Biocompatible polymer enhances the <i>in vitro</i> and <i>in vivo</i> transfection efficiency of HVJ envelope vector

Mima, Hidetoshi; Tomoshige, Ryuji; Kanamori, Tatsuyuki; Tabata, Yasuhiko; Yamamoto, Seiji; Ito, Susumu; Tamai, Katsuto; Kaneda, Yasufumi

doi:10.1002/jgm.735

Cited by 30 publications

(28 citation statements)

References 52 publications

(52 reference statements)

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“…However, we previously showed that CG-HVJ-E is remarkably stable in 50% fresh mouse serum. Although it is unproven that HVJ is degraded by complement lysis in mouse serum, as known to be the case for retrovirus and HIV (31,32), the interaction between serum proteins and HVJ-E may contribute to reduced transfection efficacy of HVJ-E. Conjugation with CG seems to protect the surface molecules of HVJ-E from the detrimental effects of serum proteins (24). In this report, CG-HVJ-E showed a proclivity toward i.p.…”

Section: Discussionmentioning

confidence: 62%

“…Preparation of CG-HVJ-E/Bleomycin, Polymer-Conjugated HVJ-E, and CG/Bleomycin Cationization of gelatin was done by introducing ethylene diamine into the carboxyl groups of low molecular weight gelatin (MW = 5,000), as previously described (24), and the mole-to-mole ratio of amino groups to carboxyl groups within the gelatin was 48.7. A 5-mg amount of CG was added to 300 AL of PBS containing 3 Â 10 10 particles of HVJ-E vector containing bleomycin.…”

Section: Methodsmentioning

confidence: 99%

“…For the evaluation of fusion-mediated delivery, we used antiserum against the F protein of HVJ prepared in our laboratory by immunizing a rabbit with purified F protein (24). The concentration of anti-F antibodies in the antiserum was f30 Ag/mL.…”

Section: Assessment Of the Effect Of Fusion And Endocytosis On Hvj-e mentioning

confidence: 99%

“…To enhance drug delivery, we combined HVJ-E with cationized gelatin (CG; ref. 24). CG-conjugated HVJ-E (CG-HVJ-E) was still capable of fusion.…”

Section: Introductionmentioning

confidence: 99%

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Targeted chemotherapy against intraperitoneally disseminated colon carcinoma using a cationized gelatin–conjugated HVJ envelope vector

Mima

Yamamoto

Itô

et al. 2006

Molecular Cancer Therapeutics

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The hemagglutinating virus of Japan envelope (HVJ-E; Sendai virus) vector derived from inactivated HVJ particles can be used to deliver DNA, proteins, and drugs into cells both in vitro and in vivo. HVJ-E is capable of delivering bleomycin, an anticancer drug, to various cancer cell lines, thereby producing 300-fold greater cytotoxicity than administration of bleomycin alone. In a mouse model of peritoneally disseminated colon cancer, we injected HVJ-E containing the luciferase gene into the peritoneum.

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Section: Discussionmentioning

confidence: 62%

Section: Methodsmentioning

confidence: 99%

Section: Assessment Of the Effect Of Fusion And Endocytosis On Hvj-e mentioning

confidence: 99%

“…To enhance drug delivery, we combined HVJ-E with cationized gelatin (CG; ref. 24). CG-conjugated HVJ-E (CG-HVJ-E) was still capable of fusion.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Targeted chemotherapy against intraperitoneally disseminated colon carcinoma using a cationized gelatin–conjugated HVJ envelope vector

Mima

Yamamoto

Itô

et al. 2006

Molecular Cancer Therapeutics

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“…39,41 With HVJ-E, membrane fusion occurs at the cell surface, not in endosomes. 22 The fact that gene delivery by HVJ-E is not affected by inhibitors of endocytosis 51,52 indicates that the viral genome can be directly delivered to the cytoplasm. Therefore, we theorize that IFN production by HVJ-E is also mediated by RIG-I in the cytoplasm, not by TLR3, which is present on the endosomal membrane.…”

Section: Discussionmentioning

confidence: 99%

Efficient eradication of hormone‐resistant human prostate cancers by inactivated Sendai virus particle

Kawaguchi

Miyamoto

Inoue

et al. 2009

Intl Journal of Cancer

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138

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Hormone-refractory prostate cancer is one of the intractable human cancers in the world. Here, we examined the direct tumorkilling activity of inactivated Sendai virus particle [hemagglutinating virus of Japan envelope (HVJ-E)] through induction of Type I interferon (IFN) in the hormone-resistant human prostate cancer cell lines PC3 and DU145. Preferential binding of HVJ-E to PC3 and DU145 over hormone-sensitive prostate cancer cell and normal prostate epithelium was observed, resulting in a number of fused cells. After HVJ-E treatment, a number of IFNrelated genes were up-regulated, resulting in Type I IFN production in PC3 cells. Then, retinoic acid-inducible gene-I (RIG-I) helicase which activates Type I IFN expression after Sendai virus infection was up-regulated in cancer cells after HVJ-E treatment. Produced IFN-a and -b enhanced caspase 8 expression via Janus kinases/Signal Transducers and Activators of Transcription pathway, activated caspase 3 and induced apoptosis in cancer cells. When HVJ-E was directly injected into a mass of PC3 tumor cells in SCID (severe combined immunodeficiency) mice, a marked reduction in the bulk of each tumor mass was observed and 85% of the mice became tumor-free. Although co-injection of an antiasialo GM1 antibody with HVJ-E into each tumor mass slightly attenuated the tumor suppressive activity of HVJ-E, significant suppression of tumor growth was observed even in the presence of anti-asialo GM1 antibody. This suggests that natural killer cell activation made small contribution to tumor regression following HVJ-E treatment in hormone-resistant prostate cancer model in vivo. Thus, HVJ-E effectively targets hormone-resistant prostate cancer by inducing apoptosis in tumor cells, as well as activating anti-tumor immunity. '

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Subcellular Targeting of Virus‐Envelope‐Coated Nanoparticles

Wang

Saeed

Kolokoltsov

et al. 2010

Organelle‐Specific Pharmaceutical Nanotechnology

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Biocompatible polymer enhances the in vitro and in vivo transfection efficiency of HVJ envelope vector

Abstract: Cationized gelatin-conjugated HVJ-E enhanced gene transfection efficiency both in vitro and in vivo. These results suggest that low molecular weight cationized gelatin may be appropriate for complex formation with various envelope viruses, such as retrovirus, herpes virus and HIV.

Cited by 30 publications

References 52 publications

Targeted chemotherapy against intraperitoneally disseminated colon carcinoma using a cationized gelatin–conjugated HVJ envelope vector

Targeted chemotherapy against intraperitoneally disseminated colon carcinoma using a cationized gelatin–conjugated HVJ envelope vector

Efficient eradication of hormone‐resistant human prostate cancers by inactivated Sendai virus particle

Subcellular Targeting of Virus‐Envelope‐Coated Nanoparticles

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