Biocompatible and Photostable Photoacoustic Contrast Agents as Nanoparticles Based on Bodipy Scaffold and Polylactide Polymers: Synthesis, Formulation, and <i>In Vivo</i> Evaluation

Bodin, Jean-Baptiste; Gâteau, Jérôme; Coïs, Justine; Lucas, Théotim; Lefèbvre, Flora; Moine, Laurence; Noiray, Magali; Cailleau, Catherine; Denis, Stéphanie; Clavier, Gilles; Tsapis, Nicolas; Méallet‐Renault, Rachel

doi:10.1021/acsami.2c04874

Cited by 6 publications

(27 citation statements)

References 58 publications

(201 reference statements)

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“…In literature, push–pull Bodipys show similar band positions. ,, Intersystem crossing ( k ISC ) could have reduced the PA signal, but according to transient absorption spectra, Bodipy does not show any triplet spectral and kinetic signatures. PAbs loss by dye degradation (bleaching) is also negligible, as demonstrated in ref , and the absorbance value was checked to remain constant between before and after the experiments. The transient absorption spectra of the NP batches NP-5% , NP-25% , and NP-50% were also recorded (Figure S21), and no new bands can be observed.…”

Section: Resultsmentioning

confidence: 98%

“…An NP hydrodynamic diameter (dynamic light scattering, DLS), zeta potential, and polydispersity index (PDI) were determined with a Zetasizer Nano ZS (Malvern, France) at 25 °C with a 632.8 nm laser (backscatter angle of 173°). The Bodipy encapsulation efficiencies were evaluated as described in ref thanks to a calibration curve (Beer–Lambert’s law) of PLA-Bodipy absorption at 757 nm in DCM as a function of the concentration (slope: ε PLA‑Bodipy = 78,000 L·mol –1 ·cm –1 , DCM, 757 nm). The mean Bodipy number per NP ( N PLA‑Bodipy ) was determined in ref by computing the ratio of the experimental Bodipy concentration C Bodipy to the nanoparticle concentration C NP (determined by nanoparticle tracking analysis).…”

Section: Methodsmentioning

confidence: 99%

“…To this attempt, we exploit a calibrated photoacoustic spectrophotometer 37 and a robust Bodipy-loaded polylactide NP recent formulation. 40 Absorption, steady state, and timeresolved emission, as well as calibrated photoacoustic spectra (at an incident fluence around 0.98 mJ•cm −2 ), were studied on PLA-Bodipy NPs with different PLA-Bodipy loadings: from 2.5 to 50% by weight. We define and introduce a new photoacoustic parameter: the global photoacoustic efficiency (GPAE), which represents the weighted average of the PGE over the whole studied band (680−870 nm).…”

Section: ■ Introductionmentioning

confidence: 99%

“…For clinical applications, it is important to limit the fluence to avoid tissue damage . Moreover, the nanoparticle concentration should also be restrained to avoid toxicity concerns . Thus, two main parameters can be tuned to enhance the photoacoustic signal: PGE and molar absorptivity.…”

Section: Introductionmentioning

confidence: 99%

“…To this attempt, we exploit a calibrated photoacoustic spectrophotometer and a robust Bodipy-loaded polylactide NP recent formulation . Absorption, steady state, and time-resolved emission, as well as calibrated photoacoustic spectra (at an incident fluence around 0.98 mJ·cm –2 ), were studied on PLA-Bodipy NPs with different PLA-Bodipy loadings: from 2.5 to 50% by weight.…”

Section: Introductionmentioning

confidence: 99%

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Fine Tuning of the Photoacoustic Generation Efficiency by Aggregation-Caused Quenching and Excitation Energy Transfer in Bodipy-Labeled Polylactide Nanoparticles

