Biocompatible Nanocomposite Enhanced Osteogenic and Cementogenic Differentiation of Periodontal Ligament Stem Cells In Vitro for Periodontal Regeneration

Jin, Liu; Dai, Quan; Weir, Michael D.; Schneider, Abraham; Zhang, Charles; Hack, Gary D.; Oates, Thomas W.; Zhang, Ke; Li, Ang; Xu, Hockin H.K.

doi:10.3390/ma13214951

Cited by 14 publications

(8 citation statements)

References 64 publications

(75 reference statements)

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“…However, low expressions of the hematopoietic stem cell surface markers CD31 (0.76%), CD34 (2.01%), and CD45 (0.11%) were observed ( Figure 2B ). All of the results suggest that the isolated hPDLSCs had the potential for osteogenic differentiation and were not mixed with hematopoietic stem cells or endothelial cells ( Liu et al, 2020 ; Peng et al, 2021 ; Rao et al, 2021 ).…”

Section: Resultsmentioning

confidence: 92%

Biomimetic Design and Fabrication of Sericin-Hydroxyapatite Based Membranes With Osteogenic Activity for Periodontal Tissue Regeneration

Ming

Rao

et al. 2022

Front. Bioeng. Biotechnol.

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The guided tissue regeneration (GTR) technique is a promising treatment for periodontal tissue defects. GTR membranes build a mechanical barrier to control the ingrowth of the gingival epithelium and provide appropriate space for the regeneration of periodontal tissues, particularly alveolar bone. However, the existing GTR membranes only serve as barriers and lack the biological activity to induce alveolar bone regeneration. In this study, sericin-hydroxyapatite (Ser-HAP) composite nanomaterials were fabricated using a biomimetic mineralization method with sericin as an organic template. The mineralized Ser-HAP showed excellent biocompatibility and promoted the osteogenic differentiation of human periodontal membrane stem cells (hPDLSCs). Ser-HAP was combined with PVA using the freeze/thaw method to form PVA/Ser-HAP membranes. Further studies confirmed that PVA/Ser-HAP membranes do not affect the viability of hPDLSCs. Moreover, alkaline phosphatase (ALP) staining, alizarin red staining (ARS), and RT-qPCR detection revealed that PVA/Ser-HAP membranes induce the osteogenic differentiation of hPDLSCs by activating the expression of osteoblast-related genes, including ALP, Runx2, OCN, and OPN. The unique GTR membrane based on Ser-HAP induces the differentiation of hPDLSCs into osteoblasts without additional inducers, demonstrating the excellent potential for periodontal regeneration therapy.

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Section: Resultsmentioning

confidence: 92%

Biomimetic Design and Fabrication of Sericin-Hydroxyapatite Based Membranes With Osteogenic Activity for Periodontal Tissue Regeneration

Ming

Rao

et al. 2022

Front. Bioeng. Biotechnol.

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“…2 B). Mineralization-related proteins or genes (Runx2, Osterix, Col1, Alp, Cap, and Cemp) were chosen based on previous research [ 32 , 33 ], Additionally, WB demonstrated that CTGF enhanced the protein abundance of Runx2, Osterix, Col1, and Cap in a concentration-dependent manner (Fig. 2 C), and the abundance of these targeted proteins was about twice that of the untreated group (Fig.…”

Section: Resultsmentioning

confidence: 99%

Connective tissue growth factor promotes cementogenesis and cementum repair via Cx43/β-catenin axis

