Biocompatible cellulose nanocrystals as supports to immobilize lipase

Kim, Hyun Jung; Park, Saerom; Kim, Sung Hee; Kim, Ji Hyun; Yu, Hyejeong; Kim, Hyung Joo; Yang, Yung‐Hun; Kan, Eunsung; Kim, Yong Hwan; Lee, Sang Hyun

doi:10.1016/j.molcatb.2015.09.007

Cited by 58 publications

(30 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This may be ascribed to the enhanced covalent bonds or hydrogen bonds formed during the immobilization process between the carrier and enzyme , leading to effective adsorption of papain and preventing its leaching, which arises usually in the case of mesoporous silica immobilization. Similarly, other immobilized enzyme also showed higher thermal stability than free enzyme (Kim et al, 2015).…”

Section: Ph and Thermal Stability Of The Free And Immobilized Papainsmentioning

confidence: 90%

Pineapple peel carboxymethyl cellulose/polyvinyl alcohol/mesoporous silica SBA-15 hydrogel composites for papain immobilization

Dai

Liu

et al. 2017

Carbohydrate Polymers

103

View full text Add to dashboard Cite

Section: Ph and Thermal Stability Of The Free And Immobilized Papainsmentioning

confidence: 90%

Pineapple peel carboxymethyl cellulose/polyvinyl alcohol/mesoporous silica SBA-15 hydrogel composites for papain immobilization

Dai

Liu

et al. 2017

Carbohydrate Polymers

103

View full text Add to dashboard Cite

“…Cellulose nanocrystals (CNCs) are rod-like nanostructures consisting of repeating glucose units, and are a subject of growing interest as key ingredients of green composites due to their mechanical and chemical properties, as well as their sustainability and biocompatibility [36][37][38][39]. CNCs are used as fillers for a variety of thermoplastics, thermosets and hydrogels, resulting in improved mechanical properties and temperature stability [40,41].…”

Section: Introductionmentioning

confidence: 99%

Shear melting and recovery of crosslinkable cellulose nanocrystal–polymer gels

et al. 2019

View full text Add to dashboard Cite

Cellulose nanocrystals (CNC) are naturally-derived nanostructures of growing importance for the production of composites having attractive mechanical properties, and offer improved sustainability over purely petroleum-based alternatives. Fabrication of CNC composites typically involves extrusion of CNC suspensions and gels in a variety of solvents, in the presence of additives such as polymers and curing agents. However, most studies so far have focused on aqueous CNC gels, yet the behavior of CNC-polymer gels in organic solvents is important to their wider processability. Here, we study the rheological behavior of composite polymer-CNC gels in dimethylformamide, which include additives for both UV and thermal crosslinking. Using rheometry coupled with in-situ infrared spectroscopy, we show that under external shear, CNC-polymer gels display progressive and irreversible failure of the hydrogen bond network that is responsible for their pronounced elastic properties. In the absence of cross-linking additives, the polymer-CNC gels show negligible recovery upon cessation of flow, while the presence of additives allows the gels to recover via van der Waals interactions. By exploring a broad range of shear history and CNC concentrations, we construct master curves for the temporal evolution of the viscoelastic properties of the polymer-CNC gels, illustrating universality of the observed dynamics with respect to gel composition and flow conditions. We therefore find that polymer-CNC composite gels display a number of the distinctive features of colloidal glasses and, strikingly, that their response to the flow conditions encountered during processing can be tuned by chemical additives. These findings have implications for processing of dense CNC-polymer composites in solvent casting, 3D printing, and other manufacturing techniques. arXiv:1901.04373v1 [cond-mat.soft]

show abstract

“…Some of the researchers used CNC for enzyme immobilization such as cyclodextrin glycosyl transferase (CGTase) and alcohol oxidase (Mahmoud et al 2009), papain (Cao et al 2014;Mahmoud et al 2013), glucose oxidase (Incani et al 2013) and Candida rugosa lipase (Kim et al 2015). Cao et al (2014) have used CNC magnetic composite for papain immobilization and they found that all the parameters studied, including pH stability, storage, enzyme activity and level of tolerance to organic solvent are higher in the immobilized form as compared to its free form.…”

Section: Introductionmentioning

confidence: 99%

Surface Modification of Cellulose Nanomaterial for Urea Biosensor Application

Khalid¹,

Lee²,

Arip³

2018

JSM

View full text Add to dashboard Cite

Cellulose nanomaterial with rod-like structure and highly crystalline order, usually formed by elimination of the amorphous region from cellulose during acid hydrolysis. Cellulose nanomaterial with the property of biocompatibility and nontoxicity can be used for enzyme immobilization. In this work, urease enzyme was used as a model enzyme to study the surface modification of cellulose nanomaterial and its potential for biosensor application. The cellulose nanocrystal (CNC) surface was modified using 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-mediated oxidation to introduce the carboxyl group at C6 primary alcohol. The success of enzyme immobilization and surface modification was confirmed using chemical tests and measured using UV-Visible spectrophotometer. The immobilization strategy was then applied for biosensor application for urea detection. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques were used for electroanalytical characterization of the urea biosensor.

show abstract

Biocompatible cellulose nanocrystals as supports to immobilize lipase

Cited by 58 publications

References 26 publications

Pineapple peel carboxymethyl cellulose/polyvinyl alcohol/mesoporous silica SBA-15 hydrogel composites for papain immobilization

Pineapple peel carboxymethyl cellulose/polyvinyl alcohol/mesoporous silica SBA-15 hydrogel composites for papain immobilization

Shear melting and recovery of crosslinkable cellulose nanocrystal–polymer gels

Surface Modification of Cellulose Nanomaterial for Urea Biosensor Application

Contact Info

Product

Resources

About