Biocompatible Organic Coatings Based on Bisphosphonic Acid RGD-Derivatives for PEO-Modified Titanium Implants

Parfenova, Lyudmila V.; Lukina, E. S.; Galimshina, Zulfiya R.; Gil’fanova, Guzel U.; Mukaeva, V. R.; Фаррахов, Р. Г.; Данилко, К. В.; D’yakonov, G. S.; Парфенов, Е.В.

doi:10.3390/molecules25010229

Cited by 23 publications

(11 citation statements)

References 86 publications

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“…Cell differentiation is a critical factor for bone development. , Numerous studies have revealed that PEO treatment on titanium enhances osteogenic differentiation. ,,, Our results also showed that PEO-treated Ti-6Al-4V-Ca 2+ /P i significantly promoted osteogenic differentiation of MC3T3-E1 cells by elevating the expression of bone differentiation markers such as alkaline phosphatase (ALP), osteopontin (OPN), type I collagen (Col1A1), and osteocalcin (OCN). ALP activity and mineralization in cells cultured on PEO-treated Ti-6Al-4V-Ca 2+ /P i surfaces were markedly higher than on the untreated Ti-6Al-4V surface.…”

Section: Discussionsupporting

confidence: 60%

DMP1 and IFITM5 Regulate Osteogenic Differentiation of MC3T3-E1 on PEO-Treated Ti-6Al-4V-Ca²⁺/P_i surface

Jeong

Nguyen

et al. 2023

ACS Biomater. Sci. Eng.

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Despite numerous studies on various surface modifications on titanium and its alloys, it remains unclear what kind of titanium-based surface modifications are capable of controlling cell activity. This study aimed to understand the mechanism at the cellular and molecular levels and investigate the in vitro response of osteoblastic MC3T3-E1 cultured on the Ti-6Al-4V surface modified by plasma electrolytic oxidation (PEO) treatment. A Ti-6Al-4V surface was prepared by PEO at 180, 280, and 380 V for 3 or 10 min in an electrolyte containing Ca 2+ /P i ions. Our results showed that PEO-treated Ti-6Al-4V-Ca 2+ /P i surfaces enhanced the cell attachment and differentiation of MC3T3-E1 compared to the untreated Ti-6Al-4V control but did not affect cytotoxicity as shown by cell proliferation and cell death. Interestingly, on the Ti-6Al-4V-Ca 2+ /P i surface treated by PEO at 280 V for 3 or 10 min, MC3T3-E1 showed a higher initial adhesion and mineralization. In addition, the alkaline phosphatase (ALP) activity significantly increased in MC3T3-E1 on the PEO-treated Ti-6Al-4V-Ca 2+ /P i (280 V for 3 or 10 min). In RNA-seq analysis, the expression of dentin matrix protein 1 (DMP1), sortilin 1 (Sort1), signal-induced proliferation-associated 1 like 2 (SIPA1L2), and interferon-induced transmembrane protein 5 (IFITM5) was induced during the osteogenic differentiation of MC3T3-E1 on the PEO-treated Ti-6Al-4V-Ca 2+ /P i . DMP1 and IFITM5 silencing decreased the expression of bone differentiation-related mRNAs and proteins and ALP activity in MC3T3-E1. These results suggest that the PEO-treated Ti-6Al-4V-Ca 2+ /P i surface induces osteoblast differentiation by regulating the expression of DMP1 and IFITM5. Therefore, surface microstructure modification through PEO coatings with Ca 2+ /P i ions could be used as a valuable method to improve biocompatibility properties of titanium alloys.

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Section: Discussionsupporting

confidence: 60%

DMP1 and IFITM5 Regulate Osteogenic Differentiation of MC3T3-E1 on PEO-Treated Ti-6Al-4V-Ca²⁺/P_i surface

Jeong

Nguyen

et al. 2023

ACS Biomater. Sci. Eng.

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“…RGD stimulates bone integration by increasing the proliferation and adhesion of osteoblasts on the implant surface ( Elmengaard et al, 2005 ). One study reported that the hybrid molecular coating obtained by chemical crosslinking of amino acid bisphosphonate and linear tripeptide RGD combines, which has a strong ability to stimulate bone integration ( Parfenova et al, 2020 ). Furthermore, another study showed that identifying the exons of hMSC osteogenic differentiation and binding them to the implant surface can achieve acellular bone regeneration and the bone tissue regeneration efficiency of acellular coated implants was equivalent to hMSCs-implanted acellular implants ( Figure 7 ) ( Zhai et al, 2020 ).…”

Section: Functionalization Of the Implant Surfacementioning

confidence: 99%

Advanced Surface Modification for 3D-Printed Titanium Alloy Implant Interface Functionalization

Sheng

Wang

et al. 2022

Front. Bioeng. Biotechnol.