Bodin,

Linger,

Gateau

et al. 2023

J. Phys. Chem. C

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The relationship between the fluorescence decrease by aggregation-caused quenching (ACQ) and the photoacoustic generation efficiency in polylactide-Bodipy nanoparticles (PLA-Bodipy NPs) is demonstrated. PLA NPs with different PLA-Bodipy loadings (from 2.5 to 50% by weight) were studied using a calibrated photoacoustic spectrophotometer. We demonstrate the presence of two photoacoustic emission regimes thanks to the determination of the global photoacoustic efficiency (GPAE), which represents the averaged photoacoustic generation efficiency over the whole studied band (680−870 nm). In the monomer regime, below 10% by weight of PLA-Bodipy, the fluorescence emission from the Bodipy monomer limits the GPAE. Above 10% of PLA-Bodipy, in the ACQ regime, nonradiative deactivations from the aggregates are predominant and GPAE reaches a high value of 93%. We also introduce the photoacoustic brightness B PA , as the product of the GPAE by the molar extinction coefficient of NPs. When Bodipy is aggregated, high nanoparticle extinction coefficients (2.4 × 10 8 L•mol −1 •cm −1 for NP-50%) and high GPAE values are reached, leading to ultrabright NPs (22 × 10 7 L•mol −1 •cm −1 ). Finally, we show that high laser fluences (1−3.5 mJ•cm −2 ) can significantly reduce the photoacoustic signal by ground state depopulation at the band maximum excitation (−20% for NP-2.5%). This nonlinear effect can be highly reduced with NPs in the ACQ regime. Above 10% PLA-Bodipy, NPs exhibit intense photoacoustic brightness and low signal loss by nonlinear effects. A mathematical fit of the absorption and photoacoustic excitation spectra allowed us to introduce the band photoacoustic efficiency (BPAE) to spectrally differentiate the averaged photoacoustic efficiency at the band maximum BPAE red and in the vibrational shoulder BPAE blue . The BPAE red highly depends on the laser fluence due to high-ground state depopulation, whereas the BPAE blue does not change with the fluence because of a lower laser fluence saturation.

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Section: Resultsmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fine Tuning of the Photoacoustic Generation Efficiency by Aggregation-Caused Quenching and Excitation Energy Transfer in Bodipy-Labeled Polylactide Nanoparticles

Bodin,

Linger,

Gateau

et al. 2023

J. Phys. Chem. C

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Supramolecular dye nanoassemblies for advanced diagnostics and therapies

Ramezani,

De Smedt,

Sauvage

2024

Bioengineering & Transla Med

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Dyes have conventionally been used in medicine for staining cells, tissues, and organelles. Since these compounds are also known as photosensitizers (PSs) which exhibit photoresponsivity upon photon illumination, there is a high desire towards formulating these molecules into nanoparticles (NPs) to achieve improved delivery efficiency and enhanced stability for novel imaging and therapeutic applications. Furthermore, it has been shown that some of the photophysical properties of these molecules can be altered upon NP formation thereby playing a major role in the outcome of their application. In this review, we primarily focus on introducing dye categories, their formulation strategies and how these strategies affect their photophysical properties in the context of photothermal and non‐photothermal applications. More specifically, the most recent progress showing the potential of dye supramolecular assemblies in modalities such as photoacoustic and fluorescence imaging, photothermal and photodynamic therapies as well as their employment in photoablation as a novel modality will be outlined. Aside from their photophysical activity, we delve shortly into the emerging application of dyes as drug stabilizing agents where these molecules are used together with aggregator molecules to form stable nanoparticles.

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Drug delivery of Mechlorethamine as anticancer drug by Silicon, Carbon and Aluminum Nitride nanocages

Mekkey,

Al-dolaimy,

Hussein

et al. 2023

Preprint

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The adsorption of Mechlorethamine on silicon, carbon and aluminum nitride (Si76, C76 and Al38N38) nanocages are investigated. The effects of V adoption on potential of Si76, C76 and Al38N38 nanocages to delivery the Mechlorethamine are investigated. The adsorption energy, Gibbs free energy, recovery time, orbital gap energy, charge transfer of interactions of Si76, C76, Al38N38, V-Si76, V-C76 and V-Al38N38 nanocages with Mechlorethamine are calculated. Results shown that the Si76 and Al38N38 nanocage has higher potential to Mechlorethamine delivery than C76 nanocage. The V adoption of nanocages can increase the interactions of Si76, C76 and Al38N38 nanocages with Mechlorethamine and their abilities to drug delivery. Finally, results are demonstrated that the V-Si76 and V-Al38N38 are acceptable nanocages to delivery of Mechlorethamine with high performance.

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Biocompatible and Photostable Photoacoustic Contrast Agents as Nanoparticles Based on Bodipy Scaffold and Polylactide Polymers: Synthesis, Formulation, and In Vivo Evaluation

Cited by 6 publications

References 58 publications

Fine Tuning of the Photoacoustic Generation Efficiency by Aggregation-Caused Quenching and Excitation Energy Transfer in Bodipy-Labeled Polylactide Nanoparticles

Fine Tuning of the Photoacoustic Generation Efficiency by Aggregation-Caused Quenching and Excitation Energy Transfer in Bodipy-Labeled Polylactide Nanoparticles

Supramolecular dye nanoassemblies for advanced diagnostics and therapies

Drug delivery of Mechlorethamine as anticancer drug by Silicon, Carbon and Aluminum Nitride nanocages

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