Bioconjugated nanoparticle detection of respiratory syncytial virus infection

Abstract

Abstract:The integration of nanotechnology with biology has produced major advances in molecular diagnostics, therapeutics, and bioengineering. Recent advances have led to the development of functionalized nanoparticles (NPs) that are covalently linked to biological molecules such as antibodies, peptides, proteins, and nucleic acids. These functionalized NPs allow for development of novel diagnostic tools and methods, particularly for pathogens, as rapid and sensitive diagnostics are essential for defi ning the emergence of infection, determining the period that preventive measures should be applied, for evaluating drug and vaccine effi cacy, and for controlling epidemics. In this study, we show that functionalized NPs conjugated to monoclonal antibodies can be used to rapidly and specifi cally detect respiratory syncytial virus in vitro and in vivo. These results suggest that functionalized NPs can provide direct, rapid, and sensitive detection of viruses and thereby bridge the gap between current cumbersome virus detection assays and the burgeoning need for more rapid and sensitive detection of viral agents.

Nanomaterials for Management of Lung Disorders and Drug Delivery

“…For example, Akerman et al [86] demonstrated that QDs coated with a peptide that binds to membrane on pulmonary endothelial cells were detected in the lung but not in brain or kidney after 5 minutes of intravenous administration in BALB/c mice. Furthermore, in a study using QDs conjugated to anti-Respiratory syncytial virus (RSV) protein monoclonal antibody, rapid and specific detection of RSV infection was demonstrated in vitro and in the lungs of BALB/c mice in vivo [87]. QDs have also been used to study tumor cell extravasation into lung tissue in C57BL/6 mice [88], highlighting the utility of these NPs in the study of tumor metastasis.…”

Fluorescent nanocrystal quantum dots as medical diagnostic tools

“…Recently, Tripp et al [51] have shown that functionalized QDs conjugated to monoclonal antibodies can be used to detect rapidly and specifically respiratory syncytial virus in vitro and in vivo . These results suggest that functionalized QDs can provide direct, rapid and sensitive detection of viruses and thereby bridge the gap between current cumbersome virus detection assays and the burgeoning need for more rapid and sensitive detection of viral agents.…”

Short communication: carboxylate functionalized superparamagnetic iron oxide nanoparticles (SPION) for the reduction of S. aureus growth post biofilm formation

“…[3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22] In particular, due to their magnetic properties and location in the body which can be controlled externally through an applied electric field, superparamagnetic iron oxide nanoparticles (SPION) have a strong potential to disrupt biofilms produced by MRSA. [4][5][6]19 These iron oxide nanoparticles (which can be in the form of maghemite, γ-Fe 2 O 3, or magnetite, Fe 3 O 4 ) are materials that can control drug delivery via an external magnetic source, the ability of which can be further improved by functionalizing their surface with molecules or chemistries to better penetrate biofilms and bacteria.…”