Bioconcentration and metabolism of 4-tert-octylphenol in roach (Rutilus rutilus) fry

Ferreira-Leach, A. M. R.; Hill, Elizabeth M.

doi:10.1051/analusis:2000280789

Cited by 11 publications

(4 citation statements)

References 12 publications

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“…In contrast to the extensive hydroxylation reactions of tOP observed in rudd, the major metabolite in bile from rainbow trout similarly exposed to tOP was identified as tOP-β-glucuronide, and only one minor metabolite was the product of hydroxylation, namely 4-tert-octylcatechol-β-glucuronide (28). However similar exposure of roach fry to tOP resulted in the formation of at least five metabolites, one of which was identified as tOP-β-glucuronide (48). Together, these results suggest that rudd and juvenile roach may have more extensive mechanisms for the transformation of xenobiotics than the salmonid species.…”

Section: Discussionmentioning

confidence: 84%

Tissue Distribution and Depuration of 4-tert-Octylphenol Residues in the Cyprinid Fish, Scardinius erythrophthalmus

Pedersen

Hill

2002

Environ. Sci. Technol.

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Alkylphenols are present in the aquatic environment through the degradation of alkylphenolpolyethoxylate surfactants in sewage treatment works. Branched chain 4-alkylphenols have been shown to retard testicular growth and stimulate vitellogenin synthesis in freshwater fish. We conducted in vivo studies in order to determine the fate and persistence of radiolabeled 4-tert-octylphenol (tOP) in the cyprinid fish, rudd (Scardinius erythrophthalmus). Sexually mature rudd were exposed to a concentration of 4.7 microg/L of [14C] tOP in a flow through system for 10 days. Radioactive residues were extracted from soft tissues and analyzed by radio-high-performance liquid chromatography. tOP accumulated as the major residue in muscle, ovary, and testis with bioconcentration factors of 24, 85, and 169, respectively. tOP residues in blood, gill, kidney, liver, and bile were extensively metabolized. Analysis of tOP residues in bile revealed 10 major metabolites, which were identified by GC-MS as products of aromatic and aliphatic hydroxylation, glucuronidation, and glucosidation. Depuration studies with exposed fish placed in clean water for up to 10 days resulted in a rapid loss of soluble residues from the tissues with half-lives of between 0.7 and 1.0 days (muscle, testis, ovary, gill, blood, kidney), 1.7 days (liver), and 5.9 days (bile). A further portion of radioactive residues was extracted from blood, gill, kidney, and liver after alkaline digestion, suggesting the formation of covalently bound protein adducts in these tissues. This study suggests that although para-alkyphenolic xenoestrogens can accumulate in muscle and the gonads of adult fish, residues are rapidly depurated from these tissues. Furthermore, analysis of the parent alkylphenol in bile, after hydrolysis of the conjugates, is likely to significantly underestimate the total concentration of alkylphenol residues and may not serve as an appropriate biomarker for quantifying the exposure of wild fish to alkylphenols.

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Section: Discussionmentioning

confidence: 84%

Tissue Distribution and Depuration of 4-tert-Octylphenol Residues in the Cyprinid Fish, Scardinius erythrophthalmus

Pedersen

Hill

2002

Environ. Sci. Technol.

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“…The relative lack of short chain APs (C 2 AP and C 3 AP) in the liver tissue of late juveniles supports the theory that the detoxification enzyme system is much more developed at this later stage. Generally, fish are quite efficient at metabolizing APs, mainly by phase II enzymes that conjugate intact APs to their corresponding glucuronides (Ferreira-Leach and Hill, 2000). …”

Section: Uptake Of Aps Into Fish Tissuesmentioning

confidence: 99%

Development of Atlantic cod (Gadus morhua) exposed to produced water during early life stages: Effects on embryos, larvae, and juvenile fish

