Biocompatible hyaluronic acid polymer-coated quantum dots for CD44+ cancer cell-targeted imaging

Wang, Hening; Sun, Hai; Wei, Hui; Xi, Peng; Nie, Shuming; Ren, Qiushi

doi:10.1007/s11051-014-2621-x

Cited by 16 publications

(8 citation statements)

References 56 publications

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“…113,114 As shown in Figure 10, the MTT assay did not show any statistically significant difference ( p > 0.05) in HCEC viability with exposure to AcrpHEMA and HA-grafted pHEMA hydrogel discs for 24 h, when compared to control pHEMA discs, suggesting that the washing steps upon surface acrylation and thiol-ene reaction successfully removed any unreacted chemical components and by-products. Consistent with the current results, previous studies showed that materials involving thiolated HA (HA-SH) and its derivatives or TCEP mediated thiol-ene reactions exhibited little or no cytotoxic effects in vitro 71,73,115 and in vivo. 72,116–119 Hence, the modified materials are considered to exhibit acceptable compatibility to HCEC.…”

Section: Resultssupporting

confidence: 92%

“…The quantification of the free thiols of HA-SH was achieved spectrophotometrically, using Ellman's reagent (5,5 0 -dithiobis (2-nitrobenzoic acid), DTNB). 73 Briefly, unmodified HA, HA-SS-R, and HA-SH were dissolved in sodium phosphate buffer (0.1 M, pH 8) containing 1 mM ethylenediaminetetraacetic acid (EDTA), while L-cysteine was used for the calibration curve (2-20 nmol, R 2 ¼ 0.9987). Samples and L-cysteine standards were mixed with a solution of Ellman's reagent and incubated for 15 min at room temperature under dark conditions, at which time the absorbance was measured at 412 nm using a UV-vis spectrophotometer (Spectramax Plus 384, Molecular Devices Corp., Sunnyvale, CA).…”

Section: Ellman's Test-quantification Of Free Thiols Of Ha-shmentioning

confidence: 99%

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Surface modification of model hydrogel contact lenses with hyaluronic acid via thiol-ene “click” chemistry for enhancing surface characteristics

2017

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Discontinuation of contact lens wear as a result of ocular dryness and discomfort is extremely common; as many as 26% of contact lens wearers discontinue use within the first year. While patients are generally satisfied with conventional hydrogel lenses, improving on-eye comfort continues to remain a goal. Surface modification with a biomimetic, ocular friendly hydrophilic layer of a wetting agent is hypothesized to improve the interfacial interactions of the contact lens with the ocular surface. In this work, the synthesis and characterization of poly(2-hydroxyethyl methacrylate) surfaces grafted with a hydrophilic layer of hyaluronic acid are described. The immobilization reaction involved the covalent attachment of thiolated hyaluronic acid (20 kDa) on acrylated poly(2-hydroxyethyl methacrylate) via nucleophile-initiated Michael addition thiol-ene "click" chemistry. The surface chemistry of the modified surfaces was analyzed by Fourier transform infrared spectroscopy-attenuated total reflectance and X-ray photoelectron spectroscopy. The appearance of N (1s) and S (2p) peaks on the low resolution X-ray photoelectron spectroscopy spectra confirmed successful immobilization of hyaluronic acid. Grafting hyaluronic acid to the poly(2-hydroxyethyl methacrylate) surfaces decreased the contact angle, the dehydration rate, and the amount of nonspecific sorption of lysozyme and albumin in comparison to pristine hydrogel materials, suggesting the development of more wettable surfaces with improved water-retentive and antifouling properties, while maintaining optical transparency (>92%). In vitro testing also showed excellent viability of human corneal epithelial cells with the hyaluronic acid-grafted poly(2-hydroxyethyl methacrylate) surfaces. Hence, surface modification with hyaluronic acid via thiol-ene "click" chemistry could be useful in improving contact lens surface properties, potentially alleviating symptoms of contact lens related dryness and discomfort during wear.

