Bioconjugated gold nanoparticles as a molecular based contrast agent: implications for imaging of deep tumors using optoacoustic tomography

Copland, John A.; Eghtedari, Mohammad; Popov, Vsevolod L.; Kotov, Nicholas A.; Mamedova, Natasha; Motamedi, Massoud; Oraevsky, Alexander A.

doi:10.1016/j.mibio.2004.06.002

Cited by 293 publications

(205 citation statements)

References 23 publications

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“…Potential imaging mechanisms include optical coherence tomography (OCT), reflectance confocal microscopy (RCM) and optoacoustic tomography [37]. While these 'optical biopsies' achieve a resolution sufficient for the early diagnosis of disease based on cellular anatomy, their contrast is not ideally suited for imaging specific molecular changes.…”

Section: Biomedical Imaging Contrastmentioning

confidence: 99%

“…These nanoparticles have been conjugated to specifically target cancer cells in vitro, where their specificity has been confirmed by dark-field optical microscopy ( Figure 3). Nanoshells and nanocages have also been demonstrated to provide enhanced contrast OCT in vitro [19,41] and gold nanospheres and nanorods have enhanced optoacoustic tomography [37].…”

Section: Biomedical Imaging Contrastmentioning

confidence: 99%

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Biomedical Applications of Plasmon Resonant Metal Nanoparticles

2006

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The strong optical absorption and scattering of noble metal nanoparticles is due to an effect called localized surface plasmon resonance, which enables the development of novel biomedical applications. The resonant extinction, which can be tuned to the near-infrared, allows the nanoparticles to act as molecular contrast agents in a spectral region where tissue is relatively transparent. The localized heating due to resonant absorption, also tunable into the near-infared, enables new thermal ablation therapies and drug delivery mechanisms. The sensitivity of these resonances to their environment leads to simple affinity sensors for the detection of low-level molecular analytes. Coupled with their general lack of toxicity, these applications suggest that noble metal nanoparticles are a highly promising class of nanomaterials for new biomedical applications.

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Section: Biomedical Imaging Contrastmentioning

confidence: 99%

Section: Biomedical Imaging Contrastmentioning

confidence: 99%

Biomedical Applications of Plasmon Resonant Metal Nanoparticles

2006

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“…In the past, we have demonstrated the use of spherical gold nanoparticles for the molecular-specific detection of cancer cells in vitro using optoacoustic methods. 9 Considering the peak absorption of spherical gold nanoparticles that are located at the visible region of the spectrum and the fact that visible light cannot penetrate deep within tissue, one would conclude that spherical gold nanoparticles are not suitable for in vivo optoacoustic detection. Unlike visible light, NIR light penetrates deep within tissue.…”

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confidence: 99%

High Sensitivity of In Vivo Detection of Gold Nanorods Using a Laser Optoacoustic Imaging System

et al. 2007

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The development of a contrast agent for a laser optoacoustic imaging system (LOIS) can significantly widen preclinical and clinical applications of this imaging modality for early detection of cancerous tumors. Gold nanorods were engineered to enhance the contrast for optoacoustic imaging. Under in vivo conditions, 25 µL of gold nanorods solution at a concentration of 1.25 pM were injected into nude mice and detected using a single-channel acoustic transducer. LOIS was used to visualize the distribution of gold nanoparticles at a concentration of 125 pM in vivo when 100 µL of solution of gold nanoparticles was delivered subcutaneously. Our results suggest that LOIS can be used for in vivo detection of gold nanorods at low concentrations and the nanoparticles can be engineered to enhance the diagnostic power of optoacoustic imaging.

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“…It has been shown that PAT can depict subsurface tissue structures and functional changes noninvasively with resolution up to 100 m (14, 15). Like other optical modalities, PAT is highly sensitive in mapping and quantifying the dynamic distribution of optical contrast agents such as metallic nanocolloids and organic dyes (16)(17)(18)(19)27).…”

mentioning

confidence: 99%

Picomolar sensitivity MRI and photoacoustic imaging of cobalt nanoparticles

Bouchard

Anwar

Liu

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

171

127

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Multimodality imaging based on complementary detection principles has broad clinical applications and promises to improve the accuracy of medical diagnosis. This means that a tracer particle advantageously incorporates multiple functionalities into a single delivery vehicle. In the present work, we explore a unique combination of MRI and photoacoustic tomography (PAT) to detect picomolar concentrations of nanoparticles. The nanoconstruct consists of ferromagnetic (Co) particles coated with gold (Au) for biocompatibility and a unique shape that enables optical absorption over a broad range of frequencies. The end result is a dual-modality probe useful for the detection of trace amounts of nanoparticles in biological tissues, in which MRI provides volume detection, whereas PAT performs edge detection.dual-modality imaging ͉ ferromagnetic nanoparticle ͉ molecular imaging ͉ MRI contrast ͉ photoacoustic tomography W e have synthesized nanoparticles for dual-modality (1-7)MRI and photoacoustic tomography (PAT). The incorporation of MRI and PAT into a single probe offers the unique possibility of combining the complementary strategies of contrastbased volume imaging and edge detection. Our nanoconstruct consists of zero-valence ferromagnetic cobalt (Co) particles (8) with a gold (Au) coating for biocompatibility and a unique shape rendering increased optical absorption over a broad range of frequencies (9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19). This research theme follows the rapid developments in nanotechnology, diagnostic radiology, and targeted molecular imaging (20), whereby nanoparticulate contrast agents, with the desirable properties of high chemical specificity, biocompatibility, and a reasonable half-life, are administered within a specific region of interest. In nanoparticle-based imaging studies, higher particle concentrations lead to better signal-to-noise contrasts, but this also poses a tradeoff with the toxicity. Therefore, one of the most important parameters when developing particle-based contrast is the safest and lowest nanoparticle concentration that offers sufficient contrast sensitivity.In MRI, magnetic materials such as gadolinium chelates and magnetic nanoparticles are often used (21-23) to enhance image contrast. The magnetic nanoparticles are passivated by biocompatible coatings such as dextrin, citrate, polystyrene/divinylbenzene, and elemental gold. These coatings also detoxify the particles, resulting in enhanced lifetimes in vivo. Typical examples of magnetic nanoparticulate core-shell configurations include magnetitedextrin, magnetite-silica (24) and iron-gold (25).Laser-based PAT (9-19) is a hybrid imaging modality [see supporting information (SI) Fig. S1]. It uses a pulsed laser source to illuminate a biological sample. Light absorption by the tissue results in a transient temperature rise on the order of 10 mK. The rapid thermoelastic expansion excites ultrasonic waves that are measured by using broadband ultrasonic transducers conformally arranged around the sample. Finally, a modif...

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Bioconjugated gold nanoparticles as a molecular based contrast agent: implications for imaging of deep tumors using optoacoustic tomography

Cited by 293 publications

References 23 publications

Biomedical Applications of Plasmon Resonant Metal Nanoparticles

Biomedical Applications of Plasmon Resonant Metal Nanoparticles

High Sensitivity of In Vivo Detection of Gold Nanorods Using a Laser Optoacoustic Imaging System

Picomolar sensitivity MRI and photoacoustic imaging of cobalt nanoparticles

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