Biocompatible Micropatterning of Two Different Cell Types

Co, Carlos C.; Wang, Yu-Chi; Ho, Chia‐Chi

doi:10.1021/ja044382a

Cited by 94 publications

(47 citation statements)

References 12 publications

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“…The presence of 10T1/2 did not disrupt the alignment of the C2C12 myotubes, which showed that cell alignment is possible even when two different cell types are present. Several studies have revealed that cell patterning is possible even when two types of cells are present in a culture (Bhatia et al, 1997;Co et al, 2005), but cell alignment under co-culture conditions has not been reported to date. Cell alignment under heterogeneous conditions is especially important in an in vivo environment in which multiple types of cells are present but cell alignment must be maintained.…”

Section: Discussionmentioning

confidence: 99%

Application of a cell sheet–polymer film complex with temperature sensitivity for increased mechanical strength and cell alignment capability

Fujita¹,

Shimizu²,

Nagamori³

2009

Biotech & Bioengineering

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We have succeeded in fabricating a cell sheet-polymer film complex involving a temperature-sensitive polymer that has enough mechanical strength that can be manipulated even by forceps. The polymer film can be removed by lowering the temperature after transplantation, demonstrating its potential use in regenerative medicine. Recently, tissue engineering involving cell sheets was developed, tissues being fabricated by layering of these cell sheets. This technique promises high density cell packing, which is important for native cell functions, and successful heart therapy using cardiac cell sheets has been reported. On the other hand, the fabrication of a large tissue using cell sheets is difficult because of fragility of the cell sheets. Here, we have developed a novel method in which cells are attached to a temperature-sensitive poly-N-isopropylacrylamide film mixed with laminin and collagen IV, and report that the cell sheet-polymer film complex can be manipulated with forceps. A cell sheet can be removed from the polymer film by lowering the temperature after the manipulation. We have utilized this technique for the primary myocardium and fabricated a physiologically active multi-layered cardiac cell sheet. By applying a micropattern to this polymer film, we have succeeded in making a skeletal muscle cell sheet in which myotubes are oriented in the desired direction. Overall, we showed that this method is useful for cell sheet manipulation, morphogenesis, and transplantation.

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Section: Discussionmentioning

confidence: 99%

Application of a cell sheet–polymer film complex with temperature sensitivity for increased mechanical strength and cell alignment capability

Fujita¹,

Shimizu²,

Nagamori³

2009

Biotech & Bioengineering

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“…By 72 h in culture, these ECs formed extensive cell-cell contact, and linear cellular cords that had hollow central lumen extending several cell lengths. In another study, cell-resistant anionic copolymer of oligoethyleneglycol methacrylate and methacrylic acid was micropatterned on chitosan and gelatin via microcontact printing method, leaving 20 μm wide ECM lines for cell adhesion [59]. Human microvascular ECs cultured on these substrates formed capillary tube-like structures after 5 days.…”

Section: Micropatterning Techniques To Regulate Angiogenesis-micropatmentioning

confidence: 99%

Vascularization of Engineered Tissues: Approaches to Promote Angiogenesis in Biomaterials

Moon¹,

West²

2008

CTMC

204

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Although there have been extensive research efforts to create functional tissues and organs, most successes in tissue engineering have been limited to avascular or thin tissues. The major hurdle in development of more complex tissues lies in the formation of vascular networks capable of delivering oxygen and nutrients throughout the engineered constructs. Sufficient neovascularization in scaffold materials can be achieved through coordinated application of angiogenic factors with proper cell types in biomaterials. This review present the current research developments in the design of biomaterials and their biochemical and biochemical modifications to produce vascularized tissue constructs.

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“…They 1) typically require adhesive proteins for cell attachment, [25] 2) are limited to specific parallel geometries defined by laminar flow patterns, [26] or 3) have been used predominantly with cell lines rather than primary cells due to the difficulties involved in attaching primary cells onto synthetic surfaces. [27][28][29][30][31] PEMs have been shown to be excellent candidates for biomaterial applications [31][32][33][34][35][36][37][38][39] and provide flexibility in building complex three-dimensional architectures. [40] The PEM surfaces also provide an ability to control the arrangement of multiple cell types with subcellular resolution.…”

Section: Introductionmentioning

confidence: 99%

Primary Neuron/Astrocyte Co‐Culture on Polyelectrolyte Multilayer Films: A Template for Studying Astrocyte‐Mediated Oxidative Stress in Neurons

Kidambi

Lee

Chan

2008

Adv Funct Materials

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We engineered patterned co-cultures of primary neurons and astrocytes on polyelectrolyte multilayer (PEM) films without the aid of adhesive proteins/ligands to study the oxidative stress mediated by astrocytes on neuronal cells. A number of studies have explored engineering coculture of neurons and astrocytes predominantly using cell lines rather than primary cells owing to the difficulties involved in attaching primary cells onto synthetic surfaces. To our knowledge this is the first demonstration of patterned co-culture of primary neurons and astrocytes for studying neuronal metabolism. In our study, we used synthetic polymers, namely poly(diallyldimethylammoniumchloride) (PDAC) and sulfonated poly(styrene) (SPS) as the polycation and polyanion, respectively, to build the multilayers. Primary neurons attached and spread preferentially on SPS surfaces, while primary astrocytes attached to both SPS and PDAC surfaces. SPS patterns were formed on PEM surfaces, either by microcontact printing SPS onto PDAC surfaces or vice-versa, to obtain patterns of primary neurons and patterned co-cultures of primary neurons and astrocytes. We further used the patterned co-culture system to study the neuronal response to elevated levels of free fatty acids as compared to the response in separated monoculture by measuring the level of reactive oxygen species (ROS; a widely accepted marker of oxidative stress). The elevation in the ROS levels was observed to occur earlier in the patterned co-culture system than in the separated monoculture system. The results suggest that this technique may provide a useful tool for engineering neuronal co-culture systems, that may more accurately capture neuronal function and metabolism, and thus could be used to obtain valuable

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Biocompatible Micropatterning of Two Different Cell Types

Cited by 94 publications

References 12 publications

Application of a cell sheet–polymer film complex with temperature sensitivity for increased mechanical strength and cell alignment capability

Application of a cell sheet–polymer film complex with temperature sensitivity for increased mechanical strength and cell alignment capability

Vascularization of Engineered Tissues: Approaches to Promote Angiogenesis in Biomaterials

Primary Neuron/Astrocyte Co‐Culture on Polyelectrolyte Multilayer Films: A Template for Studying Astrocyte‐Mediated Oxidative Stress in Neurons

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