: Quantum Dots In Bioimagingmentioning

confidence: 99%

Quantum Dots in Cell Biology

Barroso

2011

J Histochem Cytochem.

137

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SummaryQuantum dots are semiconductor nanocrystals that have broad excitation spectra, narrow emission spectra, tunable emission peaks, long fluorescence lifetimes, negligible photobleaching, and ability to be conjugated to proteins, making them excellent probes for bioimaging applications. Here the author reviews the advantages and disadvantages of using quantum dots in bioimaging applications, such as single-particle tracking and fluorescence resonance energy transfer, to study receptor-mediated transport. (J Histochem Cytochem 59:237-251, 2011)

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Bioconjugated quantum dots as fluorescent probes for bioanalytical applications

Cited by 125 publications

References 91 publications

In vitro and in vivo percutaneous absorption of seleno-L-methionine, an antioxidant agent, and other selenium species

In vitro and in vivo percutaneous absorption of seleno-L-methionine, an antioxidant agent, and other selenium species

Electric Field Effects on Photoluminescence of CdSe Nanoparticles in a PMMA Film

Quantum Dots in Cell Biology

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