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With the development of three-dimensional (3D) printed technology, 3D printed alloy implants, especially titanium alloy, play a critical role in biomedical fields such as orthopedics and dentistry. However, untreated titanium alloy implants always possess a bioinert surface that prevents the interface osseointegration, which is necessary to perform surface modification to enhance its biological functions. In this article, we discuss the principles and processes of chemical, physical, and biological surface modification technologies on 3D printed titanium alloy implants in detail. Furthermore, the challenges on antibacterial, osteogenesis, and mechanical properties of 3D-printed titanium alloy implants by surface modification are summarized. Future research studies, including the combination of multiple modification technologies or the coordination of the structure and composition of the composite coating are also present. This review provides leading-edge functionalization strategies of the 3D printed titanium alloy implants.

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“…As previously shown, cp-Ti surface biofunctionalization with a titania PEO coating and organic molecules appears to be more effective than the formation of Ca- and P- containing inorganic PEO coatings [ 22 ]. Therefore, this research focuses on a titania PEO coating for cp-Ti as a substrate for further modification with the bioactive organic molecules addressing the biomimetic requirements at the biological level [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Investigation of Biocompatible PEO Coating Growth on cp-Ti with In Situ Spectroscopic Methods

et al. 2021

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The problem of the optimization of properties for biocompatible coatings as functional materials requires in-depth understanding of the coating formation processes; this allows for precise manufacturing of new generation implantable devices. Plasma electrolytic oxidation (PEO) opens the possibility for the design of biomimetic surfaces for better biocompatibility of titanium materials. The pulsed bipolar PEO process of cp-Ti under voltage control was investigated using joint analysis of the surface characterization and by in situ methods of impedance spectroscopy and optical emission spectroscopy. Scanning electron microscopy, X-ray diffractometry, coating thickness, and roughness measurements were used to characterize the surface morphology evolution during the treatment for 5 min. In situ impedance spectroscopy facilitated the evaluation of the PEO process frequency response and proposed the underlying equivalent circuit where parameters were correlated with the coating layer properties. In situ optical emission spectroscopy helped to analyze the spectral line evolutions for the substrate material and electrolyte species and to justify a method to estimate the coating thickness via the relation of the spectral line intensities. As a result, the optimal treatment time was established as 2 min; this provides a 9–11 µm thick PEO coating with Ra 1 µm, 3–5% porosity, and containing 75% of anatase. The methods for in-situ spectral diagnostics of the coating thickness and roughness were justified so that the treatment time can be corrected online when the coating achieves the required properties.

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Biocompatible Organic Coatings Based on Bisphosphonic Acid RGD-Derivatives for PEO-Modified Titanium Implants

Cited by 23 publications

References 86 publications

DMP1 and IFITM5 Regulate Osteogenic Differentiation of MC3T3-E1 on PEO-Treated Ti-6Al-4V-Ca²⁺/P_i surface

DMP1 and IFITM5 Regulate Osteogenic Differentiation of MC3T3-E1 on PEO-Treated Ti-6Al-4V-Ca²⁺/P_i surface

Advanced Surface Modification for 3D-Printed Titanium Alloy Implant Interface Functionalization

Investigation of Biocompatible PEO Coating Growth on cp-Ti with In Situ Spectroscopic Methods

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Biocompatible Organic Coatings Based on Bisphosphonic Acid RGD-Derivatives for PEO-Modified Titanium Implants

Cited by 23 publications

References 86 publications

DMP1 and IFITM5 Regulate Osteogenic Differentiation of MC3T3-E1 on PEO-Treated Ti-6Al-4V-Ca2+/Pi surface

DMP1 and IFITM5 Regulate Osteogenic Differentiation of MC3T3-E1 on PEO-Treated Ti-6Al-4V-Ca2+/Pi surface

Advanced Surface Modification for 3D-Printed Titanium Alloy Implant Interface Functionalization

Investigation of Biocompatible PEO Coating Growth on cp-Ti with In Situ Spectroscopic Methods

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DMP1 and IFITM5 Regulate Osteogenic Differentiation of MC3T3-E1 on PEO-Treated Ti-6Al-4V-Ca²⁺/P_i surface

DMP1 and IFITM5 Regulate Osteogenic Differentiation of MC3T3-E1 on PEO-Treated Ti-6Al-4V-Ca²⁺/P_i surface