Chen

et al. 2022

Stem Cell Res Ther

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Background Orthodontic tooth movement inevitably induces cementum resorption, which is an urgent problem for orthodontists to confront. Human periodontal ligament stem cells (hPDLSCs) exert an important role in the orthodontic tooth movement and exhibit multidirectional differentiation ability in cementum regeneration. Connective tissue growth factor (CTGF) is an important extracellular matrix protein for bone homeostasis and cell differentiation. The purpose of our study was to explore the role of CTGF in cementum repair and cementogenesis and to elucidate its underlying mechanism. Methods A cementum defect model was established by tooth movement with heavy forces, and the cementum repair effect of CTGF was observed via micro-CT, HE staining and immunohistochemical staining. RT‒qPCR, western blotting (WB), alizarin red staining and ALP activity experiments verified the mineralization ability of hPDLSCs stimulated with CTGF. The expression of Cx43 in periodontal ligament cells was detected by WB and immunofluorescence (IF) experiments after CTGF stimulation in vivo and in vitro. Subsequently, the mineralization ability of hPDLSCs was observed after application of CTGF and the small interfering RNA Si-Cx43. Additionally, co-intervention via application of the small interfering RNA Si-CTGF and the Cx43 agonist ATRA in hPDLSCs was performed to deepen the mechanistic study. Next, WB, IF experiments and co-immunoprecipitation were conducted to confirm whether CTGF triggers the Cx43/β-catenin axis to regulate cementoblast differentiation of hPDLSCs. Results Local oral administration of CTGF to the cementum defects in vivo facilitated cementum repair. CTGF facilitated the cementogenesis of hPDLSCs in a concentration-dependent manner. Cx43 acted as a downstream effector of CTGF to regulate cementoblast differentiation. Si-Cx43 reduced CTGF-induced cementoblast differentiation. The Cx43 agonist ATRA restored the low differentiation capacity induced by Si-CTGF. Further mechanistic studies showed that CTGF triggered the activation of β-catenin in a dose-dependent manner. In addition, co-localization IF analysis and co-immunoprecipitation demonstrated that Cx43 interacted with β-catenin at cell‒cell connections. Si-Cx43 attenuated the substantial expression of β-catenin induced by CTGF. The Cx43 agonist reversed the inhibition of β-catenin induced by Si-CTGF. IF demonstrated that the nuclear importation of β-catenin was related to the immense expression of Cx43 at cell‒cell junctions. Conclusions Taken together, these data demonstrate that CTGF promotes cementum repair and cementogenesis through activation of the Cx43/β-catenin signalling axis.

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“…Periodontal embryonic stem cells are the most common cells in the tissue engineering of periodontitis treatment because of the ability to differentiate into osteoblasts, odontogenic osteocytes, fibroblasts and subsequently form the corresponding periodontal tissues. In addition, growth factors, such as basic fibroblast growth factor (bFGF) [ 178 ], epidermal growth factor (EGF) [ 179 ], BMP [ 180 ], and VEGF [ 181 ], can promote the differentiation of seed cells, are also widely applied in the tissue engineering of periodontitis treatment. In this part, we will further introduce the types and design strategies of nanoscaffold for periodontal tissue engineering.…”

Section: Periodontal Diseasesmentioning

confidence: 99%

Advanced materials and technologies for oral diseases

Cui

You

Cheng

et al. 2023

Science and Technology of Advanced Materials

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Oral disease, as a class of diseases with very high morbidity, brings great physical and mental damage to people worldwide. The increasing burden and strain on individuals and society make oral diseases an urgent global health problem. Since the treatment of almost all oral diseases relies on materials, the rapid development of advanced materials and technologies has also promoted innovations in the treatment methods and strategies of oral diseases. In this review, we systematically summarized the application strategies in advanced materials and technologies for oral diseases according to the etiology of the diseases and the comparison of new and old materials. Finally, the challenges and directions of future development for advanced materials and technologies in the treatment of oral diseases were refined. This review will guide the fundamental research and clinical translation of oral diseases for practitioners of oral medicine.

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Biocompatible Nanocomposite Enhanced Osteogenic and Cementogenic Differentiation of Periodontal Ligament Stem Cells In Vitro for Periodontal Regeneration

Cited by 14 publications

References 64 publications

Biomimetic Design and Fabrication of Sericin-Hydroxyapatite Based Membranes With Osteogenic Activity for Periodontal Tissue Regeneration

Biomimetic Design and Fabrication of Sericin-Hydroxyapatite Based Membranes With Osteogenic Activity for Periodontal Tissue Regeneration

Connective tissue growth factor promotes cementogenesis and cementum repair via Cx43/β-catenin axis

Advanced materials and technologies for oral diseases

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