Meier

Morton

Nyhammer

et al. 2010

Marine Environmental Research

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Produced water (PW) contains numerous toxic compounds of natural origin, such as dispersed oil, metals, alkylphenols (APs), and polycyclic aromatic hydrocarbons (PAHs). In addition, PW also contains many different chemicals which have been added during the oil production process. In the study described here, cod were exposed to real PW collected from an oil production platform in the North Sea. This was done in order to best recreate the most realistic field-exposure regime in which fish will be affected by a wide range of chemicals. The biological effects found in this study therefore cannot be assigned to one group of chemicals alone, but are the result of exposure to the complex chemical mixture found in real PW. Since APs are well known to cause endocrine disruption in marine organisms, we focused our chemical analysis on APs in an attempt to better understand the long-term effects of APs from PW on the biology of fish. In this study, cod were exposed to several concentrations of real PW and 17β-oestradiol (E(2)), a natural oestrogen, at different developmental stages. Cod were exposed to PW either during the embryo and early larvae stage (up to 3 months of age) or during the early juvenile stage (from 3 to 6 months of age). Results showed that, in general, APs bioconcentrate in fish tissue in a dose and developmental stage dependent manner during PW exposure. However, juveniles appeared able to effectively metabolise the short chain APs. Importantly, PW exposure had no effect on embryo survival or hatching success. However, 1% PW clearly interfered with the development of normal larval pigmentation. After hatching most of the larvae exposed to 1% PW failed to begin feeding and died of starvation. This inability to feed may be linked to the increased incidence of jaw deformities seen in these larvae. In addition, cod exposed to 1% PW, had significantly higher levels of the biomarkers vitellogenin and CYP1A in plasma and liver, respectively. No similar effects were seen in cod exposed to either 0.1% or 0.01% PW.

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“…Low concentrations of EDCs were also recorded in pore water collected right after the exposure period ended (Table 3). In laboratory controlled uptake studies for NP, Bioconcentration factors (BCFs) yielded ranged from 200 to 300 [19,20] whereas, OP were recorded to be from 250 to 350 [21,22]. If P. yokohamae in this study has bio accumulated the target compound from water, the estimated concentration of NP and OP from maximum value would be approximately 2,300 and 221 ng/g w.w., respectively.…”

Section: Results and Discussion Water Parameters And Control Sedimentmentioning

confidence: 78%

BIOACCUMULATION OF SEDIMENTARY ENDOCRINE DISRUPTING CHEMICALS (EDCs) BY THE BENTHIC FISH, Pleuronectes yokohamae

2017

MJAS

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In this study, Pleuronectes yokohamae (P. yokohamae) were exposed to the sediment mixed with mixture of nonylphenol (NP), octylphenol (OP), estrone (E1) and 17β-estradiol (E2) which makes up Endocrine Disrupting Chemicals (EDCs). The target compound was detected in water sample, and NP and OP concentrations were found to be the highest, 7.66 μg/L and 0.63 μg/L respectively (E1 and E2 concentrations were below the limit of detection). In pore water on the other hand, NP concentrations recorded the highest with 35.3 μg/L, while concentrations of OP, E1 and E2 were 4.08 μg/L, 0.06 μg/L and 0.18 μg/L respectively. P. yokohamae shown rapid and maximum accumulation of NP and OP on day 3, where NP recorded to be 2200 ng/g d.w. and OP 168 ng/g d.w. The recorded BAF values in low and high exposure groups were 0.010 and 0.065 for NP, and 0.065 and 0.084 for OP. The BAF findings recorded, being less than 1 indicated that there were no bioaccumulations in the tested fish of both groups. However, due to their nature of being more predisposed to accumulating EDCs than the water column, the bioaccumulation of contaminant in benthic fish should be continually monitored.

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Bioconcentration and metabolism of 4-tert-octylphenol in roach (Rutilus rutilus) fry

Cited by 11 publications

References 12 publications

Tissue Distribution and Depuration of 4-tert-Octylphenol Residues in the Cyprinid Fish, Scardinius erythrophthalmus

Tissue Distribution and Depuration of 4-tert-Octylphenol Residues in the Cyprinid Fish, Scardinius erythrophthalmus

Development of Atlantic cod (Gadus morhua) exposed to produced water during early life stages: Effects on embryos, larvae, and juvenile fish

BIOACCUMULATION OF SEDIMENTARY ENDOCRINE DISRUPTING CHEMICALS (EDCs) BY THE BENTHIC FISH, Pleuronectes yokohamae

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