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Section: Resultssupporting

confidence: 92%

Section: Ellman's Test-quantification Of Free Thiols Of Ha-shmentioning

confidence: 99%

Surface modification of model hydrogel contact lenses with hyaluronic acid via thiol-ene “click” chemistry for enhancing surface characteristics

2017

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“…These results showed the targeted ability of the HA polymer-wrapped QDs. The receptor blocked assay also confirmed the CD44 receptor imaging of HA polymer-wrapped QD-treated MCF-7 cell lines ( Wang et al, 2014 ). In another recent study, similar results were obtained for multifunctional redox-sensitive HA-modified S-S-PEGylated histamine-loaded diethylenetriamine QDs.…”

Section: Cd44 Engineered Nanocarriers For Cancer Therapysupporting

confidence: 59%

CD44-Targeted Nanocarrier for Cancer Therapy

et al. 2022

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Cluster of differentiation 44 (CD44) is a cell surface glycoprotein overexpressed in varieties of solid tumors including pancreatic, breast, ovary, brain, and lung cancers. It is a multi-structural glycoprotein of the cell surface which is majorly involved in cell proliferation, cell-to-cell interaction, cellular migration, inflammation, and generation of immune responses. Numerous studies focus on the development of nanocarriers for active targeting of the CD44 receptor to improve efficacy of targeting chemotherapy and achieve precise chemotherapy by defining the release, uptake, and accumulation of therapeutic agents. The CD44 receptor has a selective binding affinity towards hyaluronic and chondroitin sulfate (CS). Taking this into consideration, this review focused on the role of CD44 in cancer and its therapy using several nanocarriers such as polymeric/non-polymeric nanoparticles, dendrimer, micelles, carbon nanotubes, nanogels, nanoemulsions etc., for targeted delivery of several chemotherapeutic molecules and nucleic acid. This review also illuminates the role of hyaluronic acid (HA) in cancer therapy, interaction of HA with CD44, and various approaches to target CD44-overexpressed neoplastic cells.

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“…A cysteamine-modified HA polymer was employed to coat CdSe (CdZnS) QDs through a convenient one-step reverse micelle method. Prepared HA-QDs showed enhanced stability in PBS and fluorescence stability, and excellent targeting ability to CD44+ breast cancer cells without cytotoxicity, indicating that the stability and low-toxicity of QDs could be achieved through an HA coating [86]. Meanwhile, Yongbo et al prepared QDs coated by HA-magnetic Prussian Blue conjugates (HA-PB@QDs) for cancer theranosis (Figure 4).…”

Section: Ha-coated Quantum Dotmentioning

confidence: 99%

Hyaluronic Acid-Based Theranostic Nanomedicines for Targeted Cancer Therapy

Lee

Kang

Jeong

et al. 2020

Cancers

101

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Hyaluronic acid (HA) is a natural mucopolysaccharide and has many useful advantages, including biocompatibility, non-immunogenicity, chemical versatility, non-toxicity, biodegradability, and high hydrophilicity. Numerous tumor cells overexpress several receptors that have a high binding affinity for HA, while these receptors are poorly expressed in normal body cells. HA-based drug delivery carriers can offer improved solubility and stability of anticancer drugs in biological environments and allow for the targeting of cancer treatments. Based on these benefits, HA has been widely investigated as a promising material for developing the advanced clinical cancer therapies in various formulations, including nanoparticles, micelles, liposomes, and hydrogels, combined with other materials. We describe various approaches and findings showing the feasibility of improvement in theragnosis probes through the application of HA.

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Biocompatible hyaluronic acid polymer-coated quantum dots for CD44+ cancer cell-targeted imaging

Cited by 16 publications

References 56 publications

Surface modification of model hydrogel contact lenses with hyaluronic acid via thiol-ene “click” chemistry for enhancing surface characteristics

Surface modification of model hydrogel contact lenses with hyaluronic acid via thiol-ene “click” chemistry for enhancing surface characteristics

CD44-Targeted Nanocarrier for Cancer Therapy

Hyaluronic Acid-Based Theranostic Nanomedicines for Targeted Cancer Therapy

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