Biocompatible TiO2 nanoparticle-based cell immunoassay for circulating tumor cells capture and identification from cancer patients

Abstract

We demonstrate the isolation of circulating tumor cells (CTCs) with a biocompatible nano-film composed of TiO2 nanoparticles. Due to the enhanced topographic interaction between nano-film and cancer cell surface, cancer cells (HCT116) spiked into PBS and healthy blood can be recovered from the suspension, whose efficiencies were respectively 80 % and 50 %. Benifit from the biocompatibility of this nano-film, in-situ culture of the captured cancer cells is also available, which provides an alternative selection when the capture cell number was inadequate or the sample cannot be analyzed immediately. For the proof-of-concept study, we use this nano-film to separate the circulating tumor cells from the colorectal and gastric cancer patient peripheral blood samples and the captured CTCs are identified by a three-colored immunocytochemistry method. We investigated the cancer cells capture strength at the nano-bio interface through exposing the cells to fluid shear stress in microfluidic device, which can be utilized to increase the purity of CTCs. The result indicated that 50 % of the captured cells can be detached from the substrate when the fluid shear stress was 180 dyn cm(-2). By integration of this CTCs capture nano-film with other single cell analysis device, we expected to further explore their applications in genome sequencing based on the captured CTCs.

Mechanisms of Ion Detection for FET-Sensors Using FTO: Role of Cleaning Process, pH Sequence and Electrical Resistivity

“…Oxides such as TiO 2 2 , SnO 2 3 and ZnO 1 can be used as sensing part in these devices. The biocompatibility of these materials has already been demonstrated by other research groups [4][5][6][7][8] , and they have been used as part of various types of biosensors.…”

Transparent, biocompatible nanostructured surfaces for cancer cell capture and culture

“…Captured cells were then stained with PE-labeled anti-CK, FITC-labeled anti-CD45 and DAPI. [26][27][28][29] Fluorescence microscopy was used to distinguish specifically captured CTCs (CK+/CD45-/DAPI+) from non-specifically immobilized WBCs (CK-/CD45+/DAPI+) by their fluorescence and larger size (CTCs: 10-30 µm; 1 WBCs, 15 µm) ( Figure 6A). CTC enumeration results are summarized in Figure 6B and Table 1.…”

Aptamer-Mediated Transparent-Biocompatible Nanostructured Surfaces for Hepatocellular Circulating Tumor Cells Enrichment

“…And, we also compared the capture performance of anti-EpCAM coated CTC- BioT Chip with slex-AP coated CTC- BioT Chip using artificial blood (detailed in supporting information). PE-labeled anti-CK, FITC-labeled anti-CD45 and DAPI were introduced onto the CTC- BioT Chip for artificial CTCs' identification 24 (Figure 3E). These results indicate that sLeX-AP coated CTC- BioT Chip exhibited sufficient capture efficiency to manage CTCs capture and identification from clinical samples which normally had a few to hundreds CTCs mL -1 .…”